4I18: Human prolactin receptor

Crystal structure of human prolactin receptor complexed with Fab fragment. Determined by X-ray diffraction at 3.24 Å resolution. Released 20 Nov 2013.

Method: X-ray diffraction
Resolution: 3.24 Å
Organisms: Mus musculus, Homo sapiens
Chains: 6
Atoms: 9,903
Mol. weight: 147.49 kDa
Ligands: CA
Released: 20 Nov 2013

Explore 4I18 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4I18 contains 42 α-helices and 130 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	6-10	5	18
β-strand	13-15	3	19
β-strand	21-28	8	18
α-helix	32-34	3
β-strand	35-42	8	19
β-strand	48-54	7	19
β-strand	61-63	3	19
β-strand	71-76	6	18
β-strand	81-86	6	18
α-helix	91-93	3
β-strand	95-103	9	19
β-strand	105	1	20
β-strand	108	1	20
β-strand	117-118	2	19
β-strand	122-126	5	19
α-helix	130-131	2
β-strand	132	1	21
β-strand	135-139	5	22
β-strand	150-160	11	22
β-strand	161	1	21
β-strand	166-169	4	23
β-strand	174	1	23
β-strand	178-180	3	22
α-helix	181-183	3
β-strand	184-185	2	22
β-strand	191-200	10	22
α-helix	201-203	3
β-strand	210-215	6	23
α-helix	216-218	3
β-strand	220-225	6	23

Chain B: 8 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	5-8	4	13
β-strand	11-15	5	14
β-strand	20-26	7	13
β-strand	34-39	6	14
β-strand	46-50	5	14
β-strand	54-55	2	14
α-helix	56	1
β-strand	63-68	6	13
β-strand	71-76	6	13
α-helix	81-83	3
β-strand	85-91	7	14
β-strand	101	1	14
β-strand	105-110	6	14
β-strand	114	1	15
α-helix	115-116	2
β-strand	117-121	5	16
α-helix	122-124	3
α-helix	125-129	5
β-strand	132-142	11	16
β-strand	143	1	15
β-strand	148-153	6	17
β-strand	156-157	2	17
α-helix	158	1
β-strand	162-166	5	16
α-helix	167-170	4
β-strand	176-185	10	16
α-helix	186-190	5
β-strand	194-200	7	17
β-strand	208-213	6	17

Chain C: 6 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	8-13	6	29
β-strand	14	1	30
β-strand	20-25	6	29
β-strand	35	1	31
β-strand	38-41	4	32
β-strand	49-50	2	32
β-strand	61-64	4	29
α-helix	66-69	4
β-strand	74-79	6	32
β-strand	80-84	5	31
β-strand	86-89	4	31
α-helix	90-92	3
β-strand	93-96	4	32
α-helix	97-100	4
β-strand	101	1	30
α-helix	105-106	2
β-strand	107-113	7	33
β-strand	123-128	6	33
α-helix	129-130	2
β-strand	143-148	6	34
β-strand	150	1	35
β-strand	157-160	4	34
β-strand	166-169	4	33
β-strand	178-180	3	35
β-strand	181-185	5	34
α-helix	190-197	8
β-strand	198-200	3	35

Chain H: 7 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	6-10	5	6
β-strand	13-15	3	7
β-strand	21-28	8	6
α-helix	32-34	3
β-strand	35-42	8	7
β-strand	48-54	7	7
β-strand	61-63	3	7
α-helix	65-67	3
β-strand	71-76	6	6
β-strand	81-86	6	6
β-strand	95-103	9	7
β-strand	105	1	8
β-strand	108	1	8
β-strand	117-118	2	7
β-strand	122-126	5	7
α-helix	129-131	3
β-strand	132	1	9
β-strand	135-139	5	10
β-strand	142	1	11
β-strand	145	1	11
β-strand	150-160	11	10
β-strand	161	1	9
β-strand	166-169	4	12
β-strand	174	1	12
β-strand	178-180	3	10
α-helix	181-183	3
β-strand	184-185	2	10
β-strand	191-200	10	10
α-helix	201-203	3
β-strand	210-215	6	12
α-helix	216-218	3
β-strand	220-225	6	12
α-helix	227-229	3

