5BW8: ATPase GET3
2.8 A crystal structure of a Get3-Get4-Get5 intermediate complex from S.cerevisiae. Determined by X-ray diffraction at 2.8 Å resolution. Released 14 Oct 2015.
- Method
- X-ray diffraction
- Resolution
- 2.8 Å
- Organisms
- Saccharomyces cerevisiae (strain RM11-1a), Saccharomyces cerevisiae (strain ATCC 204508 / S288c), Saccharomyces cerevisiae
- Chains
- 5
- Atoms
- 7,742
- Mol. weight
- 126.18 kDa
- Ligands
- ZN
- Released
- 14 Oct 2015
Explore 5BW8 in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
5BW8 contains 53 α-helices and 20 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 17 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-13 | 4 | |
| β-strand | 20-24 | 5 | 1 |
| α-helix | 31-45 | 15 | |
| β-strand | 51-55 | 5 | 1 |
| α-helix | 61-66 | 6 | |
| β-strand | 75-76 | 2 | 1 |
| α-helix | 77 | 1 | |
| β-strand | 83-87 | 5 | 1 |
| α-helix | 90-96 | 7 | |
| α-helix | 128-132 | 5 | |
| α-helix | 136-154 | 19 | |
| β-strand | 162-166 | 5 | 1 |
| α-helix | 175-178 | 4 | |
| α-helix | 179-194 | 16 | |
| α-helix | 200-205 | 6 | |
| α-helix | 209-230 | 22 | |
| β-strand | 236-243 | 8 | 1 |
| α-helix | 246-261 | 16 | |
| β-strand | 268-274 | 7 | 1 |
| α-helix | 277-279 | 3 | |
| α-helix | 286-305 | 20 | |
| β-strand | 310-315 | 6 | 1 |
| α-helix | 320-321 | 2 | |
| α-helix | 324-335 | 12 | |
| α-helix | 340-351 | 12 | |
Chain B: 15 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 10-13 | 4 | |
| β-strand | 20-24 | 5 | 2 |
| α-helix | 31-45 | 15 | |
| β-strand | 51-55 | 5 | 2 |
| α-helix | 61-66 | 6 | |
| β-strand | 75-76 | 2 | 2 |
| α-helix | 77 | 1 | |
| β-strand | 83-87 | 5 | 2 |
| α-helix | 90-99 | 10 | |
| α-helix | 136-154 | 19 | |
| β-strand | 162-166 | 5 | 2 |
| α-helix | 175-178 | 4 | |
| α-helix | 179-189 | 11 | |
| α-helix | 213-230 | 18 | |
| β-strand | 236-243 | 8 | 2 |
| α-helix | 246-261 | 16 | |
| β-strand | 268-274 | 7 | 2 |
| α-helix | 277-280 | 4 | |
| α-helix | 286-305 | 20 | |
| β-strand | 310-315 | 6 | 2 |
| α-helix | 320-321 | 2 | |
| α-helix | 324-335 | 12 | |
| α-helix | 340-353 | 14 | |
Chain C: 16 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 12-25 | 14 | |
| α-helix | 29-45 | 17 | |
| α-helix | 49-65 | 17 | |
| α-helix | 69-85 | 17 | |
| α-helix | 92-104 | 13 | |
| α-helix | 112-125 | 14 | |
| α-helix | 134-146 | 13 | |
| α-helix | 150-158 | 9 | |
| α-helix | 162-178 | 17 | |
| α-helix | 185-200 | 16 | |
| β-strand | 203 | 1 | 3 |
| α-helix | 204-222 | 19 | |
| β-strand | 226-231 | 6 | 4 |
| β-strand | 234-239 | 6 | 4 |
| α-helix | 243-257 | 15 | |
| α-helix | 260-269 | 10 | |
| α-helix | 271-276 | 6 | |
| α-helix | 278-284 | 7 | |
| α-helix | 285-289 | 5 | |
Chain D: 4 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 4-6 | 3 | |
| α-helix | 7-20 | 14 | |
| β-strand | 32 | 1 | 3 |
| α-helix | 35-37 | 3 | |
| α-helix | 44-48 | 5 | |
