5Z57: Human activated spliceosome

Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom. Determined by electron microscopy at 6.5 Å resolution. Released 19 Sept 2018.

Method: Electron microscopy
Resolution: 6.5 Å
Organisms: Homo sapiens, unidentified adenovirus
Chains: 57
Atoms: 113,433
Mol. weight: 3510.09 kDa
Ligands: IHP, GTP, MG, ZN
Released: 19 Sept 2018

Explore 5Z57 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

5Z57 contains 758 α-helices and 646 β-strands across 53 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 1: 74 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	85-88	4
α-helix	104-107	4
α-helix	120-125	6
α-helix	174-176	3
α-helix	178-189	12
α-helix	210-215	6
α-helix	218-220	3
α-helix	227-234	8
α-helix	238-254	17
α-helix	312-325	14
α-helix	327-334	8
α-helix	401-407	7
β-strand	413-414	2	24
α-helix	421-433	13
α-helix	472-479	8
α-helix	491-497	7
α-helix	500-505	6
α-helix	509-521	13
α-helix	523-526	4
α-helix	528-539	12
α-helix	547-560	14
α-helix	565-570	6
α-helix	571-582	12
α-helix	587-603	17
α-helix	606-613	8
α-helix	614-616	3
α-helix	622-639	18
α-helix	641-651	11
α-helix	658-675	18
α-helix	676-682	7
α-helix	683-690	8
α-helix	691-693	3
α-helix	700-715	16
α-helix	721-727	7
α-helix	728-737	10
α-helix	741-753	13
α-helix	754-756	3
α-helix	761-775	15
α-helix	776-778	3
α-helix	782-796	15
α-helix	803-809	7
α-helix	811-813	3
α-helix	814-818	5
α-helix	828-844	17
α-helix	846-853	8
α-helix	854-858	5
α-helix	862-878	17
α-helix	881-883	3
α-helix	887-901	15
α-helix	908-921	14
α-helix	922-928	7
α-helix	929-941	13
α-helix	944-964	21
α-helix	968-981	14
α-helix	987-1001	15
α-helix	1013-1020	8
α-helix	1021-1025	5
α-helix	1029-1044	16
α-helix	1052-1062	11
α-helix	1063-1067	5
α-helix	1071-1088	18
α-helix	1090-1098	9
α-helix	1110-1122	13
α-helix	1125-1135	11
α-helix	1141-1158	18
α-helix	1159-1161	3
α-helix	1162-1177	16
α-helix	1182-1198	17
α-helix	1205-1215	11
α-helix	1216-1219	4
α-helix	1224-1241	18
α-helix	1243-1250	8
α-helix	1259-1274	16
α-helix	1281-1283	3
α-helix	1298-1301	4

Chain 2: 14 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	464-467	4
α-helix	472-474	3
α-helix	478-481	4
α-helix	485-492	8
α-helix	499-501	3
α-helix	515-517	3
α-helix	526-529	4
α-helix	533-536	4
α-helix	549-557	9
α-helix	570-573	4
α-helix	574-578	5
β-strand	605	1	37
β-strand	608	1	37
α-helix	611-617	7
α-helix	631-633	3
α-helix	635-638	4
β-strand	651	1	38
β-strand	655	1	38
β-strand	683	1	39
β-strand	686	1	39