Chain L: 8 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	5-8	4	1
β-strand	11-15	5	2
β-strand	20-26	7	1
β-strand	34-39	6	2
β-strand	46-50	5	2
β-strand	54-55	2	2
α-helix	56	1
β-strand	63-68	6	1
β-strand	71-76	6	1
α-helix	81-83	3
β-strand	85-91	7	2
β-strand	100-101	2	2
β-strand	105-110	6	2
β-strand	114	1	3
α-helix	115-116	2
β-strand	117-121	5	4
α-helix	122-124	3
α-helix	125-130	6
β-strand	132-142	11	4
β-strand	143	1	3
β-strand	148-153	6	5
β-strand	156-157	2	5
α-helix	158	1
β-strand	162-166	5	4
α-helix	167-170	4
β-strand	176-185	10	4
α-helix	186-190	5
β-strand	194-200	7	5
β-strand	208-213	6	5

Chain R: 7 helices, 17 β-strands

Element	Residues	Length	Sheet
β-strand	8-13	6	24
β-strand	14	1	25
β-strand	20-25	6	24
β-strand	35-42	8	26
β-strand	49-50	2	26
β-strand	61-64	4	24
α-helix	66-68	3
β-strand	75-82	8	26
β-strand	87-88	2	26
β-strand	92-95	4	26
α-helix	98-100	3
β-strand	101	1	25
α-helix	105-106	2
β-strand	107-114	8	27
β-strand	122-128	7	27
α-helix	129-130	2
β-strand	142-150	9	28
β-strand	157-160	4	28
β-strand	166-169	4	27
β-strand	177-186	10	28
α-helix	190-194	5
β-strand	198-201	4	28
α-helix	202-203	2
α-helix	206-208	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
antibody light chain	B, L	protein	217	Mus musculus
antibody heavy chain	A, H	protein	236	Mus musculus
Prolactin receptor	C, R	protein	211	Homo sapiens	P16471 (AlphaFold model)

Sequence of entity 1 (B, L), FASTA

>4I18_1 antibody light chain (chains B, L)
SDIQMTQSPSSLSASVGDRVTITCRASQSVSSAVAWYQQKPGKAPKLLIYSASSLYSGVP
SRFSGSRSGTDFTLTISSLQPEDFATYYCQQYYSYYYPFTFGQGTKVEIKRTVAAPSVFI
FPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSS
TLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC

Sequence of entity 2 (A, H), FASTA

>4I18_2 antibody heavy chain (chains A, H)
EISEVQLVESGGGLVQPGGSLRLSCAASGFNFSSSSMHWVRQAPGQGLEWVAYISPYYGS
TSYADSVKGRFTISADTSKNTAYLQMNSLRAEDTAVYYCARSYGYVYWNAYSSGMDYWGQ
GTLVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHT
FPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHT

Sequence of entity 3 (C, R), FASTA

>4I18_3 Prolactin receptor (chains C, R)
QLPPGKPEIFKCRSPNKETFTCWWRPGTDGGLPTNYSLTYHREGETLMHECPDYITGGPN
SCHFGKQYTSMWRTYIMMVNATNQMGSSFSDELYVDVTYIVQPDPPLELAVEVKQPEDRK
PYLWIKWSPPTLIDLKTGWFTLLYEIRLKPEKAAEWEIHFAGQQTEFKILSLHPGQKYLV
QVRCKPDHGYWSAWSPATFIQIPSDFTMNDT

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	2

Water and common crystallization additives (GOL, ACT) are not listed.

Primary citation

Engineering synthetic antibody binders for allosteric inhibition of prolactin receptor signaling. Rizk, S.S., Kouadio, J.L., Szymborska, A. et al. Cell Commun Signal (2015) 13:1-1. DOI 10.1186/s12964-014-0080-8 · PubMed

Other PDB entries of the same protein (UniProt P16471 (AlphaFold model), which also has an AlphaFold model), best resolution first:

3N06 2.0 Å, A mutant human Prolactin receptor antagonist H27A in complex with the extracellular…
3NCE 2.0 Å, A mutant human Prolactin receptor antagonist H27A in complex with the mutant…
3MZG 2.1 Å, Crystal structure of a human prolactin receptor antagonist in complex with the…
3N0P 2.1 Å, A mutant human Prolactin receptor antagonist H30A in complex with the extracellular…
3NCB 2.1 Å, A mutant human Prolactin receptor antagonist H180A in complex with the extracellular…
3D48 2.5 Å, Crystal structure of a prolactin receptor antagonist bound to the extracellular domain…
3NCC 2.5 Å, A human Prolactin receptor antagonist in complex with the mutant extracellular domain…
3NCF 2.8 Å, A mutant human Prolactin receptor antagonist H30A in complex with the mutant…
1BP3 2.9 Å, The xray structure of a growth hormone-prolactin receptor complex
2LFG Solution structure of the human prolactin receptor ecd domain d2
2N7I NMR structure of the prolactin receptor transmembrane domain

Browse structure collections

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