Chain Z: 1 helix, 0 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 6-12 | 7 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| ATPase GET3 | A, B | protein | 373 | Saccharomyces cerevisiae (strain RM11-1a) | Q12154 (AlphaFold model) |
| Golgi to ER traffic protein 4 | C | protein | 305 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Q12125 (AlphaFold model) |
| Ubiquitin-like protein MDY2 | D | protein | 54 | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) | Q12285 (AlphaFold model) |
| Unknown Protein | Z | protein | 13 | Saccharomyces cerevisiae | |
Sequence of entity 1 (A, B), FASTA
>5BW8_1 ATPase GET3 (chains A, B)
MGGSHHHHHHGENLYFQSVDDLTVEPNLHSLITSTTHKWIFVGGKGGVGKTTSSCSIAIQ
MALSQPNKQFLLISTDPAHNLSDAFGEKFGKDARKVTGMNNLSCMEIDPSAALKDMNDMA
VSRANNNGSDGQGDDLGSLLQGGALADLTGSIPGIDEALSFMEVMKHIKRQEQGEGETFD
TVIFDTAPTGHTLRFLQLPNTLSKLLEKFGEITNKLGPMLNSFMGAGNVDISGKLNELKA
NVETIRQQFTDPDLTTFVCVCISEFLSLYETERLIQELISYDMDVNSIIVNQLLFAENDQ
EHNCKRCQARWKMQKKYLDQIDELYEDFHVVKMPLCAGEIRGLNNLTKFSQFLNKEYNPI
TDGKVIYELEDKE
Sequence of entity 2 (C), FASTA
>5BW8_2 Golgi to ER traffic protein 4 (chains C)
MVPAESNAVQAKLAKTLQRFENKIKAGDYYEAHQTLRTIANRYVRSKSYEHAIELISQGA
LSFLKAKQGGSGTDLIFYLLEVYDLAEVKVDDISVARLVRLIAELDPSEPNLKDVITGMN
NWSIKFSEYKFGDPYLHNTIGSKLLEGDFVYEAERYFMLGTHDSMIKYVDLLWDWLCQVD
DIEDSTVAEFFSRLVFNYLFISNISFAHESKDIFLERFIEKFHPKYEKIDKNGYEIVFFE
DYSDLNFLQLLLITCQTADASYFLNLKNHYLDFSQAYKSELEFLGQEYFNENLYFQSLEH
HHHHH
Sequence of entity 3 (D), FASTA
>5BW8_3 Ubiquitin-like protein MDY2 (chains D)
MSTSASGPEHEFVSKFLTLATLTEPKLPKSYTKPLKDVTNLGVPLPTLKYKYKQ
Sequence of entity 4 (Z), FASTA
>5BW8_4 Unknown Protein (chains Z)
XXXXXXXXXXXXX
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ZN | Zinc ion | Zn | 1 |
Primary citation
Mechanism of Assembly of a Substrate Transfer Complex during Tail-anchored Protein Targeting. Gristick, H.B., Rome, M.E., Chartron, J.W. et al. J Biol Chem (2015) 290:30006-30017. DOI 10.1074/jbc.M115.677328 · PubMed
Other PDB entries of the same protein (UniProt Q12154 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 2WOJ 1.99 Å, ADP-AlF4 complex of S. cerevisiae GET3
- 4XTR 2.05 Å, Structure of Get3 bound to the transmembrane domain of Pep12
- 3ZS9 2.1 Å, S. cerevisiae Get3-ADP-AlF4- complex with a cytosolic Get2 fragment
- 3H84 2.3 Å, Crystal structure of GET3
- 4XVU 2.35 Å, Structure of Get3 bound to the transmembrane domain of Nyv1
- 4XWO 2.75 Å, Structure of Get3 bound to the transmembrane domain of Sec22
- 3A36 2.8 Å, Structural insight into the membrane insertion of tail-anchored proteins by Get3
- 3A37 3.0 Å, Structural insight into the membrane insertion of tail-anchored proteins by Get3
- 3B2E 3.0 Å, Crystal structure of S. cerevisiae Get3 in the open conformation in complex with Get1…
- 3SJA 3.0 Å, Crystal structure of S. cerevisiae Get3 in the open state in complex with Get1 cytosolic…
- 3ZS8 3.0 Å, S. cerevisiae Get3 complexed with a cytosolic Get1 fragment
- 9NS5 3.19 Å, Get3(D57N)-Get4/5 Complex (ATP-bound)
Browse structure collections
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