3AH8: Heterotrimeric G protein Galpha-q beta gamma

Structure of heterotrimeric G protein Galpha-q beta gamma in complex with an inhibitor YM-254890. Determined by X-ray diffraction at 2.9 Å resolution. Released 21 Jul 2010.

Method: X-ray diffraction
Resolution: 2.9 Å
Organisms: Rattus norvegicus, Mus musculus, Bos taurus
Chains: 4
Atoms: 5,827
Mol. weight: 88.84 kDa
Ligands: GDP
Released: 21 Jul 2010

Explore 3AH8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3AH8 contains 24 α-helices and 40 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	19-37	19
β-strand	40-46	7	1
α-helix	52-62	11
α-helix	69-96	28
α-helix	106-114	9
α-helix	126-137	12
α-helix	139-146	8
α-helix	157-161	5
α-helix	164-167	4
α-helix	176-179	4
β-strand	190-196	7	1
β-strand	199-205	7	1
α-helix	213-215	3
α-helix	217-220	4
β-strand	225-231	7	1
α-helix	233-236	4
α-helix	247-260	14
α-helix	262-264	3
β-strand	268-274	7	1
α-helix	276-282	7
α-helix	289-291	3
α-helix	302-315	14
β-strand	324-328	5	1
α-helix	334-352	19

Chain B: 3 helices, 34 β-strands

Element	Residues	Length	Sheet
α-helix	15-25	11
α-helix	30-33	4
β-strand	47-52	6	2
β-strand	58	1	3
β-strand	62-63	2	3
β-strand	69-71	3	3
β-strand	74	1	3
β-strand	78-83	6	3
β-strand	89-94	6	3
β-strand	100-103	4	4
β-strand	111-116	6	4
β-strand	120	1	5
β-strand	122-125	4	4
β-strand	134-137	4	4
β-strand	140	1	5
β-strand	146-153	8	6
β-strand	156-161	6	6
β-strand	165-170	6	6
β-strand	175-181	7	6
α-helix	186	1
β-strand	187	1	7
β-strand	191-192	2	8
β-strand	199-201	3	8
β-strand	203	1	7
β-strand	207-211	5	8
β-strand	218-223	6	8
β-strand	229-234	6	9
β-strand	240-245	6	9
β-strand	250-254	5	9
β-strand	259-264	6	9
β-strand	273-278	6	10
β-strand	284-289	6	10
β-strand	293-298	6	10
β-strand	304-309	6	10
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain G: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	22-30	9
α-helix	37-49	13
α-helix	52-54	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-1/Guanine nucleotide-binding protein G(q)…	A	protein	355	Rattus norvegicus, Mus musculus	P10824 (AlphaFold model), P21279 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	340	Bos taurus	P62871 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	G	protein	78	Bos taurus	P63212 (AlphaFold model)
YM-254890	Y	protein	9	Chromobacterium sp.

Sequence of entity 1 (A), FASTA

>3AH8_1 Guanine nucleotide-binding protein G(i) subunit alpha-1/Guanine nucleotide-binding protein G(q) subunit alpha chimeric protein (chains A)
GAMGCTLSAEDKAAVERSKMIDRNLREDGERSRRELKLLLLGTGESGKSTFIKQMRIIHG
SGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAF
ENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPSYLPTQQDVLRVRV
PTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDN
ENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQA
AREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 2 (B), FASTA

>3AH8_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (G), FASTA

>3AH8_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains G)
GAMDPEFMASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTP
VPASENPFREKKFFSAIL

Sequence of entity 4 (Y), FASTA

>3AH8_4 YM-254890 (chains Y)
XLLTTXXAA

Ligands and cofactors

ID	Name	Formula	Copies
GDP	Guanosine-5'-diphosphate	C10 H15 N5 O11 P2	1

Primary citation

Structural basis for the specific inhibition of heterotrimeric Gq protein by a small molecule. Nishimura, A., Kitano, K., Takasaki, J. et al. Proc Natl Acad Sci U S A (2010) 107:13666-13671. DOI 10.1073/pnas.1003553107 · PubMed

Other PDB entries of the same protein (UniProt P10824 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1CIP 1.5 Å, Gi-alpha-1 subunit of guanine nucleotide-binding protein complexed with a GTP analogue
1SVS 1.5 Å, Structure of the K180P mutant of Gi alpha subunit bound to GppNHp.
4N0D 1.55 Å, Crystal structure of the K345L variant of the Gi alpha1 subunit bound to GTPgammaS
5KDO 1.9 Å, Heterotrimeric complex of the 4 alanine insertion variant of the Gi alpha1 subunit and…
1AS0 2.0 Å, GTP-gamma-S bound G42V GIA1
1GIA 2.0 Å, Structure of active conformations of GIA1 and the mechanism of GTP hydrolysis
1SVK 2.0 Å, Structure of the K180P mutant of Gi alpha subunit bound to AlF4 and GDP
4PAQ 2.0 Å, A conserved phenylalanine as relay between the 5 helix and the GDP binding region of…
4PAO 2.0 Å, A conserved phenylalanine as relay between the 5 helix and the GDP binding region of…
1FQJ 2.02 Å, Crystal structure of the heterotrimeric complex of the rgs domain of RGS9, the gamma…
6M8H 2.07 Å, Crystal Structure of the R208Q mutant of G(i) subunit alpha-1
1BH2 2.1 Å, A326S mutant of an inhibitory alpha subunit

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