31FX: Mouse 80S ribosome, sgRNA control

Name: PDB 31FX: Mouse 80S ribosome, sgRNA control
Published: 2026-09-23
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: ribosome

Mouse 80S ribosome, sgRNA control. Determined by electron microscopy at 2.54 Å resolution. Released 23 Sept 2026.

Method: Electron microscopy
Resolution: 2.54 Å
Organism: Mus musculus
Chains: 79
Atoms: 208,385
Mol. weight: 3683.27 kDa
Ligands: ZN
Released: 23 Sept 2026

Explore 31FX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

31FX contains 570 α-helices and 517 β-strands across 75 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain Aa: 4 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	6-20	15
β-strand	24-27	4	7
α-helix	32-43	12
β-strand	47-53	7	7
β-strand	60-65	6	7
β-strand	72-74	3	8
β-strand	80-81	2	8
α-helix	83-93	11
β-strand	101-106	6	8
β-strand	109-112	4	8
α-helix	113-118	6
β-strand	123-129	7	8

Chain AA: 0 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	10-21	12	1
β-strand	27-34	8	1
β-strand	41-47	7	1
β-strand	55-58	4	1

Chain Ab: 8 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	6-15	10	14
β-strand	20-28	9	14
α-helix	33-36	4
α-helix	37-48	12
α-helix	52-54	3
β-strand	55-62	8	14
α-helix	63	1
β-strand	68-76	9	14
α-helix	79-84	6
α-helix	88-94	7
α-helix	104-115	12
α-helix	119-121	3

Chain AB: 3 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	16-18	3
β-strand	30-31	2	3
α-helix	33-35	3
β-strand	38-39	2	3
α-helix	40-50	11
β-strand	53	1	4

Chain Ac: 4 helices, 5 β-strands

Element	Residues	Length	Sheet
β-strand	47	1	76
α-helix	52-60	9
α-helix	62-64	3
β-strand	67-68	2	77
α-helix	70-77	8
β-strand	79	1	76
α-helix	81-94	14
β-strand	97-103	7	77
β-strand	106-111	6	77

Chain Ad: 2 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	12-17	6
β-strand	32-36	5	2
β-strand	44-47	4	2
β-strand	54-55	2	17
α-helix	56	1
β-strand	62-66	5	17
β-strand	71-73	3	17
β-strand	78-81	4	2

Chain AD: 10 helices, 10 β-strands

Element	Residues	Length	Sheet
α-helix	24-27	4
β-strand	28-34	7	87
β-strand	43-50	8	87
α-helix	58-62	5
β-strand	66-70	5	87
α-helix	71-73	3
β-strand	82-92	11	87
β-strand	95-105	11	87
α-helix	107-113	7
β-strand	120-128	9	87
β-strand	134-142	9	87
β-strand	143	1	88
α-helix	155-157	3
α-helix	158-177	20
α-helix	181-188	8
α-helix	192-201	10
β-strand	207	1	88
β-strand	211-217	7	87
α-helix	221-223	3
α-helix	225-233	9

Chain Ae: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	85-89	5

67 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Small ribosomal subunit protein eS28	AA	protein	69	Mus musculus	P62858 (AlphaFold model)
Small ribosomal subunit protein uS14	AB	protein	56	Mus musculus	P62274 (AlphaFold model)
18S rRNA	AC	RNA	1871	Mus musculus
Small ribosomal subunit protein uS3	AE	protein	243	Mus musculus	P62908 (AlphaFold model)
Small ribosomal subunit protein eS17	AH	protein	135	Mus musculus	P63276 (AlphaFold model)
Small ribosomal subunit protein uS7	AJ	protein	204	Mus musculus	P97461
Small ribosomal subunit protein eS10	AK	protein	165	Mus musculus	P63325
Small ribosomal subunit protein uS19	AL	protein	145	Mus musculus	P62843
Small ribosomal subunit protein uS9	AM	protein	146	Mus musculus	P14131
Small ribosomal subunit protein uS13	AN	protein	152	Mus musculus	P62270
Small ribosomal subunit protein eS19	AO	protein	145	Mus musculus	Q9CZX8
Small ribosomal subunit protein uS10	AP	protein	119	Mus musculus	P60867

67 more molecules are not listed.

