6I4L: Actin-1

Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Mg-K-ATP/ADP state. Determined by X-ray diffraction at 1.83 Å resolution. Released 26 Jun 2019.

Method: X-ray diffraction
Resolution: 1.83 Å
Organisms: Plasmodium falciparum, Mus musculus
Chains: 2
Atoms: 4,276
Mol. weight: 57.43 kDa
Ligands: CA, SCN, MG, ADP
Released: 26 Jun 2019

Explore 6I4L in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6I4L contains 29 α-helices and 26 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 23 helices, 19 β-strands

Element	Residues	Length	Sheet
α-helix	7-8	2
β-strand	9-13	5	1
β-strand	17-22	6	1
β-strand	30-33	4	1
β-strand	36-39	4	2
β-strand	54-55	2	2
α-helix	57-61	5
β-strand	66-69	4	2
β-strand	72-73	2	3
β-strand	76-77	2	3
α-helix	80-88	9
α-helix	89-95	7
α-helix	99-101	3
β-strand	104-108	5	1
α-helix	114-126	13
β-strand	132-137	6	1
α-helix	138-145	8
β-strand	151-156	6	4
β-strand	161-167	7	4
β-strand	170-171	2	4
α-helix	173-175	3
β-strand	177-179	3	4
α-helix	183-193	11
α-helix	194-197	4
α-helix	204-217	14
α-helix	224-232	9
β-strand	239-242	4	5
β-strand	248-251	4	5
α-helix	254-260	7
α-helix	265-268	4
α-helix	275-284	10
α-helix	288-290	3
α-helix	291-295	5
β-strand	298-301	4	4
α-helix	303-305	3
α-helix	310-321	12
β-strand	330-331	2	4
α-helix	336-338	3
α-helix	339-347	9
β-strand	358-359	2	1
α-helix	360-366	7
α-helix	368-371	4

Chain G: 6 helices, 7 β-strands

Element	Residues	Length	Sheet
α-helix	6-10	5
β-strand	16-23	8	6
β-strand	26-29	4	6
α-helix	30-31	2
α-helix	32-34	3
β-strand	37-39	3	7
β-strand	43-50	8	6
β-strand	58-65	8	6
α-helix	71-87	17
β-strand	93-98	6	6
α-helix	104-107	4
β-strand	115-117	3	7
α-helix	121-123	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Actin-1	A	protein	378	Plasmodium falciparum	Q8I4X0 (AlphaFold model)
Gelsolin	G	protein	127	Mus musculus	P13020 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6I4L_1 Actin-1 (chains A)
GAMGEEDVQALVVDNGSGNVKAGVAGDDAPRSVFPSIVGRPKNPGIMVGMEEKDAFVGDE
AQTKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRAAPEEHPVLLTEAPLNPKANRE
RMTQIMFESFNVPAMYVAIQAVLSLYSSGRTTGIVLDSGDGVSHTVPIYEGYALPHAIMR
LDLAGRDLTEYLMKILHERGYGFSTSAEKEIVRDIKEKLCYIALNFDEEMKTSEQSSDIE
KSYELPDGNIITVGNERFRCPEALFQPSFLGKEAAGIHTTTFNSIKKCDVDIRKDLYGNI
VLSGGTTMYEGIGERLTRDITTLAPSTMKIKVVAPPERKYSVWIGGSILSSLSTFQQMWI
TKEEYDESGPSIVHRKCF

Sequence of entity 2 (G), FASTA

>6I4L_2 Gelsolin (chains G)
GPMVVEHPEFLKAGKEPGLQIWRVEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGNLQ
YDLHYWLGNECSQDESGAAAIFTVQLDDYLNGRAVQHREVQGFESSTFSGYFKSGLKYKK
GGVASGF

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	2
SCN	Thiocyanate ion	C N S	2
MG	Magnesium ion	Mg	1
ADP	Adenosine-5'-diphosphate	C10 H15 N5 O10 P2	1
ATP	Adenosine-5'-triphosphate	C10 H16 N5 O13 P3	1

Primary citation

Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation. Kumpula, E.P., Lopez, A.J., Tajedin, L. et al. PLoS Biol (2019) 17:e3000315-e3000315. DOI 10.1371/journal.pbio.3000315 · PubMed

Other PDB entries of the same protein (UniProt Q8I4X0 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6I4E 1.22 Å, Crystal Structure of Plasmodium falciparum actin I in the Mg-ADP state
6I4D 1.24 Å, Crystal Structure of Plasmodium falciparum actin I in the Mg-K-ATP/ADP state
4CBU 1.3 Å, Crystal structure of Plasmodium falciparum actin I
5MVV 1.4 Å, Crystal structure of Plasmodium falciparum actin I- gelsolin segment 1 -CdATP complex
6I4H 1.4 Å, Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Ca-ATP state
6I4F 1.5 Å, Crystal Structure of Plasmodium falciparum actin I (A272W mutant) in the Mg-K-ATP/ADP…
6I4J 1.5 Å, Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-ADP state
6I4K 1.83 Å, Crystal Structure of Plasmodium falciparum actin I (G115A mutant) in the Ca-ATP state
6I4I 1.9 Å, Crystal Structure of Plasmodium falciparum actin I (F54Y mutant) in the Mg-K-ADP-AlFn…
6I4G 2.0 Å, Crystal Structure of Plasmodium falciparum actin I (H74Q) in the Mg-K-ATP state
6TU4 2.6 Å, Structure of Plasmodium Actin1 filament
6TU7 3.1 Å, Structure of PfMyoA decorated Plasmodium Act1 filament

Browse structure collections

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