2IJI: F14H mutant of ColE1 Rom protein

Structure of F14H mutant of ColE1 Rom protein. Determined by X-ray diffraction at 2.3 Å resolution. Released 16 Oct 2007.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: Escherichia coli
Chains: 1
Atoms: 457
Mol. weight: 7.15 kDa
Released: 16 Oct 2007

Explore 2IJI in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2IJI contains 2 α-helices and 0 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	3-28	26
α-helix	33-55	23

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Regulatory protein rop	A	protein	63	Escherichia coli	P03051 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2IJI_1 Regulatory protein rop (chains A)
GTKQEKTALNMARHIRSQTLTLLEKLNELDADEQADICESLHDHADELYRSCLARFGDDG
ENL

Primary citation

New crystal structures of ColE1 Rom and variants resulting from mutation of a surface exposed residue: Implications for RNA-recognition. Struble, E.B., Ladner, J.E., Brabazon, D.M. et al. Proteins (2008) 72:761-768. DOI 10.1002/prot.21965 · PubMed

Other PDB entries of the same protein (UniProt P03051 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1NKD 1.09 Å, Atomic resolution (1.07 Å) structure of the rop mutant <2AA>
1RPO 1.4 Å, Restored heptad pattern continuity does not alter the folding of a 4-alpha-helical bundle
4DO2 1.4 Å, Crystal Structure of the Rop protein mutant D30P/A31G at resolution 1.4 resolution.
2IJK 1.55 Å, Structure of a Rom protein dimer at 1.55 angstrom resolution
7KAE 1.6 Å, Rop protein variant with a buried tryptophan
1ROP 1.7 Å, Structure of the COL*E1 rop protein at 1.7 Å resolution
1B6Q 1.8 Å, Alanine 31 proline mutant of rop protein
2IJH 1.8 Å, Crystal structure analysis of ColE1 ROM mutant F14W
1GTO 1.82 Å, High resolution structure of a hyperstable helical bundle protein mutant
1F4N 1.9 Å, C2 crystal structure of ALA2ILE2-6, a version of rop with a repacked hydrophobic core…
1GMG 1.9 Å, Alanine 31 proline mutant of rop protein, monoclinic form
2IJJ 1.9 Å, Crystal structure analysis of ColE1 ROM mutant F14Y

Browse structure collections

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