3OJ3: A20 ZnF4 and ubiquitin complex

Crystal structure of the A20 ZnF4 and ubiquitin complex. Determined by X-ray diffraction at 2.5 Å resolution. Released 8 Dec 2010.

Method: X-ray diffraction
Resolution: 2.5 Å
Organism: Homo sapiens
Chains: 16
Atoms: 6,729
Mol. weight: 114.74 kDa
Ligands: ZN
Released: 8 Dec 2010

Explore 3OJ3 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3OJ3 contains 40 α-helices and 86 β-strands across 16 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A, B, C, D, E and F: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	1
β-strand	12-16	5	1
β-strand	22	1	2
α-helix	23-34	12
β-strand	41-45	5	1
β-strand	48-49	2	1
α-helix	50-51	2
β-strand	55	1	2
α-helix	57-59	3
β-strand	66-71	6	1

Chains G and H: 3 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	13
β-strand	12-16	5	13
β-strand	22	1	14
α-helix	23-34	12
β-strand	42-45	4	13
β-strand	48-49	2	13
α-helix	50-51	2
β-strand	55	1	14
α-helix	57-59	3
β-strand	66-70	5	13

Chains I, L, M and P: 2 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	606	1	17
β-strand	615	1	17
β-strand	617	1	18
α-helix	618-620	3
β-strand	623	1	18
α-helix	625-634	10

Chains J, K and N: 2 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	606	1	19
β-strand	615	1	19
β-strand	617	1	20
α-helix	618-620	3
β-strand	623	1	20
α-helix	625-633	9

Chain O: 2 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	606	1	29
β-strand	615	1	29
α-helix	618-620	3
α-helix	625-634	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ubiquitin	A, B, C, D, E, F, G, H	protein	79	Homo sapiens	P0CG47 (AlphaFold model)
Tumor necrosis factor alpha-induced protein 3	I, J, K, L, M, N, O, P	protein	49	Homo sapiens	P21580 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F, G, H), FASTA

>3OJ3_1 Ubiquitin (chains A, B, C, D, E, F, G, H)
GSHMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLS
DYNIQKESTLHLVLRLRGG

Sequence of entity 2 (I, J, K, L, M, N, O, P), FASTA

>3OJ3_2 Tumor necrosis factor alpha-induced protein 3 (chains I, J, K, L, M, N, O, P)
GSPEFSQAARTPGDRTGTSKCRKAGCVYFGTPENKGFCTLCFIEYRENK

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	8

Primary citation

Ubiquitin Binding to A20 ZnF4 Is Required for Modulation of NF-KB Signaling. Bosanac, I., Wertz, I.E., Pan, B. et al. Mol Cell (2010) 40:548-557. DOI 10.1016/j.molcel.2010.10.009 · PubMed

Other PDB entries of the same protein (UniProt P0CG47 (AlphaFold model), which also has an AlphaFold model), best resolution first:

5NVG 1.07 Å, Thr12 Phosphorylated Ubiquitin
5TOG 1.08 Å, Room temperature structure of ubiquitin variant u7ub25.2540
5TOF 1.12 Å, Room temperature structure of ubiquitin variant u7ub25
4XOF 1.15 Å, Observing the overall rocking motion of a protein in a crystal - Orthorhombic Ubiquitin…
5GOD 1.15 Å, Lys27-linked di-ubiquitin
5GOB 1.15 Å, Lys6-linked di-ubiquitin
5W46 1.18 Å, Structure of S65D Phosphomimetic Ubiquitin Refined at 1.2 Angstroms Resolution
7S6O 1.25 Å, The crystal structure of Lys48-linked di-ubiquitin
8IC9 1.25 Å, Lys48-linked K48C-diubiquitin
5DK8 1.32 Å, Human ubiquitin in the P1 space group
7CAP 1.33 Å, Cyclic Lys48-linked triubiquitin
5V1Y 1.42 Å, Crystal structure of the ternary RPN13 PRU-RPN2 (940-953)-ubiquitin complex

Browse structure collections

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