5BW8: ATPase GET3

2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae. Determined by X-ray diffraction at 2.8 Å resolution. Released 14 Oct 2015.

Method: X-ray diffraction
Resolution: 2.8 Å
Organisms: Saccharomyces cerevisiae (strain RM11-1a), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae
Chains: 5
Atoms: 7,742
Mol. weight: 126.18 kDa
Ligands: ZN
Released: 14 Oct 2015

Explore 5BW8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5BW8 contains 53 α-helices and 20 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	10-13	4
β-strand	20-24	5	1
α-helix	31-45	15
β-strand	51-55	5	1
α-helix	61-66	6
β-strand	75-76	2	1
α-helix	77	1
β-strand	83-87	5	1
α-helix	90-96	7
α-helix	128-132	5
α-helix	136-154	19
β-strand	162-166	5	1
α-helix	175-178	4
α-helix	179-194	16
α-helix	200-205	6
α-helix	209-230	22
β-strand	236-243	8	1
α-helix	246-261	16
β-strand	268-274	7	1
α-helix	277-279	3
α-helix	286-305	20
β-strand	310-315	6	1
α-helix	320-321	2
α-helix	324-335	12
α-helix	340-351	12

Chain B: 15 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	10-13	4
β-strand	20-24	5	2
α-helix	31-45	15
β-strand	51-55	5	2
α-helix	61-66	6
β-strand	75-76	2	2
α-helix	77	1
β-strand	83-87	5	2
α-helix	90-99	10
α-helix	136-154	19
β-strand	162-166	5	2
α-helix	175-178	4
α-helix	179-189	11
α-helix	213-230	18
β-strand	236-243	8	2
α-helix	246-261	16
β-strand	268-274	7	2
α-helix	277-280	4
α-helix	286-305	20
β-strand	310-315	6	2
α-helix	320-321	2
α-helix	324-335	12
α-helix	340-353	14

Chain C: 16 helices, 3 β-strands

Element	Residues	Length	Sheet
α-helix	12-25	14
α-helix	29-45	17
α-helix	49-65	17
α-helix	69-85	17
α-helix	92-104	13
α-helix	112-125	14
α-helix	134-146	13
α-helix	150-158	9
α-helix	162-178	17
α-helix	185-200	16
β-strand	203	1	3
α-helix	204-222	19
β-strand	226-231	6	4
β-strand	234-239	6	4
α-helix	243-257	15
α-helix	260-269	10
α-helix	271-276	6
α-helix	278-284	7
α-helix	285-289	5

Chain D: 4 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	4-6	3
α-helix	7-20	14
β-strand	32	1	3
α-helix	35-37	3
α-helix	44-48	5

Chain Z: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	6-12	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
ATPase GET3	A, B	protein	373	Saccharomyces cerevisiae (strain RM11-1a)	Q12154 (AlphaFold model)
Golgi to ER traffic protein 4	C	protein	305	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q12125 (AlphaFold model)
Ubiquitin-like protein MDY2	D	protein	54	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	Q12285 (AlphaFold model)
Unknown Protein	Z	protein	13	Saccharomyces cerevisiae

Sequence of entity 1 (A, B), FASTA

>5BW8_1 ATPase GET3 (chains A, B)
MGGSHHHHHHGENLYFQSVDDLTVEPNLHSLITSTTHKWIFVGGKGGVGKTTSSCSIAIQ
MALSQPNKQFLLISTDPAHNLSDAFGEKFGKDARKVTGMNNLSCMEIDPSAALKDMNDMA
VSRANNNGSDGQGDDLGSLLQGGALADLTGSIPGIDEALSFMEVMKHIKRQEQGEGETFD
TVIFDTAPTGHTLRFLQLPNTLSKLLEKFGEITNKLGPMLNSFMGAGNVDISGKLNELKA
NVETIRQQFTDPDLTTFVCVCISEFLSLYETERLIQELISYDMDVNSIIVNQLLFAENDQ
EHNCKRCQARWKMQKKYLDQIDELYEDFHVVKMPLCAGEIRGLNNLTKFSQFLNKEYNPI
TDGKVIYELEDKE

Sequence of entity 2 (C), FASTA

>5BW8_2 Golgi to ER traffic protein 4 (chains C)
MVPAESNAVQAKLAKTLQRFENKIKAGDYYEAHQTLRTIANRYVRSKSYEHAIELISQGA
LSFLKAKQGGSGTDLIFYLLEVYDLAEVKVDDISVARLVRLIAELDPSEPNLKDVITGMN
NWSIKFSEYKFGDPYLHNTIGSKLLEGDFVYEAERYFMLGTHDSMIKYVDLLWDWLCQVD
DIEDSTVAEFFSRLVFNYLFISNISFAHESKDIFLERFIEKFHPKYEKIDKNGYEIVFFE
DYSDLNFLQLLLITCQTADASYFLNLKNHYLDFSQAYKSELEFLGQEYFNENLYFQSLEH
HHHHH

Sequence of entity 3 (D), FASTA

>5BW8_3 Ubiquitin-like protein MDY2 (chains D)
MSTSASGPEHEFVSKFLTLATLTEPKLPKSYTKPLKDVTNLGVPLPTLKYKYKQ

Sequence of entity 4 (Z), FASTA

>5BW8_4 Unknown Protein (chains Z)
XXXXXXXXXXXXX

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1

Primary citation

Mechanism of Assembly of a Substrate Transfer Complex during Tail-anchored Protein Targeting. Gristick, H.B., Rome, M.E., Chartron, J.W. et al. J Biol Chem (2015) 290:30006-30017. DOI 10.1074/jbc.M115.677328 · PubMed

Other PDB entries of the same protein (UniProt Q12154 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2WOJ 1.99 Å, ADP-AlF4 complex of S. cerevisiae GET3
4XTR 2.05 Å, Structure of Get3 bound to the transmembrane domain of Pep12
3ZS9 2.1 Å, S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment
3H84 2.3 Å, Crystal structure of GET3
4XVU 2.35 Å, Structure of Get3 bound to the transmembrane domain of Nyv1
4XWO 2.75 Å, Structure of Get3 bound to the transmembrane domain of Sec22
3A36 2.8 Å, Structural insight into the membrane insertion of tail-anchored proteins by Get3
3A37 3.0 Å, Structural insight into the membrane insertion of tail-anchored proteins by Get3
3B2E 3.0 Å, Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1…
3SJA 3.0 Å, Crystal structure of S. cerevisiae Get3 in the open state in complex with Get1 cytosolic…
3ZS8 3.0 Å, S. cerevisiae Get3 complexed with a cytosolic Get1 fragment
9NS5 3.19 Å, Get3(D57N)-Get4/5 Complex (ATP-bound)

Browse structure collections

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