Sequence of entity 1 (AA), FASTA

>31FX_1 Small ribosomal subunit protein eS28 (chains AA)
MDTSRVQPIKLARVTKVLGRTGSQGQCTQVRVEFMDDTSRSIIRNVKGPVREGDVLTLLE
SEREARRLR

Sequence of entity 2 (AB), FASTA

>31FX_2 Small ribosomal subunit protein uS14 (chains AB)
MGHQQLYWSHPRKFGQGSRSCRVCSNRHGLIRKYGLNMCRQCFRQYAKDIGFIKLD

Sequence of entity 3 (AC), FASTA

>31FX_3 18S rRNA (chains AC)
UACCUGGUUGAUCCUGCCAGUAGCAUAUGCUUGUCUCAAAGAUUAAGCCAUGCAUGUCUA
AGUACGCACGGCCGGUACAGUGAAACUGCGAAUGGCUCAUUAAAUCAGUUAUGGUUCCUU
UGGUCGCUCGCUCCUCUCCUACUUGGAUAACUGUGGUAAUUCUAGAGCUAAUACAUGCCG
ACGGGCGCUGACCCCCCUUCCCGGGGGGGGAUGCGUGCAUUUAUCAGAUCAAAACCAACC
CGGUGAGCUCCCUCCCGGCUCCGGCCGGGGGUCGGGCGCCGGCGGCUUGGUGACUCUAGA
UAACCUCGGGCCGAUCGCACGCCCCCCGUGGCGGCGACGACCCAUUCGAACGUCUGCCCU
AUCAACUUUCGAUGGUAGUCGCCGUGCCUACCAUGGUGACCACGGGUGACGGGGAAUCAG
GGUUCGAUUCCGGAGAGGGAGCCUGAGAAACGGCUACCACAUCCAAGGAAGGCAGCAGGC
GCGCAAAUUACCCACUCCCGACCCGGGGAGGUAGUGACGAAAAAUAACAAUACAGGACUC
UUUCGAGGCCCUGUAAUUGGAAUGAGUCCACUUUAAAUCCUUUAACGAGGAUCCAUUGGA
GGGCAAGUCUGGUGCCAGCAGCCGCGGUAAUUCCAGCUCCAAUAGCGUAUAUUAAAGUUG
CUGCAGUUAAAAAGCUCGUAGUUGGAUCUUGGGAGCGGGCGGGCGGUCCGCCGCGAGGCG
AGUCACCGCCCGUCCCCGCCCCUUGCCUCUCGGCGCCCCCUCGAUGCUCUUAGCUGAGUG
UCCCGCGGGGCCCGAAGCGUUUACUUUGAAAAAAUUAGAGUGUUCAAAGCAGGCCCGAGC
CGCCUGGAUACCGCAGCUAGGAAUAAUGGAAUAGGACCGCGGUUCUAUUUUGUUGGUUUU
CGGAACUGAGGCCAUGAUUAAGAGGGACGGCCGGGGGCAUUCGUAUUGCGCCGCUAGAGG
UGAAAUUCUUGGACCGGCGCAAGACGGACCAGAGCGAAAGCAUUUGCCAAGAAUGUUUUC
AUUAAUCAAGAACGAAAGUCGGAGGUUCGAAGACGAUCAGAUACCGUCGUAGUUCCGACC
AUAAACGAUGCCGACUGGCGAUGCGGCGGCGUUAUUCCCAUGACCCGCCGGGCAGCUUCC
GGGAAACCAAAGUCUUUGGGUUCCGGGGGGAGUAUGGUUGCAAAGCUGAAACUUAAAGGA
AUUGACGGAAGGGCACCACCAGGAGUGGAGCCUGCGGCUUAAUUUGACXCAACACGGGAA
ACCUCACCCGGCCCGGACACGGACAGGAUUGACAGAUUGAUAGCUCUUUCUCGAUUCCGU
GGGUGGUGGUGCAUGGCCGUUCUUAGUUGGUGGAGCGAUUUGUCUGGUUAAUUCCGAUAA
CGAACGAGACUCUGGCAUGCUAACUAGUUACGCGACCCCCGAGCGGUCGGCGUCCCCCAA
CUUCUUAGAGGGACAAGUGGCGUUCAGCCACCCGAGAUUGAGCAAUAACAGGUCUGUGAU
GCCCUUAGAUGUCCGGGGCUGCACGCGCGCUACACUGACUGGCUCAGCGUGUGCCUACCC
UGCGCCGGCAGGCGCGGGUAACCCGUUGAACCCCAUUCGUGAUGGGGAUCGGGGAUUGCA
AUUAUUCCCCAUGAACGAGGAAUUCCCAGUAAGUGCGGGUCAUAAGCUUGCGUUGAUUAA
GUCCCUGCCCUUUGUACACACCGCCCGUCGCUACUACCGAUUGGAUGGUUUAGUGAGGCC
CUCGGAUCGGCCCCGCCGGGGUCGGCCCACGGCCCUGGCGGAGCGCUGAGAAGACGGUCG
AACUUGACUAUCUAGAGGAAGUAAAAGUCGUAACAAGGUUUCNGUAGGUGAACCUGCGGA
AGGAUCAUUAA

