4WZM: RNA dependent RNA polymerase

Mutant K18E of RNA dependent RNA polymerase from Foot-and-Mouth Disease Virus complexed with RNA. Determined by X-ray diffraction at 2.52 Å resolution. Released 6 May 2015.

Method: X-ray diffraction
Resolution: 2.52 Å
Organisms: Foot-and-mouth disease virus, synthetic construct
Chains: 3
Atoms: 4,112
Mol. weight: 59.16 kDa
Ligands: MN
Released: 6 May 2015

Explore 4WZM in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

4WZM contains 27 α-helices and 25 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 27 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	2-8	7	1
α-helix	12-14	3
β-strand	21-23	3	2
α-helix	25-30	6
β-strand	34-36	3	3
α-helix	37-38	2
α-helix	47	1
α-helix	52-56	5
α-helix	68-89	22
α-helix	98-103	6
β-strand	105	1	4
β-strand	108	1	4
α-helix	109-112	4
α-helix	128-131	4
β-strand	133	1	5
β-strand	138	1	5
α-helix	143-151	9
β-strand	159-163	5	1
β-strand	167-169	3	3
α-helix	170-174	5
β-strand	180-183	4	1
α-helix	184-185	2
α-helix	186-205	20
β-strand	208	1	6
β-strand	213	1	6
α-helix	219-230	12
β-strand	235-238	4	6
β-strand	239-241	3	7
α-helix	250-260	11
α-helix	263-265	3
α-helix	270-276	7
β-strand	280-285	6	1
β-strand	288-293	6	1
α-helix	303-322	20
α-helix	328-330	3
β-strand	332-336	5	6
β-strand	339-344	6	6
α-helix	350-358	9
β-strand	364-366	3	7
β-strand	384-385	2	8
β-strand	388-392	5	8
β-strand	399-402	4	8
α-helix	405-413	9
β-strand	414-416	3	2
α-helix	420-431	12
α-helix	432-434	3
α-helix	436-443	8
α-helix	444-446	3
β-strand	447	1	9
β-strand	450	1	9
α-helix	455-466	12
α-helix	468-474	7

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
RNA dependent RNA polymerase	A	protein	481	Foot-and-mouth disease virus	P03311
RNA template	B	RNA	8	synthetic construct
RNA primer	C	RNA	7	synthetic construct

Sequence of entity 1 (A), FASTA

>4WZM_1 RNA dependent RNA polymerase (chains A)
GLIVDTRDVEERVHVMRETKLAPTVAHGVFNPEFGPAALSNKDPRLNEGVVLDEVIFSKH
KGDTKMSAEDKALFRRCAADYASRLHSVLGTANAPLSIYEAIKGVDGLDAMEPDTAPGLP
WALQGKRRGALIDFENGTVGPEVEAALKLMEKREYKFACQTFLKDEIRPMEKVRAGKTRI
VDVLPVEHILYTRMMIGRFCAQMHSNNGPQIGSAVGCNPDVDWQRFGTHFAQYRNVWDVD
YSAFDANHCSDAMNIMFEEVFRTEFGFHPNAEWILKTLVNTEHAYENKRITVEGGMPSGC
SATSIINTILNNIYVLYALRRHYEGVELDTYTMISYGDDIVVASDYDLDFEALKPHFKSL
GQTITPADKSDKGFVLGHSITDVTFLKRHFHMDYGTGFYKPVMASKTLEAILSFARRGTI
QEKLISVAGLAVHSGPDEYRRLFEPFQGLFEIPSYRSLYLRWVNAVCGDAAAALEHHHHH
H

Sequence of entity 2 (B), FASTA

>4WZM_2 RNA template (chains B)
AUGGGCCC

Sequence of entity 3 (C), FASTA

>4WZM_3 RNA primer (chains C)
UGGGCCC

Ligands and cofactors

ID	Name	Formula	Copies
MN	Manganese (II) ion	Mn	1

Water and common crystallization additives (GOL, ACT) are not listed.

Primary citation

Multifunctionality of a picornavirus polymerase domain: nuclear localization signal and nucleotide recognition. Ferrer-Orta, C., de la Higuera, I., Caridi, F. et al. J Virol (2015) 89:6848-6859. DOI 10.1128/JVI.03283-14 · PubMed

Other PDB entries of the same protein (UniProt P03311), best resolution first:

8C1N 1.7 Å, FMDV 3D polymerase in complex with 3B1 protein solved in P212121 space group
4WYW 1.8 Å, Mutant K20E of 3D polymerase from Foot-and-Mouth Disease Virus
6KWL 1.8 Å, Crystal structure of pSLA-1*0401(R156A) complex with FMDV-derived epitope MTAHITVPY
8C2P 1.85 Å, FMDV 3D polymerase in complex with 3B3
4WYL 2.0 Å, Mutant K18E of 3D polymerase from Foot-and-Moth Disease Virus
6GVY 2.2 Å, Mutant M16A of RNA dependent RNA polymerase 3D from Foot-and-Mouth disease Virus…
6S2L 2.3 Å, FMDV 3D polymerase crystallized in presence of (F)uridylylated VPg peptide
6GVV 2.35 Å, Mutant M16A of RNA dependent RNA polymerase 3D from Foot-and-Mouth disease Virus
5N8X 2.4 Å, Trigonal structure of mutant V173I of 3D polymerase from Foot-and-Mouth Disease Virus
6KWK 2.5 Å, Crystal structure of pSLA-1*0401 complex with FMDV-derived epitope MTAHITVPY
9I3H 2.5 Å, CP of RHDV mutant - N15
5N95 2.6 Å, Tetragonal structure of mutant V173I of 3D polymerase from Foot-and-Mouth Disease Virus

Browse structure collections

About this viewer

MolViewer shows 4WZM directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.