2PBD: Actin, alpha skeletal muscle

Ternary complex of profilin-actin with the poly-PRO-GAB domain of VASP*. Determined by X-ray diffraction at 1.5 Å resolution. Released 13 Nov 2007.

Method: X-ray diffraction
Resolution: 1.5 Å
Organisms: Oryctolagus cuniculus, Homo sapiens
Chains: 3
Atoms: 5,029
Mol. weight: 61.47 kDa
Ligands: ATP, CA
Released: 13 Nov 2007

Explore 2PBD in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

2PBD contains 35 α-helices and 28 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 27 helices, 20 β-strands

Element	Residues	Length	Sheet
α-helix	6-7	2
β-strand	8-12	5	1
β-strand	16-21	6	1
β-strand	24	1	2
β-strand	29-32	4	1
β-strand	35-37	3	3
β-strand	53-54	2	3
α-helix	56-60	5
α-helix	62-64	3
β-strand	66-68	3	3
β-strand	71-72	2	4
β-strand	75-76	2	4
α-helix	79-87	9
α-helix	88-94	7
α-helix	98-100	3
β-strand	103-107	5	1
α-helix	113-121	9
α-helix	122-126	5
β-strand	131-136	6	1
α-helix	137-144	8
β-strand	150-155	6	5
β-strand	160-166	7	5
β-strand	169-170	2	5
α-helix	172-174	3
β-strand	176-178	3	5
α-helix	182-196	15
α-helix	203-216	14
α-helix	223-229	7
α-helix	230-232	3
β-strand	238-241	4	6
β-strand	247-250	4	6
α-helix	253-259	7
α-helix	264-267	4
α-helix	274-283	10
α-helix	287-289	3
α-helix	290-294	5
β-strand	297-300	4	5
α-helix	302-304	3
α-helix	309-320	12
α-helix	322-324	3
α-helix	327-329	3
β-strand	330	1	5
α-helix	338-348	11
α-helix	350-352	3
β-strand	357-358	2	1
α-helix	359-365	7
α-helix	369-373	5

Chain P: 6 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	4-11	8
β-strand	16-23	8	7
β-strand	29-33	5	7
α-helix	39-41	3
α-helix	44-51	8
α-helix	57-59	3
β-strand	63-65	3	7
β-strand	68-76	9	7
β-strand	84-89	6	7
β-strand	99-104	6	7
β-strand	108-114	7	7
α-helix	115	1
α-helix	120-136	17

Chain V: 2 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	203-210	8
α-helix	225-230	6
β-strand	235	1	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin, alpha skeletal muscle	A	protein	377	Oryctolagus cuniculus	P68135 (AlphaFold model)
Profilin-1	P	protein	139	Homo sapiens	P07737 (AlphaFold model)
Vasodilator-stimulated phosphoprotein	V	protein	43		P50552 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>2PBD_1 Actin, alpha skeletal muscle (chains A)
MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
MSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIT
KQEYDEAGPSIVHRKCF

Sequence of entity 2 (P), FASTA

>2PBD_2 Profilin-1 (chains P)
AGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYV
NGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHG
GLINKKCYEMASHLRRSQY

Sequence of entity 3 (V), FASTA

>2PBD_3 Vasodilator-stimulated phosphoprotein (chains V)
GPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEAS

Ligands and cofactors

ID	Name	Formula	Copies
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1
CA	Calcium ion	Ca	1

Primary citation

Structural basis for the recruitment of profilin-actin complexes during filament elongation by Ena/VASP. Ferron, F., Rebowski, G., Lee, S.H. et al. EMBO J (2007) 26:4597-4606. DOI 10.1038/sj.emboj.7601874 · PubMed

Other PDB entries of the same protein (UniProt P68135 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4B1Y 1.29 Å, Structure of the Phactr1 RPEL-3 bound to G-actin
2FXU 1.35 Å, X-ray Structure of Bistramide A- Actin Complex at 1.35 A resolution.
4K41 1.4 Å, Crystal structure of actin in complex with marine macrolide kabiramide C
1QZ5 1.45 Å, Structure of rabbit actin in complex with kabiramide C
1WUA 1.45 Å, The structure of Aplyronine A-actin complex
2Q0U 1.45 Å, Structure of Pectenotoxin-2 and Latrunculin B Bound to Actin
2V52 1.45 Å, Structure of MAL-RPEL2 complexed to G-actin
3MN5 1.5 Å, Structures of actin-bound WH2 domains of Spire and the implication for filament nucleation
5ZZA 1.53 Å, OdinProfilin/Rabbit Actin Complex
1J6Z 1.54 Å, Uncomplexed actin
1QZ6 1.6 Å, Structure of rabbit actin in complex with jaspisamide A
1S22 1.6 Å, Absolute Stereochemistry of Ulapualide A

Browse structure collections

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