Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate. Determined by electron microscopy at 4.7 Å resolution. Released 9 May 2018.
Explore 5ZAL in 3D Show helices and sheets RCSB PDB PDBe
5ZAL contains 63 α-helices and 38 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 46-53 | 8 | |
| α-helix | 54-56 | 3 | |
| β-strand | 59 | 1 | 1 |
| α-helix | 70-83 | 14 | |
| β-strand | 97-99 | 3 | 2 |
| α-helix | 103-113 | 11 | |
| β-strand | 121-123 | 3 | 2 |
| α-helix | 141-144 | 4 | |
| β-strand | 147-150 | 4 | 2 |
| α-helix | 152-159 | 8 | |
| β-strand | 171-173 | 3 | 2 |
| α-helix | 177-179 | 3 | |
| α-helix | 186-191 | 6 | |
| β-strand | 201-203 | 3 | 2 |
| α-helix | 219-230 | 12 | |
| β-strand | 232 | 1 | 1 |
| β-strand | 253-254 | 2 | 3 |
| β-strand | 260 | 1 | 4 |
| α-helix | 263-265 | 3 | |
| α-helix | 269-284 | 16 | |
| α-helix | 297-312 | 16 | |
| α-helix | 317-335 | 19 | |
| α-helix | 343-357 | 15 | |
| α-helix | 359-363 | 5 | |
| β-strand | 371 | 1 | 5 |
| α-helix | 379-382 | 4 | |
| β-strand | 388 | 1 | 4 |
| β-strand | 445 | 1 | 6 |
| β-strand | 446 | 1 | 3 |
| α-helix | 454-460 | 7 | |
| β-strand | 465 | 1 | 5 |
| α-helix | 499-509 | 11 | |
| α-helix | 512-515 | 4 | |
| β-strand | 518 | 1 | 6 |
| β-strand | 534-537 | 4 | 3 |
| α-helix | 543-550 | 8 | |
| β-strand | 560-563 | 4 | 3 |
| α-helix | 570-572 | 3 | |
| α-helix | 575-591 | 17 | |
| α-helix | 635-638 | 4 | |
| α-helix | 691-697 | 7 | |
| α-helix | 700-705 | 6 | |
| α-helix | 715-723 | 9 | |
| β-strand | 770-777 | 8 | 7 |
| α-helix | 783-785 | 3 | |
| α-helix | 795-797 | 3 | |
| β-strand | 800-805 | 6 | 7 |
| β-strand | 816-819 | 4 | 7 |
| β-strand | 824-831 | 8 | 7 |
| α-helix | 840-856 | 17 | |
| β-strand | 878-884 | 7 | 7 |
| β-strand | 889-892 | 4 | 7 |
| α-helix | 894-902 | 9 | |
| β-strand | 931-934 | 4 | 8 |
| β-strand | 945-951 | 7 | 8 |
| α-helix | 968-974 | 7 | |
| α-helix | 985-986 | 2 | |
| β-strand | 987-992 | 6 | 8 |
| α-helix | 1003-1010 | 8 | |
| α-helix | 1016-1018 | 3 | |
| α-helix | 1023-1030 | 8 | |
| β-strand | 1032-1033 | 2 | 8 |
| β-strand | 1038-1041 | 4 | 8 |
| α-helix | 1045-1051 | 7 | |
| α-helix | 1054-1071 | 18 | |
| α-helix | 1290-1292 | 3 | |
| α-helix | 1295-1300 | 6 | |
| α-helix | 1313-1333 | 21 | |
| α-helix | 1340-1350 | 11 | |
| α-helix | 1353-1361 | 9 | |
| α-helix | 1365-1368 | 4 | |
| α-helix | 1559-1572 | 14 | |
| α-helix | 1576-1583 | 8 | |
| α-helix | 1593-1596 | 4 | |
| α-helix | 1618-1620 | 3 | |
| α-helix | 1656-1662 | 7 | |
| α-helix | 1667-1672 | 6 | |
| α-helix | 1680-1686 | 7 | |
| β-strand | 1688 | 1 | 9 |
| α-helix | 1694-1696 | 3 | |
| α-helix | 1702-1721 | 20 | |
| α-helix | 1731-1738 | 8 | |
| α-helix | 1742-1751 | 10 | |
| β-strand | 1759 | 1 | 9 |
| α-helix | 1763-1775 | 13 | |
| α-helix | 1808-1822 | 15 | |
| α-helix | 1829-1847 | 19 | |
| α-helix | 1853-1860 | 8 | |
| β-strand | 1865-1866 | 2 | 10 |
| β-strand | 1882-1884 | 3 | 10 |
| β-strand | 1888-1890 | 3 | 10 |
| α-helix | 1897-1910 | 14 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| α-helix | 294-301 | 8 | |
| β-strand | 309-310 | 2 | 11 |
| β-strand | 313-317 | 5 | 12 |
| α-helix | 318 | 1 | |
| β-strand | 323-325 | 3 | 12 |
| β-strand | 328-329 | 2 | 11 |
| β-strand | 339 | 1 | 12 |
| α-helix | 344-352 | 9 | |
| α-helix | 353-355 | 3 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Endoribonuclease Dicer | A | protein | 1922 | Homo sapiens | Q9UPY3 (AlphaFold model) |
| RISC-loading complex subunit TARBP2 | B | protein | 366 | Homo sapiens | Q15633 (AlphaFold model) |
| RNA (73-mer) | C | RNA | 73 | Homo sapiens |
>5ZAL_1 Endoribonuclease Dicer (chains A) MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNT IVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLK VGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLA ILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDL VVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISK QILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCE EHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDE DEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFIT GHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLP TEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETD IDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPD GTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETV KYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTP LPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSL QMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDI EKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKF PSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKR KAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGV GVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRT SSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGN QLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAV MPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGD SFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNW LPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYED DFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFED FDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIA DCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVS CAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNK AYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALV NNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEE DIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEM EPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVP NS
>5ZAL_2 RISC-loading complex subunit TARBP2 (chains B) MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQP NFTFRVTVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPE DIPVFTAAAAATPVPSVVLTRSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTV TQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEP DDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSE LSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGEAARRALQYLK IMAGSK
>5ZAL_3 RNA (73-mer) (chains C) UGAGGUAGUAGGUUGUAUAGUUUUAGGGUCACACCCACCACUGGGAGAUAACUAUACAAU CUACUGUCUUACC
Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Liu, Z., Wang, J., Cheng, H. et al. Cell (2018) 173:1191-1203.e12. DOI 10.1016/j.cell.2018.03.080 · PubMed
Other PDB entries of the same protein (UniProt Q9UPY3 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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