Cryo-EM structure of human GABA(B) receptor bound to the positive allosteric modulator rac-BHFF. Determined by electron microscopy at 4.5 Å resolution. Released 11 Nov 2020.
Explore 7CA3 in 3D Show helices and sheets RCSB PDB PDBe
7CA3 contains 59 α-helices and 52 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 170 | 1 | 1 |
| β-strand | 173-174 | 2 | 2 |
| β-strand | 175-176 | 2 | 3 |
| α-helix | 188-199 | 12 | |
| β-strand | 211 | 1 | 1 |
| β-strand | 216-217 | 2 | 3 |
| α-helix | 222-234 | 13 | |
| β-strand | 241-243 | 3 | 2 |
| α-helix | 247-257 | 11 | |
| β-strand | 263-265 | 3 | 2 |
| α-helix | 272-275 | 4 | |
| β-strand | 283-284 | 2 | 2 |
| α-helix | 288-289 | 2 | |
| α-helix | 290-292 | 3 | |
| α-helix | 293-303 | 11 | |
| β-strand | 311 | 1 | 4 |
| β-strand | 314 | 1 | 5 |
| α-helix | 317-331 | 15 | |
| β-strand | 343 | 1 | 5 |
| α-helix | 348-355 | 8 | |
| β-strand | 361-363 | 3 | 4 |
| α-helix | 368-379 | 12 | |
| β-strand | 389-391 | 3 | 4 |
| α-helix | 413-418 | 6 | |
| β-strand | 423-427 | 5 | 4 |
| α-helix | 443-452 | 10 | |
| α-helix | 468-484 | 17 | |
| α-helix | 504-515 | 12 | |
| β-strand | 517 | 1 | 6 |
| β-strand | 526 | 1 | 6 |
| β-strand | 536 | 1 | 7 |
| β-strand | 537-542 | 6 | 4 |
| β-strand | 547-548 | 2 | 4 |
| β-strand | 551-554 | 4 | 7 |
| β-strand | 559-562 | 4 | 7 |
| α-helix | 579-580 | 2 | |
| β-strand | 581 | 1 | 8 |
| β-strand | 584 | 1 | 9 |
| α-helix | 589-615 | 27 | |
| α-helix | 627-642 | 16 | |
| α-helix | 644-647 | 4 | |
| α-helix | 660-663 | 4 | |
| α-helix | 665-690 | 26 | |
| α-helix | 709-729 | 21 | |
| β-strand | 738-740 | 3 | 9 |
| β-strand | 754 | 1 | 8 |
| β-strand | 757-759 | 3 | 9 |
| α-helix | 769-790 | 22 | |
| α-helix | 803-825 | 23 | |
| α-helix | 830-832 | 3 | |
| α-helix | 833-845 | 13 | |
| α-helix | 849-859 | 11 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 55-56 | 2 | 10 |
| β-strand | 59-62 | 4 | 11 |
| α-helix | 71-76 | 6 | |
| α-helix | 78-91 | 14 | |
| β-strand | 98-99 | 2 | 10 |
| β-strand | 102-105 | 4 | 11 |
| α-helix | 110-122 | 13 | |
| β-strand | 129-132 | 4 | 11 |
| α-helix | 136-142 | 7 | |
| α-helix | 143-145 | 3 | |
| β-strand | 152-154 | 3 | 11 |
| α-helix | 161-164 | 4 | |
| β-strand | 172 | 1 | 11 |
| α-helix | 179-182 | 4 | |
| α-helix | 183-193 | 11 | |
| β-strand | 199 | 1 | 12 |
| β-strand | 202 | 1 | 13 |
| α-helix | 206-218 | 13 | |
| β-strand | 228 | 1 | 12 |
| α-helix | 240-244 | 5 | |
| β-strand | 250-252 | 3 | 14 |
| β-strand | 254 | 1 | 13 |
| α-helix | 257-259 | 3 | |
| α-helix | 260-268 | 9 | |
| β-strand | 278-282 | 5 | 14 |
| α-helix | 286 | 1 | |
| α-helix | 304-311 | 8 | |
| β-strand | 315-319 | 5 | 14 |
| α-helix | 335-345 | 11 | |
| α-helix | 350-352 | 3 | |
| α-helix | 356-373 | 18 | |
| α-helix | 378-384 | 7 | |
| α-helix | 393-403 | 11 | |
| β-strand | 408-410 | 3 | 15 |
| β-strand | 413-415 | 3 | 15 |
| β-strand | 417-418 | 2 | 16 |
| β-strand | 421-422 | 2 | 16 |
| α-helix | 423 | 1 | |
| β-strand | 426-431 | 6 | 14 |
| β-strand | 436-441 | 6 | 14 |
| β-strand | 450-451 | 2 | 14 |
| β-strand | 470-471 | 2 | 17 |
| β-strand | 474 | 1 | 18 |
| α-helix | 484-506 | 23 | |
