5HIT: Calmodulin

Crystal Structure Analysis of Ca2+-calmodulin and a C-terminal EAG1 channel fragment. Determined by X-ray diffraction at 2.85 Å resolution. Released 14 Sept 2016.

Method: X-ray diffraction
Resolution: 2.85 Å
Organisms: Gallus gallus, Mus musculus
Chains: 2
Atoms: 1,257
Mol. weight: 18.7 kDa
Ligands: CA
Released: 14 Sept 2016

Explore 5HIT in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5HIT contains 8 α-helices and 4 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 7 helices, 4 β-strands

Element	Residues	Length	Sheet
α-helix	6-17	12
β-strand	26-27	2	1
α-helix	29-38	10
α-helix	45-54	10
β-strand	63-64	2	1
α-helix	65-92	28
β-strand	100	1	2
α-helix	102-111	10
α-helix	118-128	11
β-strand	136	1	2
α-helix	138-146	9

Chain B: 1 helix, 0 β-strands

Element	Residues	Length	Sheet
α-helix	736-741	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Calmodulin	A	protein	147	Gallus gallus	P62149 (AlphaFold model)
Potassium voltage-gated channel subfamily H member 1	B	protein	17	Mus musculus	Q60603 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5HIT_1 Calmodulin (chains A)
ADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEEFVQMMTA

Sequence of entity 2 (B), FASTA

>5HIT_2 Potassium voltage-gated channel subfamily H member 1 (chains B)
APLILPPDHPVRRLFQR

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	2

Primary citation

Molecular Insights into the Mechanism of Calmodulin Inhibition of the EAG1 Potassium Channel. Marques-Carvalho, M.J., Oppermann, J., Munoz, E. et al. Structure (2016) 24:1742-1754. DOI 10.1016/j.str.2016.07.020 · PubMed

Other PDB entries of the same protein (UniProt P62149 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2O5G 1.08 Å, Calmodulin-smooth muscle light chain kinase peptide complex
3GOF 1.45 Å, Calmodulin bound to peptide from macrophage nitric oxide synthase
3GP2 1.46 Å, Calmodulin bound to peptide from calmodulin kinase II (CaMKII)
2O60 1.55 Å, Calmodulin bound to peptide from neuronal nitric oxide synthase
1AHR 1.8 Å, Calmodulin mutant with a two residue deletion in the central helix
1UP5 1.9 Å, Chicken Calmodulin
2BCX 2.0 Å, Crystal structure of calmodulin in complex with a ryanodine receptor peptide
2VB6 2.3 Å, Myosin VI (MD-insert2-CaM, Delta Insert1) Post-rigor state (crystal form 2)
2BKI 2.9 Å, Myosin VI nucleotide-free (MDinsert2-IQ) crystal structure
6BNV 4.6 Å, CryoEM structure of MyosinVI-actin complex in the rigor (nucleotide-free) state,…
2KZ2 Calmodulin, C-terminal domain, F92E mutant
2M3S Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant

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