3SSX: E. coli trp aporeporessor L75F mutant

E. coli trp aporeporessor L75F mutant. Determined by X-ray diffraction at 1.58 Å resolution. Released 20 Jul 2011.

Method: X-ray diffraction
Resolution: 1.58 Å
Organism: Escherichia coli
Chains: 2
Atoms: 1,933
Mol. weight: 24.71 kDa
Ligands: TAM
Released: 20 Jul 2011

Explore 3SSX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3SSX contains 13 α-helices and 0 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain N: 6 helices, 0 β-strands

Element	Residues	Length
α-helix	9-31	23
α-helix	35-42	8
α-helix	45-63	19
α-helix	68-75	8
α-helix	79-91	13
α-helix	94-105	12

Chain R: 7 helices, 0 β-strands

Element	Residues	Length
α-helix	9-31	23
α-helix	35-42	8
α-helix	45-63	19
α-helix	68-75	8
α-helix	76-79	4
α-helix	80-83	4
α-helix	94-104	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Trp operon repressor	N, R	protein	107	Escherichia coli	P0A881 (AlphaFold model)

Sequence of entity 1 (N, R), FASTA

>3SSX_1 Trp operon repressor (chains N, R)
AQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLTPDEREALGTRVRIVEEL
LRGEMSQRELKNEFGAGIATITRGSNSLKAAPVELRQWLEEVLLKSD

Ligands and cofactors

ID	Name	Formula	Copies
TAM	Tris(hydroxyethyl)aminomethane	C7 H17 N O3	1

Primary citation

Environment-dependent long-range structural distortion in a temperature-sensitive point mutant. Carey, J., Benoff, B., Harish, B. et al. Protein Sci (2012) 21:63-74. DOI 10.1002/pro.759 · PubMed

Other PDB entries of the same protein (UniProt P0A881 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6ENI 1.1 Å, Tryptophan Repressor TrpR from E.coli variant T44L S88Y with Indole-3-acetic acid as…
6ENN 1.17 Å, Tryptophan Repressor TrpR from E.coli variant T44L T81M N87G S88Y with Indole-3-acetic…
6FAL 1.2 Å, Tryptophan Repressor TrpR from E.coli with 3-Indolepropionic acid as ligand
1JHG 1.3 Å, Trp repressor mutant V58I
6ELG 1.38 Å, Tryptophan Repressor TrpR from E.coli variant M42F T44L T81I S88Y with…
6F9K 1.4 Å, Tryptophan Repressor TrpR from E.coli with 5-methyl-L-tryptophan as ligand
6ELB 1.44 Å, Tryptophan Repressor TrpR from E.coli variant M42F T44L T81M N87G S88Y with…
6EKP 1.46 Å, Tryptophan Repressor TrpR from E.coli variant T44L T81M S88Y with Indole-3-acetic acid…
2OZ9 1.65 Å, E. coli TRP holorepressor, orthorhombic crystal form
6F7G 1.66 Å, Tryptophan Repressor TrpR from E.coli with 5-Methyltryptamine
3SSW 1.67 Å, E. coli trp aporepressor
3WRP 1.8 Å, Flexibility of the DNA-binding domains of trp repressor

Browse structure collections

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