3ZFW: TPR domain of kinesin light chain 2

Crystal structure of the TPR domain of kinesin light chain 2 in complex with a tryptophan-acidic cargo peptide. Determined by X-ray diffraction at 2.9 Å resolution. Released 3 Apr 2013.

Method: X-ray diffraction
Resolution: 2.9 Å
Organisms: MUS MUSCULUS, HOMO SAPIENS
Chains: 4
Atoms: 3,749
Mol. weight: 69.16 kDa
Released: 3 Apr 2013

Explore 3ZFW in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

3ZFW contains 24 α-helices and 0 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	237-252	16
α-helix	256-273	18
α-helix	279-294	16
α-helix	298-315	18
α-helix	321-335	15
α-helix	340-357	18
α-helix	363-378	16
α-helix	382-401	20
α-helix	411-419	9
α-helix	446-461	16
α-helix	465-478	14

Chain B: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	237-252	16
α-helix	256-273	18
α-helix	279-294	16
α-helix	298-315	18
α-helix	321-335	15
α-helix	340-357	18
α-helix	363-378	16
α-helix	382-401	20
α-helix	411-419	9
α-helix	446-461	16
α-helix	465-475	11

Chains X and Y: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	205-208	4

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Kinesin light chain 2	A, B	protein	263	MUS MUSCULUS	O88448 (AlphaFold model)
Pleckstrin homology domain-containing family M member 2	X, Y	protein	45	HOMO SAPIENS	Q8IWE5 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>3ZFW_1 KINESIN LIGHT CHAIN 2 (chains A, B)
VPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKD
HPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQ
GKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAH
EKEFGSVNGENKPIWMHAEEREESKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGA
LYRRQGKLEAAHTLEDCASRSRK

Sequence of entity 2 (X, Y), FASTA

>3ZFW_2 PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY M MEMBER 2 (chains X, Y)
MGSSHHHHHHSSGLVPRGSHMTNLEWDDSAITGSTGSTGSTGSHM

Primary citation

Structural Basis for Kinesin-1:Cargo Recognition. Pernigo, S., Lamprecht, A., Steiner, R.A. et al. Science (2013) 340:356. DOI 10.1126/SCIENCE.1234264 · PubMed

Other PDB entries of the same protein (UniProt O88448 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6EJN 3.2 Å, The KLC2 TPR domain bound to the JIP3 leucine zipper domain
5FJY 4.0 Å, Crystal structure of mouse kinesin light chain 2 (residues 161-480)

Browse structure collections

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