5UN7: Human POT1-TPP1 telomeric complex

Structure of the human POT1-TPP1 telomeric complex. Determined by X-ray diffraction at 2.1 Å resolution. Released 12 Apr 2017.

Method: X-ray diffraction
Resolution: 2.1 Å
Organism: Homo sapiens
Chains: 2
Atoms: 3,374
Mol. weight: 44.16 kDa
Ligands: ZN
Released: 12 Apr 2017

Explore 5UN7 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5UN7 contains 21 α-helices and 26 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 25 β-strands

Element	Residues	Length	Sheet
β-strand	335-337	3	1
α-helix	344	1
β-strand	345	1	1
α-helix	348-352	5
α-helix	355	1
β-strand	359-370	12	1
α-helix	374-376	3
β-strand	378-381	4	1
β-strand	388-390	3	1
α-helix	391-393	3
α-helix	394-403	10
β-strand	406	1	2
α-helix	413-415	3
β-strand	419-424	6	3
β-strand	427	1	4
β-strand	433	1	2
β-strand	434-439	6	3
β-strand	441	1	5
β-strand	444	1	5
α-helix	448-450	3
β-strand	452-456	5	3
α-helix	460-469	10
β-strand	472-475	4	3
β-strand	476-478	3	6
β-strand	483-485	3	6
β-strand	493-495	3	3
β-strand	498-500	3	3
β-strand	502	1	7
α-helix	512-517	6
α-helix	526-532	7
β-strand	537	1	7
β-strand	539-549	11	1
β-strand	554-561	8	1
α-helix	570-573	4
α-helix	577-590	14
α-helix	597-599	3
α-helix	601-602	2
β-strand	603-613	11	1
β-strand	618-625	8	1
β-strand	627-629	3	1

Chain B: 6 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	267-278	12
β-strand	282	1	4
α-helix	287-289	3
α-helix	292-299	8
α-helix	304-306	3
α-helix	310-313	4
α-helix	317-324	8

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Protection of telomeres protein 1	A	protein	308	Homo sapiens	Q9NUX5 (AlphaFold model)
Adrenocortical dysplasia protein homolog	B	protein	86	Homo sapiens	Q96AP0 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>5UN7_1 Protection of telomeres protein 1 (chains A)
SNAQQLSATILTDHQYLERTPLCAILKQKAPQQYRIRAKLRSYKPRRLFQSVKLHCPKCH
LLQEVPHEGDLDIIFQDGATKTPDVKLQNTSLYDSKIWTTKNQKGRKVAVHFVKNNGILP
LSNECLLLIEGGTLSEICKLSNKFNSVIPVRSGHEDLELLDLSAPFLIQGTIHHYGCKQC
SSLRSIQNLNSLVDKTSWIPSSVAEALGIVPLQYVFVMTFTLDDGTGVLEAYLMDSDKFF
QIPASEVLMDDDLQKSVDMIMDMFCPPGIKIDAYPWLECFIKSYNVTNGTDNQICYQIFD
TTVAEDVI

Sequence of entity 2 (B), FASTA

>5UN7_2 Adrenocortical dysplasia protein homolog (chains B)
SNASQLLDEMREDQEHQGALVCLAESCLTLEGPCTAPPVTHWAASRCKATGEAVYTVPSS
MLCISENDQLILSSLGPCQRTQGPEL

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	1

Water and common crystallization additives (K) are not listed.

Primary citation

Structural and functional analysis of the human POT1-TPP1 telomeric complex. Rice, C., Shastrula, P.K., Kossenkov, A.V. et al. Nat Commun (2017) 8:14928-14928. DOI 10.1038/ncomms14928 · PubMed

Other PDB entries of the same protein (UniProt Q9NUX5 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1XJV 1.73 Å, Crystal structure of human POT1 bound to telomeric single-stranded DNA (TTAGGGTTAG)
3KJO 1.8 Å, Crystal Structure of hPOT1V2-dTrUd(AGGGTTAG)
3KJP 1.8 Å, Crystal Structure of hPOT1V2-GGTTAGGGTTAG
5H65 2.1 Å, Crystal structure of human POT1 and TPP1
8SH0 2.16 Å, Structure of human POT1 DNA binding domain bound to a 5'-phosphorylated junction of a…
7S1O 2.55 Å, Structure of human POT1C
7S1U 2.55 Å, Structure of human POT1C
8SH1 2.6 Å, Structure of human POT1 DNA binding domain bound to a 5'-phosphorylated junction of a…
7S1T 2.9 Å, Structure of the human POT1-TPP1 complex
8SOJ 3.8 Å, Cryo-EM structure of human CST bound to POT1(ESDL)/TPP1 in the absence of telomeric ssDNA
7QXB 3.9 Å, Cryo-EM map of human telomerase-DNA-TPP1-POT1 complex (sharpened map)
7QXS 3.9 Å, Cryo-EM structure of human telomerase-DNA-TPP1-POT1 complex (with POT1 side chains)

Browse structure collections

About this viewer

MolViewer shows 5UN7 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.