4PWX: ATPase GET3

Crystal structure of an ATP-bound Get3-Get4-Get5 complex from S.cerevisiae. Determined by X-ray diffraction at 5.4 Å resolution. Released 9 Apr 2014.

Method: X-ray diffraction
Resolution: 5.4 Å
Organism: Saccharomyces cerevisiae
Chains: 6
Atoms: 10,159
Mol. weight: 159.9 kDa
Ligands: ZN, MG, ATP
Released: 9 Apr 2014

Explore 4PWX in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4PWX contains 64 α-helices and 20 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 14 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	10-13	4
β-strand	20-24	5	1
α-helix	31-45	15
β-strand	51-54	4	1
α-helix	62-66	5
β-strand	75-76	2	1
α-helix	77	1
β-strand	83-85	3	1
α-helix	90-96	7
α-helix	127-130	4
α-helix	136-154	19
β-strand	162-165	4	1
α-helix	170-177	8
α-helix	179-188	10
α-helix	213-230	18
β-strand	236-243	8	1
α-helix	246-261	16
β-strand	265-274	10	1
α-helix	286-305	20
β-strand	311-315	5	1
α-helix	323-335	13
α-helix	346-348	3

Chain B: 16 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	10-13	4
β-strand	20-24	5	2
α-helix	31-45	15
β-strand	51-54	4	2
α-helix	62-66	5
β-strand	75-76	2	2
α-helix	77	1
β-strand	83-85	3	2
α-helix	90-96	7
α-helix	127-130	4
α-helix	136-154	19
β-strand	162-166	5	2
α-helix	170-177	8
α-helix	179-188	10
α-helix	212-230	19
β-strand	236-243	8	2
α-helix	246-261	16
β-strand	265-274	10	2
α-helix	286-305	20
β-strand	311-315	5	2
α-helix	323-331	9
α-helix	332-334	3
α-helix	340-343	4
α-helix	346-348	3

Chain C: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	17-25	9
α-helix	29-45	17
α-helix	49-65	17
α-helix	69-85	17
α-helix	92-104	13
α-helix	112-125	14
α-helix	134-146	13
α-helix	150-158	9
α-helix	162-178	17
α-helix	184-200	17
α-helix	204-222	19
β-strand	226-231	6	4
β-strand	234-239	6	4
α-helix	243-257	15
α-helix	260-269	10
α-helix	271-276	6
α-helix	278-287	10

Chains D and F: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	7-19	13
α-helix	44-48	5

Chain E: 15 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	15-24	10
α-helix	29-45	17
α-helix	49-65	17
α-helix	69-85	17
α-helix	92-103	12
α-helix	112-125	14
α-helix	134-146	13
α-helix	150-158	9
α-helix	162-178	17
α-helix	184-200	17
α-helix	204-222	19
β-strand	226-231	6	3
β-strand	234-239	6	3
α-helix	243-257	15
α-helix	260-269	10
α-helix	271-276	6
α-helix	278-287	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
ATPase GET3	A, B	protein	356	Saccharomyces cerevisiae	Q12154 (AlphaFold model)
Golgi to ER traffic protein 4	C, E	protein	288	Saccharomyces cerevisiae	Q12125 (AlphaFold model)
Ubiquitin-like protein MDY2	D, F	protein	54	Saccharomyces cerevisiae	Q12285 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>4PWX_1 ATPase GET3 (chains A, B)
SVDDLTVEPNLHSLITSTTHKWIFVGGKGGVGKTTSSCSIAIQMALSQPNKQFLLISTVP
AHNLSDAFGEKFGKDARKVTGMNNLSCMEIDPSAALKDMNDMAVSRANNNGSDGQGDDLG
SLLQGGALADLTGSIPGIDEALSFMEVMKHIKRQEQGEGETFDTVIFDTAPTGHTLRFLQ
LPNTLSKLLEKFGEITNKLGPMLNSFMGAGNVDISGKLNELKANVETIRQQFTDPDLTTF
VCVCISEFLSLYETERLIQELISYDMDVNSIIVNQLLFAENDQEHNCKRCQARWKMQKKY
LDQIDELYEDFHVVKMPLCAGEIRGLNNLTKFSQFLNKEYNPITDGKVIYELEDKE

Sequence of entity 2 (C, E), FASTA

>4PWX_2 Golgi to ER traffic protein 4 (chains C, E)
MGAKLAKTLQRFENKIKAGDYYEAHQTLRTIANRYVRSKSYEHAIELISQGALSFLKAKQ
GGSGTDLIFYLLEVYDLAEVKVDDISVARLVRLIAELDPSEPNLKDVITGMNNWSIKFSE
YKFGDPYLHNTIGSKLLEGDFVYEAERYFMLGTHDSMIKYVDLLWDWLCQVDDIEDSTVA
EFFSRLVFNYLFISNISFAHESKDIFLERFIEKFHPKYEKIDKNGYEIVFFEDYSDLNFL
QLLLITCQTADASYFLNLKNHYLDFSQAYKSELEFLGQEYFNENLYFQ

Sequence of entity 3 (D, F), FASTA

>4PWX_3 Ubiquitin-like protein MDY2 (chains D, F)
MSTSASGPEHEFVSKFLTLATLTEPKLPKSYTKPLKDVTNLGVPLPTLKYKYKQ

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1
MG	Magnesium ion	Mg	2
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	2

Primary citation

Crystal structure of ATP-bound Get3-Get4-Get5 complex reveals regulation of Get3 by Get4. Gristick, H.B., Rao, M., Chartron, J.W. et al. Nat Struct Mol Biol (2014) 21:437-442. DOI 10.1038/nsmb.2813 · PubMed

Other PDB entries of the same protein (UniProt Q12154 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2WOJ 1.99 Å, ADP-AlF4 complex of S. cerevisiae GET3
4XTR 2.05 Å, Structure of Get3 bound to the transmembrane domain of Pep12
3ZS9 2.1 Å, S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment
3H84 2.3 Å, Crystal structure of GET3
4XVU 2.35 Å, Structure of Get3 bound to the transmembrane domain of Nyv1
4XWO 2.75 Å, Structure of Get3 bound to the transmembrane domain of Sec22
3A36 2.8 Å, Structural insight into the membrane insertion of tail-anchored proteins by Get3
5BW8 2.8 Å, 2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae
3A37 3.0 Å, Structural insight into the membrane insertion of tail-anchored proteins by Get3
3B2E 3.0 Å, Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1…
3SJA 3.0 Å, Crystal structure of S. cerevisiae Get3 in the open state in complex with Get1 cytosolic…
3ZS8 3.0 Å, S. cerevisiae Get3 complexed with a cytosolic Get1 fragment

Browse structure collections

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