Sequence of entity 4 (AE), FASTA

>31FX_4 Small ribosomal subunit protein uS3 (chains AE)
MAVQISKKRKFVADGIFKAELNEFLTRELAEDGYSGVEVRVTPTRTEIIILATRTQNVLG
EKGRRIRELTAVVQKRFGFPEGSVELYAEKVATRGLCAIAQAESLRYKLLGGLAVRRACY
GVLRFIMESGAKGCEVVVSGKLRGQRAKSMKFVDGLMIHSGDPVNYYVDTAVRHVLLRQG
VLGIKVKIMLPWDPSGKIGPKKPLPDHVSIVEPKDEILPTTPISEQKGGKPEPPAMPQPV
PTA

Sequence of entity 5 (AH), FASTA

>31FX_5 Small ribosomal subunit protein eS17 (chains AH)
MGRVRTKTVKKAARVIIEKYYTRLGNDFHTNKRVCEEIAIIPSKKLRNKIAGYVTHLMKR
IQRGPVRGISIKLQEEERERRDNYVPEVSALDQEIIEVDPDTKEMLKLLDFGSLSNLQVT
QPTVGMNFKTPRGAV

Sequence of entity 6 (AJ), FASTA

>31FX_6 Small ribosomal subunit protein uS7 (chains AJ)
MTEWEAATPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKR
FRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSG
PREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINA
AKGSSNSYAIKKKDELERVAKSNR

Sequence of entity 7 (AK), FASTA

>31FX_7 Small ribosomal subunit protein eS10 (chains AK)
MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKE
QFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGPEGERPARFTRG
EADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Sequence of entity 8 (AL), FASTA

>31FX_8 Small ribosomal subunit protein uS19 (chains AL)
MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRL
RKAKKEAPPMEKPEVVKTHLRDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFS
ITYKPVKHGRPGIGATHSSRFIPLK

Sequence of entity 9 (AM), FASTA

>31FX_9 Small ribosomal subunit protein uS9 (chains AM)
MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGK
ERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLL
VADPRRCESKKFGGPGARARYQKSYR

Sequence of entity 10 (AN), FASTA

>31FX_10 Small ribosomal subunit protein uS13 (chains AN)
MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELT
EDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAH
RGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

Sequence of entity 11 (AO), FASTA

>31FX_11 Small ribosomal subunit protein eS19 (chains AO)
MPGVTVKDVNQQEFVRALAAFLKKSGKLKVPEWVDTVKLAKHKELAPYDENWFYTRAAST
ARHLYLRGGAGVGSMTKIYGGRQRNGVRPSHFSRGSKSVARRVLQALEGLKMVEKDQDGG
RKLTPQGQRDLDRIAGQVAAANKKH

Sequence of entity 12 (AP), FASTA

>31FX_12 Small ribosomal subunit protein uS10 (chains AP)
MAFKDTGKTPVEPEVAIHRIRITLTSRNVKSLEKVCADLIRGAKEKNLKVKGPVRMPTKT
LRITTRKTPCGEGSKTWDRFQMRIHKRLIDLHSPSEIVKQITSISIEPGVEVEVTIADA

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	7

Primary citation

Gm10076 and eL41. Rabl, J., Valdivia Francia, F., Sendoel, A. To be published.

Other PDB entries of the same protein (UniProt P62858 (AlphaFold model), which also has an AlphaFold model), best resolution first:

31FY 2.57 Å, Mouse 80S ribosome, Gm10076 KO
9KDT 2.63 Å, Cryo-EM structure of 80S ribosome
9KDW 2.67 Å, Cryo-EM structure of 80S ribosome
9QOH 2.78 Å, Mouse Ribosome POST translocation state
9QQP 2.8 Å, Mouse Ribosome rotated-2 PRE state
7CPU 2.82 Å, Cryo-EM structure of 80S ribosome from mouse kidney
31FZ 2.89 Å, Mouse 80S ribosome, eL41 KO
9KDU 2.96 Å, Cryo-EM structure of 80S ribosome
7CPV 3.03 Å, Cryo-EM structure of 80S ribosome from mouse testis
9QQL 3.09 Å, Mouse RPS15 P131 Mutant Ribosome POST translocation state
7LS2 3.1 Å, 80S ribosome from mouse bound to eEF2 (Class I)
9QWT 3.1 Å, Mouse Ribosome RPS15 (uS19) P131S rotated-2 PRE state

Browse structure collections

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