3P36: Polo-like kinase I Polo-box domain

Polo-like kinase I Polo-box domain in complex with DPPLHSpTA phosphopeptide from PBIP1. Determined by X-ray diffraction at 1.59 Å resolution. Released 27 Apr 2011.

Method: X-ray diffraction
Resolution: 1.59 Å
Organism: Homo sapiens
Chains: 2
Atoms: 2,067
Mol. weight: 27.88 kDa
Ligands: PO4
Released: 27 Apr 2011

Explore 3P36 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3P36 contains 7 α-helices and 14 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 13 β-strands

Element	Residues	Length	Sheet
α-helix	373-386	14
α-helix	397-400	4
β-strand	401	1	1
α-helix	403-405	3
β-strand	411-417	7	2
β-strand	422-427	6	2
β-strand	432-436	5	2
β-strand	441-444	4	2
β-strand	450-454	5	2
β-strand	460-464	5	2
α-helix	470-472	3
α-helix	473-489	17
β-strand	511-516	6	1
β-strand	520-525	6	1
β-strand	530-534	5	1
β-strand	540-544	5	1
β-strand	549-553	5	1
β-strand	559-563	5	1
α-helix	564-570	7
α-helix	574-591	18

Chain B: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	76	1	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Serine/threonine-protein kinase PLK1	A	protein	232	Homo sapiens	P53350 (AlphaFold model)
phosphopeptide	B	protein	10

Sequence of entity 1 (A), FASTA

>3P36_1 Serine/threonine-protein kinase PLK1 (chains A)
GPLGSPEFDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYG
LGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYF
RNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLI
LCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSR

Sequence of entity 2 (B), FASTA

>3P36_2 phosphopeptide (chains B)
XDPPLHSTAX

Ligands and cofactors

ID	Name	Formula	Copies
PO4	Phosphate ion	O4 P	1

Water and common crystallization additives (GOL) are not listed.

Primary citation

From crystal packing to molecular recognition: prediction and discovery of a binding site on the surface of polo-like kinase 1. Sledz, P., Stubbs, C.J., Lang, S. et al. Angew Chem Int Ed Engl (2011) 50:4003-4006. DOI 10.1002/anie.201008019 · PubMed

Other PDB entries of the same protein (UniProt P53350 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4X9R 1.4 Å, PLK-1 polo-box domain in complex with Bioactive Imidazolium-containing phosphopeptide…
3P34 1.4 Å, Polo-like kinase I Polo-box domain in complex with MQSpTPL phosphopeptide
3Q1I 1.4 Å, Polo-like kinase I Polo-box domain in complex with FMPPPMSpSM phosphopeptide from TCERG1
4X9V 1.43 Å, PLK-1 polo-box domain in complex with Bioactive Imidazolium-containing phosphopeptide…
4O6W 1.45 Å, Peptide-Based Inhibitors of Plk1 Polo-box Domain
6AX4 1.45 Å, Plk-1 polo-box domain in complex with histidine N(tau)-cyclized Macrocycle 5b.
3RQ7 1.55 Å, Polo-like kinase 1 Polo box domain in complex with a C6H5(CH2)8-derivatized peptide…
4DFW 1.55 Å, Oxime-based Post Solid-phase Peptide Diversification: Identification of High Affinity…
3FVH 1.58 Å, Polo-like kinase 1 Polo box domain in complex with Ac-LHSpTA-NH2 peptide
4LKL 1.58 Å, Crystal structure of Plk1 Polo-box domain in complex with PL-55
4RCP 1.6 Å, Crystal structure of Plk1 Polo-box domain in complex with PL-2
9QMO 1.6 Å, PLK1 PBD apo protein at 1.6A

Browse structure collections

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