6I4G: Actin-1

Crystal Structure of Plasmodium falciparum actin I (H74Q) in the Mg-K-ATP state. Determined by X-ray diffraction at 2.0 Å resolution. Released 26 Jun 2019.

Method: X-ray diffraction
Resolution: 2.0 Å
Organisms: Plasmodium falciparum, Mus musculus
Chains: 4
Atoms: 8,066
Mol. weight: 114.09 kDa
Ligands: SCN, CA, MG, ATP
Released: 26 Jun 2019

Explore 6I4G in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6I4G contains 60 α-helices and 56 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 26 helices, 22 β-strands

Element	Residues	Length	Sheet
α-helix	7-8	2
β-strand	9-13	5	1
β-strand	17-22	6	1
β-strand	30-33	4	1
β-strand	36-38	3	2
β-strand	54-55	2	2
α-helix	57-61	5
α-helix	66	1
β-strand	67-69	3	2
β-strand	72-73	2	3
β-strand	76-77	2	3
α-helix	80-88	9
α-helix	89-95	7
β-strand	97	1	4
α-helix	99-101	3
β-strand	104-108	5	1
α-helix	114-126	13
β-strand	132-137	6	1
α-helix	138-145	8
β-strand	151-156	6	5
β-strand	161-167	7	5
β-strand	170-171	2	5
α-helix	173-175	3
β-strand	177-179	3	5
α-helix	183-197	15
α-helix	204-217	14
β-strand	219	1	6
α-helix	224-232	9
β-strand	239-242	4	7
β-strand	248-251	4	7
α-helix	254-260	7
α-helix	265-268	4
α-helix	272-274	3
α-helix	275-284	10
α-helix	288-290	3
α-helix	291-295	5
β-strand	298-301	4	5
α-helix	303-305	3
β-strand	308	1	6
α-helix	310-321	12
α-helix	327-328	2
β-strand	330-331	2	5
α-helix	336-338	3
α-helix	339-347	9
α-helix	351-355	5
β-strand	358-359	2	1
α-helix	360-366	7
α-helix	368-371	4

Chain B: 21 helices, 20 β-strands

Element	Residues	Length	Sheet
β-strand	9-13	5	8
β-strand	17-22	6	8
β-strand	30-33	4	8
β-strand	36-38	3	9
β-strand	54-55	2	9
α-helix	57-61	5
β-strand	67-69	3	9
β-strand	72-73	2	10
β-strand	76-77	2	10
α-helix	80-88	9
α-helix	89-95	7
α-helix	99-101	3
β-strand	104-108	5	8
α-helix	114-126	13
β-strand	132-137	6	8
α-helix	138-145	8
β-strand	151-156	6	11
β-strand	161-167	7	11
β-strand	170-171	2	11
α-helix	173-175	3
β-strand	177-179	3	11
α-helix	183-197	15
α-helix	204-217	14
α-helix	224-232	9
β-strand	239-242	4	12
β-strand	248-251	4	12
α-helix	254-260	7
α-helix	265-268	4
α-helix	275-284	10
β-strand	286	1	4
α-helix	288-295	8
β-strand	298-301	4	11
α-helix	303-305	3
α-helix	310-321	12
β-strand	330-331	2	11
α-helix	336-338	3
α-helix	339-347	9
α-helix	351-355	5
β-strand	358-359	2	8
α-helix	360-366	7
α-helix	368-372	5

Chain G: 6 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	8-10	3
β-strand	16-23	8	13
β-strand	26-29	4	13
α-helix	30-31	2
α-helix	32-34	3
β-strand	37-39	3	14
β-strand	43-50	8	13
β-strand	58-65	8	13
α-helix	71-87	17
β-strand	93-98	6	13
α-helix	104-108	5
β-strand	115-117	3	14
α-helix	121-123	3

Chain H: 7 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	6-10	5
β-strand	16-22	7	15
β-strand	27-29	3	15
α-helix	30-31	2
α-helix	32-34	3
β-strand	37-39	3	16
β-strand	43-51	9	15
β-strand	57-65	9	15
α-helix	71-87	17
α-helix	92	1
β-strand	93-98	6	15
α-helix	104-107	4
β-strand	115-117	3	16
α-helix	121-123	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin-1	A, B	protein	378	Plasmodium falciparum	Q8I4X0 (AlphaFold model)
Gelsolin	G, H	protein	127	Mus musculus	P13020 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>6I4G_1 Actin-1 (chains A, B)
GAMGEEDVQALVVDNGSGNVKAGVAGDDAPRSVFPSIVGRPKNPGIMVGMEEKDAFVGDE
AQTKRGILTLKYPIEQGIVTNWDDMEKIWHHTFYNELRAAPEEHPVLLTEAPLNPKGNRE
RMTQIMFESFNVPAMYVAIQAVLSLYSSGRTTGIVLDSGDGVSHTVPIYEGYALPHAIMR
LDLAGRDLTEYLMKILHERGYGFSTSAEKEIVRDIKEKLCYIALNFDEEMKTSEQSSDIE
KSYELPDGNIITVGNERFRCPEALFQPSFLGKEAAGIHTTTFNSIKKCDVDIRKDLYGNI
VLSGGTTMYEGIGERLTRDITTLAPSTMKIKVVAPPERKYSVWIGGSILSSLSTFQQMWI
TKEEYDESGPSIVHRKCF

Sequence of entity 2 (G, H), FASTA

>6I4G_2 Gelsolin (chains G, H)
GPMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGNLQ
YDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESSTFSGYFKSGLKYKK
GGVASGF

Ligands and cofactors

ID	Name	Formula	Copies
SCN	Thiocyanate ion	C N S	1
CA	Calcium ion	Ca	4
MG	Magnesium ion	Mg	2
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	2

Water and common crystallization additives (BME, K) are not listed.

Primary citation

Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation. Kumpula, E.P., Lopez, A.J., Tajedin, L. et al. PLoS Biol (2019) 17:e3000315-e3000315. DOI 10.1371/journal.pbio.3000315 · PubMed

Other PDB entries of the same protein (UniProt Q8I4X0 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6I4E 1.22 Å, Crystal Structure of Plasmodium falciparum actin I in the Mg-ADP state
6I4D 1.24 Å, Crystal Structure of Plasmodium falciparum actin I in the Mg-K-ATP/ADP state
4CBU 1.3 Å, Crystal structure of Plasmodium falciparum actin I
5MVV 1.4 Å, Crystal structure of Plasmodium falciparum actin I- gelsolin segment 1 -CdATP complex
6I4H 1.4 Å, Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Ca-ATP state
6I4F 1.5 Å, Crystal Structure of Plasmodium falciparum actin I (A272W mutant) in the Mg-K-ATP/ADP…
6I4J 1.5 Å, Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-ADP state
6I4K 1.83 Å, Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Ca-ATP state
6I4L 1.83 Å, Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Mg-K-ATP/ADP…
6I4I 1.9 Å, Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-K-ADP-AlFn…
6TU4 2.6 Å, Structure of Plasmodium Actin1 filament
6TU7 3.1 Å, Structure of PfMyoA decorated Plasmodium Act1 filament

Browse structure collections

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