7CTG: Human Origin Recognition Complex, ORC1-5 State I
Human Origin Recognition Complex, ORC1-5 State I. Determined by electron microscopy at 5.0 Å resolution. Released 6 Jan 2021.
- Method
- Electron microscopy
- Resolution
- 5.0 Å
- Organism
- Homo sapiens
- Chains
- 5
- Atoms
- 14,504
- Mol. weight
- 347.74 kDa
- Ligands
- ATP
- Released
- 6 Jan 2021
Explore 7CTG in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7CTG contains 96 α-helices and 44 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain A: 5 helices, 4 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 762-768 | 7 | |
| α-helix | 772-788 | 17 | |
| β-strand | 793 | 1 | 1 |
| α-helix | 795-808 | 14 | |
| α-helix | 816-828 | 13 | |
| β-strand | 832-833 | 2 | 2 |
| β-strand | 845 | 1 | 1 |
| β-strand | 846-847 | 2 | 2 |
| α-helix | 851-859 | 9 | |
Chain B: 17 helices, 10 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 270-273 | 4 | |
| α-helix | 285-292 | 8 | |
| α-helix | 294-297 | 4 | |
| α-helix | 298-306 | 9 | |
| β-strand | 310-314 | 5 | 3 |
| α-helix | 320-330 | 11 | |
| β-strand | 335 | 1 | 4 |
| β-strand | 336-339 | 4 | 5 |
| α-helix | 348-357 | 10 | |
| α-helix | 369-382 | 14 | |
| β-strand | 388 | 1 | 4 |
| β-strand | 391-392 | 2 | 5 |
| α-helix | 405-413 | 9 | |
| β-strand | 421-424 | 4 | 3 |
| α-helix | 429-431 | 3 | |
| α-helix | 435-438 | 4 | |
| β-strand | 443-447 | 5 | 3 |
| α-helix | 455-460 | 6 | |
| α-helix | 474-483 | 10 | |
| α-helix | 486-500 | 15 | |
| β-strand | 511 | 1 | 6 |
| α-helix | 513-521 | 9 | |
| α-helix | 529-540 | 12 | |
| β-strand | 546-549 | 4 | 6 |
| α-helix | 554 | 1 | |
| β-strand | 555-558 | 4 | 6 |
| α-helix | 568-572 | 5 | |
Chain C: 30 helices, 15 β-strands
| Element | Residues | Length | Sheet |
|---|
| β-strand | 11-13 | 3 | 5 |
| α-helix | 40-65 | 26 | |
| α-helix | 67-82 | 16 | |
| β-strand | 101-102 | 2 | 7 |
| β-strand | 105-106 | 2 | 8 |
| α-helix | 114-128 | 15 | |
| β-strand | 133-136 | 4 | 9 |
| α-helix | 144-162 | 19 | |
| α-helix | 197-206 | 10 | |
| α-helix | 213-214 | 2 | |
| β-strand | 215 | 1 | 10 |
| β-strand | 216-219 | 4 | 9 |
| α-helix | 227-239 | 13 | |
| β-strand | 246 | 1 | 10 |
| β-strand | 248 | 1 | 7 |
| β-strand | 249-250 | 2 | 9 |
| β-strand | 251-252 | 2 | 8 |
| α-helix | 258-262 | 5 | |
| α-helix | 265-268 | 4 | |
| β-strand | 272-273 | 2 | 7 |
| β-strand | 276 | 1 | 8 |
| α-helix | 282-292 | 11 | |
| α-helix | 304-313 | 10 | |
| α-helix | 314-318 | 5 | |
| α-helix | 322-338 | 17 | |
| α-helix | 348-354 | 7 | |
| α-helix | 360-368 | 9 | |
| α-helix | 370-377 | 8 | |
| α-helix | 381-387 | 7 | |
| α-helix | 391-424 | 34 | |
| α-helix | 435-441 | 7 | |
| α-helix | 451-459 | 9 | |
| α-helix | 466-481 | 16 | |
| α-helix | 483-486 | 4 | |
| α-helix | 493-500 | 8 | |
| α-helix | 549-565 | 17 | |
| α-helix | 570-573 | 4 | |
| α-helix | 585-592 | 8 | |
| α-helix | 596-605 | 10 | |
| α-helix | 630-637 | 8 | |
| β-strand | 644 | 1 | 11 |
| α-helix | 646-662 | 17 | |
| α-helix | 670-690 | 21 | |
| β-strand | 695-696 | 2 | 11 |
| β-strand | 704-705 | 2 | 11 |