Chain 3: 16 helices, 102 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	61
β-strand	14-20	7	62
β-strand	29-34	6	62
β-strand	37-42	6	62
β-strand	51-57	7	62
β-strand	62-69	8	63
β-strand	76-82	7	63
β-strand	86-93	8	63
β-strand	98-106	9	63
β-strand	120-123	4	64
β-strand	129-133	5	64
β-strand	136	1	65
β-strand	139-146	8	64
β-strand	152-157	6	64
β-strand	165-172	8	65
β-strand	181-188	8	65
α-helix	190-193	4
α-helix	205-206	2
β-strand	207-214	8	65
β-strand	219-227	9	65
β-strand	232-238	7	66
β-strand	247-252	6	66
β-strand	255-259	5	66
α-helix	264-266	3
β-strand	267-270	4	66
β-strand	293-294	2	67
β-strand	299-300	2	67
β-strand	301-303	3	68
β-strand	309-318	10	68
β-strand	321-332	12	68
β-strand	336-338	3	69
β-strand	341-342	2	70
β-strand	346-349	4	70
β-strand	350-351	2	69
β-strand	356-360	5	70
β-strand	372-374	3	68
β-strand	396-401	6	70
β-strand	407-413	7	71
α-helix	421	1
β-strand	422-427	6	71
α-helix	430-432	3
β-strand	434-438	5	71
β-strand	442	1	72
β-strand	444-450	7	73
β-strand	458-462	5	74
β-strand	473-477	5	74
β-strand	482-486	5	74
β-strand	492-493	2	74
β-strand	507-510	4	75
β-strand	516-520	5	75
β-strand	523-527	5	75
β-strand	533-537	5	75
α-helix	538	1
β-strand	544-550	7	76
β-strand	553-558	6	76
β-strand	562	1	77
β-strand	563-568	6	76
β-strand	574-576	3	76
β-strand	582	1	77
β-strand	592	1	78
α-helix	593-596	4
β-strand	605-608	4	78
β-strand	613-618	6	78
β-strand	626-632	7	78
β-strand	637-644	8	79
β-strand	663-669	7	79
β-strand	674-676	3	79
β-strand	687-689	3	79
β-strand	700-706	7	80
β-strand	709-715	7	80
β-strand	719-724	6	80
β-strand	729-733	5	80
β-strand	734	1	72
β-strand	741-745	5	73
β-strand	753-758	6	73
β-strand	761-767	7	73
β-strand	777-780	4	71
β-strand	784-791	8	81
β-strand	796-804	9	81
α-helix	809-824	16
α-helix	839-844	6
β-strand	862-869	8	81
β-strand	876-881	6	81
β-strand	887-894	8	82
β-strand	904-911	8	82
β-strand	914	1	83
β-strand	919	1	83
β-strand	922-928	7	82
β-strand	931	1	84
β-strand	936	1	84
β-strand	939-945	7	82
β-strand	949-955	7	85
β-strand	958-963	6	85
β-strand	966-971	6	85
β-strand	978-983	6	85
β-strand	990-995	6	86
β-strand	1000-1004	5	86
β-strand	1007	1	87
β-strand	1009-1014	6	86
β-strand	1021-1026	6	86
β-strand	1031	1	87
β-strand	1033-1037	5	88
β-strand	1043-1048	6	88
β-strand	1052-1057	6	88
β-strand	1088	1	86
β-strand	1091-1096	6	88
β-strand	1104-1107	4	61
α-helix	1113-1115	3
β-strand	1116-1120	5	61
β-strand	1126-1131	6	61
α-helix	1135-1151	17
α-helix	1160-1164	5
β-strand	1171	1	88
β-strand	1173-1175	3	61
α-helix	1176-1179	4
α-helix	1180-1182	3
α-helix	1187-1196	10
α-helix	1201-1215	15

Chain 4: 3 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	15-17	3	102
α-helix	26-34	9
β-strand	41-43	3	102
α-helix	45-46	2
β-strand	47	1	103
β-strand	52	1	103
β-strand	57-60	4	102
α-helix	64-74	11
β-strand	78-79	2	104
β-strand	82-83	2	104
β-strand	87-88	2	102

Chain 5: 3 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	20-24	5	24
α-helix	32-39	8
β-strand	45-50	6	24
β-strand	60-64	5	24
α-helix	67-77	11
β-strand	81-82	2	112
β-strand	85-86	2	112
β-strand	88-91	4	24
α-helix	106-115	10

Chain 6: 5 helices, 10 β-strands

Element	Residues	Length	Sheet
β-strand	8-10	3	113
α-helix	13-15	3
β-strand	18-19	2	114
α-helix	20	1
β-strand	22	1	115
β-strand	42	1	116
β-strand	44-45	2	114
α-helix	52-54	3
β-strand	57	1	117
β-strand	64	1	117
β-strand	67	1	115
β-strand	70	1	116
α-helix	74-77	4
α-helix	80-82	3
β-strand	88-90	3	113

Chain 7: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	28-45	18
α-helix	47-56	10
α-helix	61-71	11

Chains a and h: 2 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	5-12	8
β-strand	17-22	6	111
β-strand	27-35	9	111
β-strand	41-49	9	111
β-strand	55-63	9	111
α-helix	65-67	3
β-strand	68-73	6	111

41 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Pre-mRNA-processing-splicing factor 8	A	protein	2335	Homo sapiens	Q6P2Q9 (AlphaFold model)
U5 snRNA	B	RNA	117	Homo sapiens
116 kDa U5 small nuclear ribonucleoprotein component	C	protein	972	Homo sapiens	Q15029 (AlphaFold model)
U5 small nuclear ribonucleoprotein 200 kDa helicase	D	protein	2136	Homo sapiens	O75643 (AlphaFold model)
U5 small nuclear ribonucleoprotein 40 kDa protein	E	protein	357	Homo sapiens	Q96DI7 (AlphaFold model)
Small nuclear ribonucleoprotein Sm D3	a, h	protein	126	Homo sapiens	P62318
Small nuclear ribonucleoprotein-associated proteins B and B'	b, i	protein	231	Homo sapiens	P14678
Small nuclear ribonucleoprotein Sm D1	c, j	protein	119	Homo sapiens	P62314
Small nuclear ribonucleoprotein Sm D2	d, k	protein	118	Homo sapiens	P62316
Small nuclear ribonucleoprotein F	f, m	protein	86	Homo sapiens	P62306
Small nuclear ribonucleoprotein E	e, l	protein	92	Homo sapiens	P62304
Small nuclear ribonucleoprotein G	g, n	protein	76	Homo sapiens	P62308

