Single-stranded DNA-binding protein (ssb) is a 178-residue protein from Escherichia coli (strain K12). This is its AlphaFold structure prediction, created 1 Aug 2025. UniProt accession: P0AGE0.
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The mean pLDDT of this model is 80.8 (confident overall). pLDDT is AlphaFold's per-residue confidence score from 0 to 100. In MolViewer, choose the B-factor color scheme to color the model by pLDDT, because AlphaFold stores it in the B-factor column.
| pLDDT band | Meaning | Share of residues |
|---|---|---|
| Above 90 | Very high: backbone and side chains are usually accurate | 58% |
| 70 to 90 | Confident: backbone generally right | 6% |
| 50 to 70 | Low: treat with caution | 25% |
| Below 50 | Very low: often disordered regions | 11% |
What pLDDT means and how to read it
Plays an important role in DNA replication, recombination and repair. Binds to ssDNA and to an array of partner proteins to recruit them to their sites of action during DNA metabolism. Acts as a sliding platform that migrates on DNA via reptation. SSB or its 10 C-terminal amino acids stimulates the ATPase activity of RadD (PubMed:27519413)
Homotetramer. Interacts, via its C-terminus, with many proteins involved in DNA metabolism such as DnaG, HolC, PriA, PriB, PriC, RecO, RecQ and SbcB. Interacts with RadD (PubMed:27519413)
Compare the prediction with experimentally determined structures of the same protein:
| PDB ID | Method | Resolution | Chains and residues |
|---|---|---|---|
| 3UF7 | X-ray | 1.2 Å | B/C=170-178 |
| 8TG8 | X-ray | 1.58 Å | B=170-178 |
| 3SXU | X-ray | 1.85 Å | C=175-178 |
| 4Z0U | X-ray | 2.0 Å | D/E=170-178 |
| 1QVC | X-ray | 2.2 Å | A/B/C/D=2-146 |
| 4MZ9 | X-ray | 2.2 Å | A/B/C/D=1-178 |
| 3C94 | X-ray | 2.7 Å | B/C=170-178 |
| 1EYG | X-ray | 2.8 Å | A/B/C/D=1-116 |
| 1KAW | X-ray | 2.9 Å | A/B/C/D=2-136 |
| 1EQQ | X-ray | 3.2 Å | A/B/C/D=1-178 |
| 1SRU | X-ray | 3.3 Å | A/B/C/D=1-113 |
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