5LWV: Human OGT

Human OGT in complex with UDP and fused substrate peptide (HCF1). Determined by X-ray diffraction at 1.9 Å resolution. Released 12 Jul 2017.

Method

X-ray diffraction

Resolution

1.9 Å

Organism

Homo sapiens

Chains

1

Atoms

6,302

Mol. weight

84.47 kDa

Ligands

UDP, PO4

Released

12 Jul 2017

Explore 5LWV in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5LWV contains 44 α-helices and 22 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 44 helices, 22 β-strands

Molecules and chains

>5LWV_1 Host cell factor 1,UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit (chains A)
HHHHHHPPCETHETGTTNTATTATGGGTHADSLNNLANIKREQGNIEEAVRLYRKALEVF
PEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMGNTLKEMQDVQGALQC
YTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKPDFPDAYCNLAHCLQIVC
DWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHGFRKAIAERHGNLCLDKINV
LHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQSIPGMHNPDKFEVFCYALSPDD
GTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIHILVNMNGYTKGARNELFALRPAP
IQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQYSEKLAYMPHTFFIGDHANMFPHLKK
KAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPDVKIVKMKCPDGGDNADSSNTALNMPVI
PMNTIAEAVIEMINRGQIQITINGFSISNGLATTQINNKAATGEEVPRTIIVTTRSQYGL
PEDAIVYCNFNQLYKIDPSTLQMWANILKRVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQ
NRIIFSPVAPKEEHVRRGQLADVCLDTPLCNGHTTGMDVLWAGTPMVTMPGETLASRVAA
SQLTCLGCLELIAKNRQEYEDIAVKLGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELE
RLYLQMWEHYAAGNKPDHMIKPVEVTESA

Ligands and cofactors

Water and common crystallization additives (GOL) are not listed.

Primary citation

Recognition of a glycosylation substrate by the O-GlcNAc transferase TPR repeats. Rafie, K., Raimi, O., Ferenbach, A.T. et al. Open Biol (2017) 7. DOI 10.1098/rsob.170078 · PubMed

Related structures

Other PDB entries of the same protein (UniProt O15294 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 5NPS 1.68 Å, The human O-GlcNAc transferase in complex with a bisubstrate inhibitor
• 4GYW 1.7 Å, Crystal structure of human O-GlcNAc Transferase in complex with UDP and a glycopeptide
• 6EOU 1.75 Å, O-GlcNAc transferase TPR domain with the intellectual disability associated mutation L254F
• 4N39 1.76 Å, Crystal structure of human O-GlcNAc transferase bound to a peptide from HCF-1 pro-repeat…
• 4GYY 1.85 Å, Crystal structure of human O-GlcNAc Transferase with UDP-5SGlcNAc and a peptide substrate
• 5NPR 1.85 Å, The human O-GlcNAc transferase in complex with a thiol-linked bisubstrate inhibitor
• 3TAX 1.88 Å, A Neutral Diphosphate Mimic Crosslinks the Active Site of Human O-GlcNAc Transferase
• 4N3A 1.88 Å, Crystal Structure of human O-GlcNAc transferase bound to a peptide from HCF-1 pro-repeat…
• 4GZ3 1.9 Å, Crystal structure of human O-GlcNAc Transferase with UDP and a thioglycopeptide
• 6TKA 1.91 Å, Crystal structure of human O-GlcNAc transferase bound to substrate 7 and a peptide from…
• 3PE4 1.95 Å, Structure of human O-GlcNAc transferase and its complex with a peptide substrate
• 6MA3 2.0 Å, Crystal structure of human O-GlcNAc transferase bound to a peptide from HCF-1 pro-repeat…

Browse structure collections

About this viewer

MolViewer shows 5LWV directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.