35 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>5Z57_1 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Sequence of entity 2 (B), FASTA

>5Z57_2 U5 snRNA (chains B)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGGCCUUGCUUUGGCAAGGCUA

Sequence of entity 3 (C), FASTA

>5Z57_3 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM

Sequence of entity 4 (D), FASTA

>5Z57_4 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains D)
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTK
PQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFI
QAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKIT
DYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTL
SANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDR
ECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFL
YQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS
HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKT
LNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIY
IAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKG
GERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYED
VATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQ
VLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPY
GFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR
WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDM
LNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTA
ALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSL
SSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMAD
MVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVV
KKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKV
ELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE
PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESL
YQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYIT
PMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWK
QRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDV
AHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVI
VFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYL
HEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP
IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEI
VTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSK
CISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPI
RHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAI
RLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVE
SVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQ
LEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG
AHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Sequence of entity 5 (E), FASTA

>5Z57_5 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ

Sequence of entity 6 (a, h), FASTA

>5Z57_6 Small nuclear ribonucleoprotein Sm D3 (chains a, h)
MSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQ
VYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRGN
IFQKRR

Sequence of entity 7 (b, i), FASTA

>5Z57_7 Small nuclear ribonucleoprotein-associated proteins B and B' (chains b, i)
MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAE
REEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPM
PQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMG
RGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGLL

Sequence of entity 8 (c, j), FASTA

>5Z57_8 Small nuclear ribonucleoprotein Sm D1 (chains c, j)
MKLVRFLMKLSHETVTIELKNGTQVHGTITGVDVSMNTHLKAVKMTLKNREPVQLETLSI
RGNNIRYFILPDSLPLDTLLVDVEPKVKSKKREAVAGRGRGRGRGRGRGRGRGRGGPRR

Sequence of entity 9 (d, k), FASTA

>5Z57_9 Small nuclear ribonucleoprotein Sm D2 (chains d, k)
MSLLNKPKSEMTPEELQKREEEEFNTGPLSVLTQSVKNNTQVLINCRNNKKLLGRVKAFD
RHCNMVLENVKEMWTEVPKSGKGKKKSKPVNKDRYISKMFLRGDSVIVVLRNPLIAGK

Sequence of entity 10 (f, m), FASTA

>5Z57_10 Small nuclear ribonucleoprotein F (chains f, m)
MSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLG
EVLIRCNNVLYIRGVEEEEEDGEMRE

Sequence of entity 11 (e, l), FASTA

>5Z57_11 Small nuclear ribonucleoprotein E (chains e, l)
MAYRGQGQKVQKVMVQPINLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDD
AEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Sequence of entity 12 (g, n), FASTA

>5Z57_12 Small nuclear ribonucleoprotein G (chains g, n)
MSKAHPPELKKFMDKKLSLKLNGGRHVQGILRGFDPFMNLVIDECVEMATSGQQNNIGMV
VIRGNSIIMLEALERV

Ligands and cofactors

ID	Name	Formula	Copies
IHP	Inositol hexakisphosphate	C6 H18 O24 P6	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
MG	Magnesium ion	Mg	6
ZN	Zinc ion	Zn	10
ALA	Alanine	C3 H7 N O2	1

Primary citation

Structure of the human activated spliceosome in three conformational states. Zhang, X., Yan, C., Zhan, X. et al. Cell Res (2018) 28:307-322. DOI 10.1038/cr.2018.14 · PubMed

Other PDB entries of the same protein (UniProt Q6P2Q9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4JK8 1.15 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
4JKB 1.3 Å, Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
4JKA 1.32 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
4JK7 1.4 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
4JK9 1.5 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
4JKC 1.5 Å, Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
4JKD 1.55 Å, Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
4JKE 1.65 Å, Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
4JKG 1.8 Å, Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain…
4JKH 1.8 Å, Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
3ENB 1.85 Å, Crystal Structure of PRP8 core domain IV
3LRU 1.85 Å, hPRP8 Non-Native Subdomain

Browse structure collections

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