9NS5: Get3(D57N)-Get4/5 Complex

Get3(D57N)-Get4/5 Complex (ATP-bound). Determined by electron microscopy at 3.19 Å resolution. Released 24 Sept 2025.

Method
Electron microscopy
Resolution
3.19 Å
Organism
Saccharomyces cerevisiae S288C
Chains
6
Atoms
10,901
Mol. weight
209.75 kDa
Ligands
ZN, ATP, MG
Released
24 Sept 2025

Explore 9NS5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

9NS5 contains 62 α-helices and 20 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and D: 11 helices, 8 β-strands

ElementResiduesLengthSheet
α-helix10-134
β-strand20-2451
α-helix31-4515
β-strand51-5551
α-helix62-665
β-strand75-7621
α-helix771
β-strand83-8751
α-helix128-1314
α-helix136-15419
β-strand162-16651
α-helix170-20435
α-helix212-23019
β-strand236-24381
α-helix246-26217
β-strand266-27491
α-helix286-30520
β-strand310-31561
α-helix324-33512
Chains B and E: 17 helices, 2 β-strands
ElementResiduesLengthSheet
α-helix14-2613
α-helix29-4517
α-helix49-6517
α-helix69-8618
α-helix92-10413
α-helix112-12514
α-helix134-14613
α-helix150-1578
α-helix162-17817
α-helix186-20015
α-helix204-22118
β-strand226-23162
β-strand234-23962
α-helix243-25715
α-helix260-26910
α-helix271-2766
α-helix278-28811
α-helix293-2964
α-helix299-3068
Chains C and F: 3 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix8-2013
α-helix35-373
α-helix44-485

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Ubiquitin-like protein MDY2C, Fprotein212Saccharomyces cerevisiae S288CQ12285 (AlphaFold model)
ATPase GET3A, Dprotein376Saccharomyces cerevisiae S288CQ12154 (AlphaFold model)
Golgi to ER traffic protein 4B, Eprotein334Saccharomyces cerevisiae S288CQ12125 (AlphaFold model)
Sequence of entity 1 (C, F), FASTA
>9NS5_1 Ubiquitin-like protein MDY2 (chains C, F)
MSTSASGPEHEFVSKFLTLATLTEPKLPKSYTKPLKDVTNLGVPLPTLKYKYKQNRAKKL
KLHQDQQGQDNAAVHLTLKKIQAPKFSIEHDFSPSDTILQIKQHLISEEKASHISEIKLL
LKGKVLHDNLFLSDLKVTPANSTITVMIKPNPTISKEPEAEKSTNSPAPAPPQELTVPWD
DIEALLKNNFENDQAAVRQVMERLQKGWSLAK
Sequence of entity 2 (A, D), FASTA
>9NS5_2 ATPase GET3 (chains A, D)
MGSSHHHHHHSSGENLYFQGHMMDLTVEPNLHSLITSTTHKWIFVGGKGGVGKTTSSCSI
AIQMALSQPNKQFLLISTNPAHNLSDAFGEKFGKDARKVTGMNNLSCMEIDPSAALKDMN
DMAVSRANNNGSDGQGDDLGSLLQGGALADLTGSIPGIDEALSFMEVMKHIKRQEQGEGE
TFDTVIFDTAPTGHTLRFLQLPNTLSKLLEKFGEITNKLGPMLNSFMGAGNVDISGKLNE
LKANVETIRQQFTDPDLTTFVCVCISEFLSLYETERLIQELISYDMDVNSIIVNQLLFAE
NDQEHNCKRCQARWKMQKKYLDQIDELYEDFHVVKMPLCAGEIRGLNNLTKFSQFLNKEY
NPITDGKVIYELEDKE
Sequence of entity 3 (B, E), FASTA
>9NS5_3 Golgi to ER traffic protein 4 (chains B, E)
MVPAESNAVQAKLAKTLQRFENKIKAGDYYEAHQTLRTIANRYVRSKSYEHAIELISQGA
LSFLKAKQGGSGTDLIFYLLEVYDLAEVKVDDISVARLVRLIAELDPSEPNLKDVITGMN
NWSIKFSEYKFGDPYLHNTIGSKLLEGDFVYEAERYFMLGTHDSMIKYVDLLWDWLCQVD
DIEDSTVAEFFSRLVFNYLFISNISFAHESKDIFLERFIEKFHPKYEKIDKNGYEIVFFE
DYSDLNFLQLLLITCQTKDKSYFLNLKNHYLDFSQAYKSELEFLGQEYFNIVAPKQTNFL
QDMMSGFLGGSKSGGGGGENLYFQGGSSHHHHHH

Ligands and cofactors

IDNameFormulaCopies
ZNZinc ionZn1
ATPAdenosine-5'-triphosphateC10 H16 N5 O13 P32
MGMagnesium ionMg2

Primary citation

Get4/5-mediated remodeling of Get3's substrate-binding chamber: Insights into tail-anchored protein targeting by the GET pathway. Granados-Villanueva, D., Rossow, A., Kim, K.H. J Biol Chem (2025) 301:110667-110667. DOI 10.1016/j.jbc.2025.110667 · PubMed

Other PDB entries of the same protein (UniProt Q12285 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 9NS5 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.