| α-helix | 512-514 | 3 | |
| α-helix | 517-532 | 16 | |
| α-helix | 533-537 | 5 | |
| α-helix | 549-582 | 34 | |
| α-helix | 595-615 | 21 | |
| β-strand | 626-627 | 2 | 18 |
| β-strand | 640-641 | 2 | 17 |
| β-strand | 644-645 | 2 | 18 |
| α-helix | 658-678 | 21 | |
| α-helix | 683-685 | 3 | |
| α-helix | 689-711 | 23 | |
| α-helix | 717-732 | 16 | |
| α-helix | 733-737 | 5 | |
| α-helix | 738-740 | 3 | |
| α-helix | 742-745 | 4 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Gamma-aminobutyric acid type B receptor subunit 1 | A | protein | 771 | Homo sapiens | Q9UBS5 (AlphaFold model) |
| Gamma-aminobutyric acid type B receptor subunit 2 | B | protein | 822 | Homo sapiens | O75899 (AlphaFold model) |
>7CA3_1 Gamma-aminobutyric acid type B receptor subunit 1 (chains A) MRLLTALFAYFIVALILAFSVSAKSMSERRAVYIGALFPMSGGWPGGQACQPAVEMALED VNSRRDILPDYELKLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAAR MWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEV FTSTLDDLEERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEV YKERLFGKKYVWFLIGWYADNWFKIYDPSINCTVDEMTEAVEGHITTEIVMLNPANTRSI SNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALNKTSGGGGRSGVRLEDF NYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGYYDSTKD DLSWSKTDKWIGGSPPADQTLVIKTFRFLSQKLFISVSVLSSLGIVLAVVCLSFNIYNSH VRYIQNSQPNLNNLTAVGCSLALAAVFPLGLDGYHIGRNQFPFVCQARLWLLGLGFSLGY GSMFTKIWWVHTVFTKKEEKKEWRKTLEPWKLYATVGLLVGMDVLTLAIWQIVDPLHRTI ETFAKEEPKEDIDVSILPQLEHCSSRKMNTWLGIFYGYKGLLLLLGIFLAYETKSVSTEK INDHRAVGMAIYNVAVLCLITAPVTMILSSQQDAAFAFASLAIVFSSYITLVVLFVPKMR RLITRGEWQSEAQDTMKTGSSTNNNEEEKSRLLEKENRELEKSGRLEVLFQ
>7CA3_2 Gamma-aminobutyric acid type B receptor subunit 2 (chains B) MASPRSSGQPGPPPPPPPPPARLLLLLLLPLLLPLAPGAWGWARGAPRPPPSSPPLSIMG LMPLTKEVAKGSIGRGVLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDA IKYGPNHLMVFGGVCPSVTSIIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDN AVNPAILKLLKHYQWKRVGTLTQDVQRFSEVRNDLTGVLYGEDIEISDTESFSNDPCTSV KKLKGNDVRIILGQFDQNMAAKVFCCAYEENMYGSKYQWIIPGWYEPSWWEQVHTEANSS RCLRKNLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQYEREYNNKRSGVGPSKFHGYAYD GIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGRIILNAMNETNFFGVTGQVVFRNG ERMGTIKFTQFQDSREVKVGEYNAVADTLEIINDTIRFQGSEPPKDKTIILEQLRKISLP LYSILSALTILGMIMASAFLFFNIKNRNQKLIKMSSPYMNNLIILGGMLSYASIFLFGLD GSFVSEKTFETLCTVRTWILTVGYTTAFGAMFAKTWRVHAIFKNVKMKKKIIKDQKLLVI VGGMLLIDLCILICWQAVDPLRRTVEKYSMEPDPAGRDISIRPLLEHCENTHMTIWLGIV YAYKGLLMLFGCFLAWETRNVSIPALNDSKYIGMSVYNVGIMCIIGAAVSFLTRDQPNVQ FCIVALVIIFCSTITLCLVFVPKLITLRTNPDAATQNRRFQFTQNQKKEDSKTSTSVTSV NQASTSRSGRGGSENLYFQGGSGSGGDYKDDDDKDYKDDDDK
| ID | Name | Formula | Copies |
|---|---|---|---|
| FN0 | (3S)-5,7-ditert-butyl-3-oxidanyl-3-(trifluoromethyl)-1-benzofuran-2-one | C17 H21 F3 O3 | 1 |
| CLR | Cholesterol | C27 H46 O | 3 |
Structural Basis for Activation of the Heterodimeric GABA B Receptor. Kim, Y., Jeong, E., Jeong, J.H. et al. J Mol Biol (2020) 432:5966-5984. DOI 10.1016/j.jmb.2020.09.023 · PubMed
Other PDB entries of the same protein (UniProt Q9UBS5 (AlphaFold model), which also has an AlphaFold model), best resolution first:
MolViewer shows 7CA3 directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.