4NZL: Neutrophil elastase

Extracellular proteins of Staphylococcus aureus inhibit the neutrophil serine proteases. Determined by X-ray diffraction at 1.85 Å resolution. Released 20 Aug 2014.

Method: X-ray diffraction
Resolution: 1.85 Å
Organisms: Homo sapiens, Staphylococcus aureus subsp. aureus
Chains: 2
Atoms: 2,791
Mol. weight: 37.51 kDa
Released: 20 Aug 2014

Explore 4NZL in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4NZL contains 12 α-helices and 31 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 9 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	31	1	1
β-strand	34-35	2	2
α-helix	36-37	2
β-strand	44-49	6	3
β-strand	52-61	10	3
β-strand	64-67	4	3
α-helix	69-72	4
α-helix	77-79	3
β-strand	81-84	4	3
β-strand	88	1	4
β-strand	97-106	10	3
β-strand	110	1	5
β-strand	115	1	5
β-strand	119-123	5	3
α-helix	127-129	3
β-strand	130	1	6
β-strand	133	1	6
α-helix	135-137	3
α-helix	145-146	2
β-strand	150-155	6	2
β-strand	158	1	7
β-strand	165	1	7
β-strand	168	1	4
β-strand	170-177	8	2
β-strand	186-189	4	2
β-strand	196	1	1
β-strand	205-208	4	2
β-strand	211-220	10	2
α-helix	229	1
β-strand	230-234	5	2
α-helix	235-237	3
α-helix	239-246	8

Chain B: 3 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	46-53	8	2
β-strand	56-58	3	2
β-strand	62-65	4	2
β-strand	72-73	2	8
α-helix	74-89	16
α-helix	93-98	6
β-strand	102-108	7	2
β-strand	113-117	5	2
β-strand	127-128	2	8
α-helix	130-132	3
β-strand	133-140	8	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Neutrophil elastase	A	protein	218	Homo sapiens	P08246 (AlphaFold model)
Uncharacterized protein	B	protein	114	Staphylococcus aureus subsp. aureus	A0A0H3K0M1 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>4NZL_1 Neutrophil elastase (chains A)
IVGGRRARPHAWPFMVSLQLRGGHFCGATLIAPNFVMSAAHCVANVNVRAVRVVLGAHNL
SRREPTRQVFAVQRIFENGYDPVNLLNDIVILQLNGSATINANVQVAQLPAQGRRLGNGV
QCLAMGWGLLGRNRGIASVLQELNVTVVTSLCRRSNVCTLVRGRQAGVCFGDSGSPLVCN
GLIHGIASFVRGGCASGLYPDAFAPVAQFVNWIDSIIQ

Sequence of entity 2 (B), FASTA

>4NZL_2 Uncharacterized protein (chains B)
GSTDSNNGYKELTMDGKHTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELAS
QRGVTTEKINNAQTATYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK

Primary citation

Staphylococcus aureus secretes a unique class of neutrophil serine protease inhibitors. Stapels, D.A., Ramyar, K.X., Bischoff, M. et al. Proc Natl Acad Sci U S A (2014) 111:13187-13192. DOI 10.1073/pnas.1407616111 · PubMed

Other PDB entries of the same protein (UniProt P08246 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9SI4 1.14 Å, Structure of human neutrophil elastase in complex with a…
8VK5 1.56 Å, Human Sputum Leucocyte Elastase (uncomplexed)
5ABW 1.6 Å, Neutrophil elastase inhibitors for the treatment of (cardio)pulmonary diseases
4WVP 1.63 Å, Crystal structure of an activity-based probe HNE complex
2Z7F 1.7 Å, Crystal structure of the complex of human neutrophil elastase with 1/2SLPI
9ASS 1.75 Å, Crystal Structure of Neutrophil Elastase Inhibited by Eap4 from S. aureus
5A0A 1.78 Å, Crystal Structure of human neutrophil elastase in complex with a dihydropyrimidone…
1PPF 1.8 Å, X-ray crystal structure of the complex of human leukocyte elastase (pmn elastase) and…
2RG3 1.8 Å, Covalent complex structure of elastase
8G25 1.8 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…
5A09 1.81 Å, Crystal Structure of human neutrophil elastase in complex with a dihydropyrimidone…
8G24 1.82 Å, Crystal Structure of Cathepsin-G and Neutrophil Elastase Inhibited by S. aureus EapH2 at…

Browse structure collections

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