6P5B: MavC

Crystal Structure of MavC in Complex with Ub-UbE2N. Determined by X-ray diffraction at 2.1 Å resolution. Released 27 May 2020.

Method: X-ray diffraction
Resolution: 2.1 Å
Organisms: Legionella pneumophila, Homo sapiens
Chains: 3
Atoms: 4,894
Mol. weight: 69.93 kDa
Released: 27 May 2020

Explore 6P5B in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6P5B contains 39 α-helices and 25 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 27 helices, 12 β-strands

Element	Residues	Length	Sheet
α-helix	-2-1	4
α-helix	8-9	2
α-helix	10-24	15
α-helix	25-29	5
α-helix	33-44	12
α-helix	45-47	3
α-helix	54-64	11
α-helix	70-71	2
α-helix	74-96	23
α-helix	98-99	2
β-strand	100-101	2	1
α-helix	102-103	2
α-helix	107-110	4
β-strand	118-127	10	1
β-strand	132-135	4	2
α-helix	138-140	3
α-helix	147	1
β-strand	150-153	4	2
β-strand	158-161	4	2
β-strand	167-169	3	2
α-helix	174-183	10
α-helix	187-189	3
β-strand	191-193	3	2
α-helix	201-203	3
α-helix	205-221	17
β-strand	227-237	11	1
α-helix	243-245	3
β-strand	249-251	3	1
β-strand	254	1	3
β-strand	258	1	3
α-helix	260-265	6
α-helix	268-273	6
α-helix	277-289	13
α-helix	293-304	12
α-helix	306	1
α-helix	316-318	3
β-strand	324-332	9	1
α-helix	335-352	18
α-helix	358-380	23

Chain C: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	6-17	12
α-helix	19-20	2
β-strand	23-28	6	4
β-strand	31-40	10	4
α-helix	41-42	2
β-strand	51-57	7	4
α-helix	66-67	2
β-strand	68-71	4	4
β-strand	80	1	5
β-strand	86	1	5
α-helix	101-113	13
α-helix	115-117	3
α-helix	124-131	8
α-helix	133-147	15

Chain E: 4 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	2-7	6	6
β-strand	12-16	5	6
β-strand	22	1	7
α-helix	23-34	12
α-helix	38-40	3
β-strand	42-45	4	6
β-strand	48-49	2	6
α-helix	50-51	2
β-strand	55	1	7
α-helix	57-59	3
β-strand	66-70	5	6

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
MavC	A	protein	389	Legionella pneumophila	Q5ZTL4 (AlphaFold model)
Ubiquitin-conjugating enzyme E2 N	C	protein	152	Homo sapiens	P61088 (AlphaFold model)
Ubiquitin	E	protein	76	Homo sapiens	P0CG48 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>6P5B_1 MavC (chains A)
GPLGSMTTSKLEKTGLHVHEKIKHMVKNYGTMITGIPAEILGQNEAEISVGYVKKMGNMK
ENIAEVVRKSEMTQPTNSAGKASNEVCDLLLGTEGASEFEKSSYQVLSGDGSNLKGSLPN
KNLLVRVEMDRFNAPQKYQKIKREEFNPETAEKNKIYLLEDQLVYLDIFGKVIDLGQTSD
TCHRLFNAITTPFYQNYILYDEYIDPEESAEEAAMFEMGEIVKAKMKNIDCWTATHSFTI
FVPESDSEDTRTLYPYQAYWTSHTLQQWFSGDKDEKLSRLGIDGYIEKLALLGTTTDSKI
RSSIYGELFSPPGKEHVFCTGMNEKFSPLRVKFKVTEVNPEIALQNLEEVQEFIDTNYPG
ENAKDQCELYKIKAQEAMTKQLEMRLLIE

Sequence of entity 2 (C), FASTA

>6P5B_2 Ubiquitin-conjugating enzyme E2 N (chains C)
MAGLPRRIIKETQRLLAEPVPGIKAEPDESNARYFHVVIAGPQDSPFEGGTFKLELFLPE
EYPMAAPKVRFMTKIYHPNVDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDP
LANDVAEQWKTNEAQAIETARAWTRLYAMNNI

Sequence of entity 3 (E), FASTA

>6P5B_3 Ubiquitin (chains E)
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGG

Primary citation

Legionella effector MavC targets the Ube2N~Ub conjugate for noncanonical ubiquitination. Puvar, K., Iyer, S., Fu, J. et al. Nat Commun (2020) 11:2365-2365. DOI 10.1038/s41467-020-16211-x · PubMed

Other PDB entries of the same protein (UniProt Q5ZTL4 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6P5H 1.53 Å, Structure of MavC middle insertion domain
6ULH 1.97 Å, Structure of MavC in complex with its substrate in R3 spacegroup
6K3B 1.97 Å, Crystal structure of Lpg2147-Lpg2149 complex
5TSC 2.01 Å, The crystal structure of Lpg2147 from Legionella pneumophila
6UMS 2.34 Å, Crystal structure of MavC in complex with its substrate mimic in C222(1) space group
6KG6 2.39 Å, Crystal structure of MavC/UBE2N-Ub complex
7BXH 2.7 Å, MavC-Lpg2149 complex
7BXG 2.71 Å, MavC-UBE2N-Ub complex
6UMP 2.8 Å, Crystal structure of MavC in complex with substrate mimic in P65 space group
6KL4 2.85 Å, Crystal structure of MavC-UBE2N-Ub
6KFP 2.92 Å, Crystal structure of MavC ternary complex

Browse structure collections

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