6TWY: MAGI1_2

MAGI1_2 complexed with a phosphomimetic RSK1 peptide. Determined by X-ray diffraction at 2.3 Å resolution. Released 1 Apr 2020.

Method: X-ray diffraction
Resolution: 2.3 Å
Organism: Homo sapiens
Chains: 3
Atoms: 6,892
Mol. weight: 98.87 kDa
Ligands: CA, CIT
Released: 1 Apr 2020

Explore 6TWY in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

6TWY contains 48 α-helices and 17 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 24 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	463-465	3
β-strand	469-476	8	1
β-strand	478	1	2
β-strand	481	1	2
β-strand	484-488	5	1
β-strand	496-501	6	1
α-helix	506-510	5
β-strand	518-522	5	1
β-strand	526	1	1
α-helix	532-541	10
β-strand	547-554	8	1
α-helix	574-585	12
α-helix	592-599	8
α-helix	604-618	15
α-helix	622-629	8
α-helix	632-642	11
α-helix	645-656	12
α-helix	664-673	10
α-helix	676-690	15
α-helix	694-701	8
α-helix	704-714	11
α-helix	718-721	4
α-helix	727-739	13
α-helix	749-758	10
α-helix	761-774	14
α-helix	779-786	8
α-helix	789-803	15
α-helix	805-817	13
α-helix	824-834	11
α-helix	839-850	12
α-helix	854-861	8
α-helix	864-874	11

Chain B: 24 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	463-465	3
β-strand	469-476	8	3
β-strand	478	1	4
β-strand	481	1	4
β-strand	484-488	5	3
β-strand	496-501	6	3
α-helix	506-510	5
β-strand	518-522	5	3
β-strand	525-526	2	3
α-helix	532-541	10
α-helix	543	1
β-strand	547-554	8	3
α-helix	574-586	13
α-helix	592-599	8
α-helix	604-618	15
α-helix	622-629	8
α-helix	632-642	11
α-helix	645-656	12
α-helix	664-673	10
α-helix	676-690	15
α-helix	694-701	8
α-helix	704-714	11
α-helix	718-722	5
α-helix	727-740	14
α-helix	749-758	10
α-helix	761-771	11
α-helix	779-786	8
α-helix	789-817	29
α-helix	824-833	10
α-helix	839-850	12
α-helix	854-861	8
α-helix	864-874	11

Chain C: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	732-734	3	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1,Annexin A2	A, B	protein	427	Homo sapiens	P07355 (AlphaFold model), Q96QZ7 (AlphaFold model)
Phosphomimetic RSK1 peptide	C	protein	11	Homo sapiens	Q15418 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>6TWY_1 Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1,Annexin A2 (chains A, B)
GSMGKPFFTRNPSELKGKFIHTKLRKSSRGFGFTVVGGDEPDEFLQIKSLVLDGPAALDG
KMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLGSSAYGSVKAYTN
FDAERDALNIETAIKTKGVDEVTIVNILTNRSNEQRQDIAFAYQRRTKKELASALKSALS
GHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYK
TDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPK
WISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFAD
RLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALL
YLCGGDD

Sequence of entity 2 (C), FASTA

>6TWY_2 Phosphomimetic RSK1 peptide (chains C)
RRVRKLPETTL

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	11
CIT	Citric acid	C6 H8 O7	3

Water and common crystallization additives (GOL) are not listed.

Primary citation

Dual Specificity PDZ- and 14-3-3-Binding Motifs: A Structural and Interactomics Study. Gogl, G., Jane, P., Caillet-Saguy, C. et al. Structure (2020) 28:747-759.e3. DOI 10.1016/j.str.2020.03.010 · PubMed

Other PDB entries of the same protein (UniProt P07355 (AlphaFold model), which also has an AlphaFold model), best resolution first:

2HYV 1.42 Å, Human Annexin A2 with heparin hexasaccharide bound
1W7B 1.52 Å, Annexin A2: Does it induce membrane aggregation by a new multimeric state of the protein.
7QQM 1.6 Å, The PDZ domain of LRRC7 fused with ANXA2
7PC5 1.7 Å, The third PDZ domain of PDZD7 complexed with the PDZ-binding motif of EXOC4
7P73 1.85 Å, The PDZ domain of SYNJ2BP complexed with the PDZ-binding motif of HTLV1-TAX1
2HYU 1.86 Å, Human Annexin A2 with heparin tetrasaccharide bound
7ZVN 1.87 Å, Crystal structure of human Annexin A2 in complex with full phosphorothioate 5-10…
5LPX 1.9 Å, Crystal structure of PKC phosphorylation-mimicking mutant (S26E) Annexin A2
7P74 1.9 Å, The PDZ domain of SYNJ2BP complexed with the phosphorylated PDZ-binding motif of RSK1
7PC3 1.95 Å, The second PDZ domain of DLG1 complexed with the PDZ-binding motif of HTLV1-TAX1
7P70 2.0 Å, The PDZ-domain of SNTB1 complexed with the PDZ-binding motif of HPV35-E6
7PCB 2.0 Å, The PDZ domain of SNX27 fused with ANXA2

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