5CFZ: E. coli FabI in apo form

Crystal structure of E. coli FabI in apo form. Determined by X-ray diffraction at 1.97 Å resolution. Released 9 Dec 2015.

Method: X-ray diffraction
Resolution: 1.97 Å
Organism: Escherichia coli (strain K12)
Chains: 2
Atoms: 3,879
Mol. weight: 65.38 kDa
Released: 9 Dec 2015

Explore 5CFZ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5CFZ contains 35 α-helices and 14 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 17 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	8-11	4	1
α-helix	20-30	11
β-strand	34-39	6	1
α-helix	45-54	10
β-strand	60-62	3	1
α-helix	68-81	14
β-strand	85-90	6	1
α-helix	97-100	4
α-helix	104-107	4
α-helix	110-117	8
α-helix	118-122	5
α-helix	123-131	9
α-helix	132-134	3
β-strand	135-145	11	1
α-helix	147-149	3
α-helix	158-177	20
α-helix	178-180	3
β-strand	182-189	8	1
α-helix	190-191	2
α-helix	203-213	11
α-helix	222-233	12
α-helix	235-237	3
β-strand	244-247	4	1
α-helix	251-253	3

Chain B: 18 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	8-11	4	2
α-helix	20-30	11
β-strand	34-39	6	2
α-helix	42-44	3
α-helix	45-54	10
β-strand	60-62	3	2
α-helix	68-79	12
β-strand	85-90	6	2
α-helix	97-99	3
α-helix	104-107	4
α-helix	110-117	8
α-helix	118-122	5
α-helix	123-131	9
α-helix	132-134	3
β-strand	135-145	11	2
α-helix	147-149	3
α-helix	158-177	20
α-helix	178-180	3
β-strand	182-189	8	2
α-helix	190-191	2
α-helix	205-213	9
α-helix	222-233	12
α-helix	235-237	3
β-strand	244-247	4	2
α-helix	251-253	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI	A, B	protein	305	Escherichia coli (strain K12)	P0AEK4 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>5CFZ_1 Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (chains A, B)
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKMGFLSGKRILVTGVASK
LSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAE
LGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNP
GSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAA
SGIKDFRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMN
ELELK

Primary citation

Crystallographic insights into the structure-activity relationships of diazaborine enoyl-ACP reductase inhibitors. Jordan, C.A., Sandoval, B.A., Serobyan, M.V. et al. Acta Crystallogr F Struct Biol Commun (2015) 71:1521-1530. DOI 10.1107/S2053230X15022098 · PubMed

Other PDB entries of the same protein (UniProt P0AEK4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7UMW 1.54 Å, Crystal structure of E. Coli FabI in complex with NAD and Fabimycin…
7UM8 1.7 Å, Crystal structure of E. Coli FabI in complex with NAD and…
1QSG 1.75 Å, Crystal structure of enoyl reductase inhibition by triclosan
4CV2 1.8 Å, Crystal structure of E. coli FabI in complex with NADH and CG400549
1QG6 1.9 Å, Crystal structure of E. Coli enoyl acyl carrier protein reductase in complex with NAD…
3PJF 1.9 Å, Structure of ENR G93V mutant-NAD+-triclosan complex
4CV3 1.95 Å, Crystal structure of E. coli FabI in complex with NADH and PT166
1C14 2.0 Å, Crystal structure of E coli enoyl reductase-NAD+-triclosan complex
4D46 2.0 Å, Crystal structure of E. coli FabI in complex with NAD and…
5CG1 2.07 Å, Crystal structure of E. coli FabI bound to the carbamoylated benzodiazaborine inhibitor…
1DFI 2.09 Å, X-ray structure of escherichia coli enoyl reductase with bound NAD
5CG2 2.11 Å, Crystal structure of E. coli FabI bound to the thiocarbamoylated benzodiazaborine…

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