4G1C: Human SIRT5

Human SIRT5 bound to Succ-IDH2 and Carba-NAD. Determined by X-ray diffraction at 1.94 Å resolution. Released 15 Aug 2012.

Method: X-ray diffraction
Resolution: 1.94 Å
Organism: Homo sapiens
Chains: 4
Atoms: 4,502
Mol. weight: 60.13 kDa
Ligands: CNA, ZN
Released: 15 Aug 2012

Explore 4G1C in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4G1C contains 37 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 18 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	37	1	1
α-helix	40-49	10
β-strand	52-57	6	1
α-helix	60-63	4
α-helix	72-75	4
β-strand	76-77	2	2
β-strand	80-81	2	2
α-helix	82-85	4
α-helix	88-93	6
α-helix	95-111	17
α-helix	116-130	15
β-strand	134-139	6	1
α-helix	145-149	5
β-strand	154-156	3	1
β-strand	159-166	8	3
β-strand	172-174	3	3
α-helix	182-184	3
α-helix	201-203	3
β-strand	206	1	4
α-helix	214	1
β-strand	215	1	4
β-strand	216-220	5	3
α-helix	221-222	2
β-strand	226	1	5
α-helix	227-228	2
α-helix	229-241	13
β-strand	244-248	5	1
β-strand	255	1	6
α-helix	257-259	3
α-helix	260-266	7
β-strand	271-275	5	1
α-helix	282-284	3
β-strand	287-290	4	1
α-helix	293-300	8

Chain B: 19 helices, 16 β-strands

Element	Residues	Length	Sheet
β-strand	37	1	7
α-helix	40-49	10
β-strand	52-57	6	7
α-helix	59-62	4
α-helix	63-65	3
β-strand	76-77	2	8
β-strand	80-81	2	8
α-helix	82-85	4
α-helix	88-93	6
α-helix	95-108	14
α-helix	116-130	15
β-strand	134-139	6	7
α-helix	145-149	5
β-strand	154-156	3	7
β-strand	159-166	8	9
β-strand	172-174	3	9
α-helix	182-184	3
α-helix	194-196	3
α-helix	201-203	3
β-strand	206	1	10
α-helix	214	1
β-strand	215	1	10
β-strand	216-220	5	9
α-helix	221-222	2
β-strand	226	1	11
α-helix	227-228	2
α-helix	229-241	13
β-strand	244-248	5	7
β-strand	255	1	12
α-helix	257-259	3
α-helix	260-266	7
β-strand	271-275	5	7
α-helix	282-284	3
β-strand	287-290	4	7
α-helix	293-301	9

Chains D and E: 0 helices, 2 β-strands

Element	Residues	Length	Sheet
β-strand	2	1	5
β-strand	4	1	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
NAD-dependent protein deacylase sirtuin-5, mitochondrial	A, B	protein	267	Homo sapiens	Q9NXA8 (AlphaFold model)
Succinylated IDH2 peptide	D, E	protein	7

Sequence of entity 1 (A, B), FASTA

>4G1C_1 NAD-dependent protein deacylase sirtuin-5, mitochondrial (chains A, B)
PSSSMADFRKFFAKAKHIVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNP
SRVWEFYHYRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLL
EIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGL
LRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFN
TETTPATNRFRFHFQGPCGTTLPEALA

Sequence of entity 2 (D, E), FASTA

>4G1C_2 Succinylated IDH2 peptide (chains D, E)
XAVXCAX

Ligands and cofactors

ID	Name	Formula	Copies
CNA	Carba-nicotinamide-adenine-dinucleotide	C22 H30 N7 O13 P2	1
ZN	Zinc ion	Zn	2

Primary citation

Synthesis of Carba-NAD and the Structures of Its Ternary Complexes with SIRT3 and SIRT5. Szczepankiewicz, B.G., Dai, H., Koppetsch, K.J. et al. J Org Chem (2012) 77:7319-7329. DOI 10.1021/jo301067e · PubMed

Other PDB entries of the same protein (UniProt Q9NXA8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6EQS 1.32 Å, Human Sirt5 in complex with stalled peptidylimidate intermediate of inhibitory compound 29
6LJK 1.39 Å, Crystal structure of human Sirt5 in complex with an internally quenched fluorescent…
8Z54 1.45 Å, Crystal structure of human SIRT5 in complex with succinylated Prx1 fragment
5BWL 1.55 Å, Crystal Structure of SIRT5 in Complex with a Coumarin-Labelled Succinyl Peptide
3RIY 1.55 Å, Sirt5 is an NAD-dependent protein lysine demalonylase and desuccinylase
7X3P 1.56 Å, Crystal structure of human SIRT5 in complex with diazirine inhibitor 9
9V0H 1.67 Å, Crystal structure of human SIRT5 in complex with inhibitor 0904
4F56 1.7 Å, The bicyclic intermediate structure provides insights into the desuccinylation mechanism…
6ACO 1.71 Å, histone lysine desuccinylase Sirt5 in complex with succinyl peptide H2BK120
6LJM 1.78 Å, Crystal structure of human Sirt5 in complex with the fluorogenic tetrapeptide substrate…
6LJN 1.8 Å, Crystal structure of human Sirt5 in complex with the fluorogenic tetrapeptide substrate…
8Z56 1.81 Å, Crystal structure of human Q140L-SIRT5 in complex with succinylated Prx1 fragment

Browse structure collections

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