Co-crystal structure of Escherichia coli uracil-DNA glycosylase and a C-terminal fragement of the single-stranded DNA-binding protein. Determined by X-ray diffraction at 1.2 Å resolution. Released 7 Nov 2012.
Explore 3UF7 in 3D Show helices and sheets RCSB PDB PDBe
3UF7 contains 15 α-helices and 6 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 7-11 | 5 | |
| α-helix | 14-16 | 3 | |
| α-helix | 18-33 | 16 | |
| β-strand | 37-38 | 2 | 1 |
| α-helix | 41-43 | 3 | |
| α-helix | 46-50 | 5 | |
| α-helix | 53-55 | 3 | |
| β-strand | 58-62 | 5 | 2 |
| α-helix | 65-66 | 2 | |
| α-helix | 84-86 | 3 | |
| α-helix | 87-99 | 13 | |
| α-helix | 112-115 | 4 | |
| β-strand | 119-123 | 5 | 2 |
| β-strand | 128-129 | 2 | 1 |
| α-helix | 141-155 | 15 | |
| β-strand | 160-164 | 5 | 2 |
| α-helix | 166-171 | 6 | |
| β-strand | 181-185 | 5 | 2 |
| α-helix | 193-195 | 3 | |
| α-helix | 202-212 | 11 | |
| α-helix | 216-218 | 3 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Uracil-DNA glycosylase | A | protein | 237 | Escherichia coli | P12295 (AlphaFold model) |
| Single-stranded DNA-binding protein | B, C | protein | 9 | Escherichia coli | P0AGE0 (AlphaFold model) |
>3UF7_1 Uracil-DNA glycosylase (chains A) MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDVFNAFRFTELGDVKVVI LGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPGFTRPNHGYLESWARQGVL LLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVVFLLWGSHAQKKGAIIDKQRH HVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPIDWMPVLPAESELEHHHHHH
>3UF7_2 Single-stranded DNA-binding protein (chains B, C) MDFDDDIPF
Identification of the SSB-interaction platform of Escherichia coli uracil-DNA glycosylase. George, N.P., Liban, T.J., Reyes-Lamothe, R. et al. To be published.
Other PDB entries of the same protein (UniProt P12295 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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