4RR2: Human primase

Crystal structure of human primase. Determined by X-ray diffraction at 2.65 Å resolution. Released 21 Jan 2015.

Method

X-ray diffraction

Resolution

2.65 Å

Organism

Homo sapiens

Chains

4

Atoms

11,896

Mol. weight

218.23 kDa

Ligands

ZN, SF4

Released

21 Jan 2015

Explore 4RR2 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4RR2 contains 91 α-helices and 34 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 27 helices, 13 β-strands

Molecules and chains

>4RR2_1 DNA primase small subunit (chains A, C)
METFDPTELPELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSF
NNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRR
CCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSA
VRSGIVEYLSLVKGGQDVKKKVHLSEKIHPFIRKSINIIKKYFEEYALVNQDILENKESW
DKILALVPETIHDELQQSFQKSHNSLQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQY
CFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICRELDAI
STNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENLDKSRKGELLKKSDLQKDF

>4RR2_2 DNA primase large subunit (chains B, D)
MEFSGRKWRKLRLAGDQRNASYPHCLQFYLQPPSENISLIEFENLAIDRVKLLKSVENLG
VSYVKGTEQYQSKLESELRKLKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEELRRWF
IQQEMDLLRFRFSILPKDKIQDFLKDSQLQFEAISDEEKTLREQEIVASSPSLSGLKLGF
ESIYKIPFADALDLFRGRKVYLEDGFAYVPLKDIVAIILNEFRAKLSKALALTARSLPAV
QSDERLQPLLNHLSHSYTGQDYSTQGNVGKISLDQIDLLSTKSFPPCMRQLHKALRENHH
LRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKGKMDPDKFDKGYSYNIRHSFGKEGKRT
DYTPFSCLKIILSNPPSQGDYHGCPFRHSDPELLKQKLQSYKISPGGISQILDLVKGTHY
QVACQKYFEMIHNVDDCGFSLNHPNQFFCESQRILNGGKDIKKEPIQPETPQPKPSVQKT
KDASSALASLNSSLEMDMEGLEDYFSEDS

Ligands and cofactors

Primary citation

Crystal structure of the human primase. Baranovskiy, A.G., Zhang, Y., Suwa, Y. et al. J Biol Chem (2015) 290:5635-5646. DOI 10.1074/jbc.M114.624742 · PubMed

Related structures

Other PDB entries of the same protein (UniProt P49642 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 6RB4 1.5 Å, Crystal structure of the Pri1 subunit of human primase
• 4LIK 1.7 Å, Crystal structure of the catalytic subunit of human primase
• 6R5E 1.85 Å, Crystal structure of the Pri1 subunit of human primase bound to 2F-ATP
• 6R5D 1.95 Å, Crystal structure of the Pri1 subunit of human primase bound to dATP
• 4MHQ 2.2 Å, Crystal structure of human primase catalytic subunit
• 6R4U 2.2 Å, Crystal structure of the Pri1 subunit of human primase bound to fludarabine triphosphate
• 6R4T 2.35 Å, Crystal structure of the Pri1 subunit of human primase bound to vidarabine triphosphate
• 4LIL 2.6 Å, Crystal structure of the catalytic subunit of human primase bound to UTP and Mn
• 4BPU 2.7 Å, Crystal structure of human primase in heterodimeric form, comprising PriS and truncated…
• 6R4S 2.75 Å, Crystal structure of the Pri1 subunit of human primase bound to ATP
• 8VY3 2.98 Å, Human DNA polymerase alpha/primase - AavLEA1 (1:40 molar ratio)
• 4BPW 3.0 Å, Crystal structure of human primase bound to UTP

Browse structure collections

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