Chain D: 23 helices, 8 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 15-29 | 15 | |
| α-helix | 41-56 | 16 | |
| β-strand | 64-65 | 2 | 12 |
| α-helix | 73-86 | 14 | |
| β-strand | 95-99 | 5 | 12 |
| α-helix | 107-117 | 11 | |
| α-helix | 120-122 | 3 | |
| α-helix | 132-145 | 14 | |
| β-strand | 154-159 | 6 | 12 |
| α-helix | 161-164 | 4 | |
| α-helix | 171-182 | 12 | |
| β-strand | 189-192 | 4 | 12 |
| α-helix | 198-201 | 4 | |
| α-helix | 204-207 | 4 | |
| α-helix | 214 | 1 | |
| β-strand | 215 | 1 | 12 |
| α-helix | 216 | 1 | |
| α-helix | 223-234 | 12 | |
| α-helix | 243-258 | 16 | |
| α-helix | 260-272 | 13 | |
| α-helix | 278-288 | 11 | |
| α-helix | 299-308 | 10 | |
| α-helix | 315-319 | 5 | |
| α-helix | 323-336 | 14 | |
| β-strand | 344 | 1 | 13 |
| α-helix | 346-359 | 14 | |
| α-helix | 369-379 | 11 | |
| β-strand | 385-387 | 3 | 13 |
| β-strand | 402-404 | 3 | 13 |
| α-helix | 408-416 | 9 | |
| α-helix | 423-431 | 9 | |
Chain E: 21 helices, 7 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 12-22 | 11 | |
| β-strand | 32-36 | 5 | 14 |
| α-helix | 45-54 | 10 | |
| β-strand | 58-62 | 5 | 14 |
| α-helix | 69-80 | 12 | |
| α-helix | 99-109 | 11 | |
| β-strand | 120-125 | 6 | 14 |
| α-helix | 127-129 | 3 | |
| α-helix | 137-142 | 6 | |
| α-helix | 144-147 | 4 | |
| β-strand | 152-157 | 6 | 14 |
| α-helix | 162-165 | 4 | |
| α-helix | 173-174 | 2 | |
| β-strand | 175-178 | 4 | 14 |
| α-helix | 179-182 | 4 | |
| α-helix | 184-190 | 7 | |
| α-helix | 201-215 | 15 | |
| α-helix | 222-243 | 22 | |
| α-helix | 250-267 | 18 | |
| α-helix | 304-318 | 15 | |
| α-helix | 324-327 | 4 | |
| α-helix | 354-356 | 3 | |
| α-helix | 359-369 | 11 | |
| α-helix | 378-389 | 12 | |
| β-strand | 393-395 | 3 | 15 |
| β-strand | 407-409 | 3 | 15 |
| α-helix | 413-421 | 9 | |
| α-helix | 428-431 | 4 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Origin recognition complex subunit 1 | A | protein | 861 | Homo sapiens | Q13415 (AlphaFold model) |
| Origin recognition complex subunit 2 | B | protein | 577 | Homo sapiens | Q13416 (AlphaFold model) |
| Origin recognition complex subunit 3 | C | protein | 711 | Homo sapiens | Q9UBD5 (AlphaFold model) |
| Origin recognition complex subunit 4 | D | protein | 436 | Homo sapiens | O43929 (AlphaFold model) |
| Origin recognition complex subunit 5 | E | protein | 435 | Homo sapiens | O43913 |
Sequence of entity 1 (A), FASTA
>7CTG_1 Origin recognition complex subunit 1 (chains A)
MAHYPTRLKTRKTYSWVGRPLLDRKLHYQTYREMCVKTEGCSTEIHIQIGQFVLIEGDDD
ENPYVAKLLELFEDDSDPPPKKRARVQWFVRFCEVPACKRHLLGRKPGAQEIFWYDYPAC
DSNINAETIIGLVRVIPLAPKDVVPTNLKNEKTLFVKLSWNEKKFRPLSSELFAELNKPQ
ESAAKCQKPVRAKSKSAESPSWTPAEHVAKRIESRHSASKSRQTPTHPLTPRARKRLELG
NLGNPQMSQQTSCASLDSPGRIKRKVAFSEITSPSKRSQPDKLQTLSPALKAPEKTRETG
LSYTEDDKKASPEHRIILRTRIAASKTIDIREERTLTPISGGQRSSVVPSVILKPENIKK
RDAKEAKAQNEATSTPHRIRRKSSVLTMNRIRQQLRFLGNSKSDQEEKEILPAAEISDSS
SDEEEASTPPLPRRAPRTVSRNLRSSLKSSLHTLTKVPKKSLKPRTPRCAAPQIRSRSLA
AQEPASVLEEARLRLHVSAVPESLPCREQEFQDIYNFVESKLLDHTGGCMYISGVPGTGK
TATVHEVIRCLQQAAQANDVPPFQYIEVNGMKLTEPHQVYVQILQKLTGQKATANHAAEL
LAKQFCTRGSPQETTVLLVDELDLLWTHKQDIMYNLFDWPTHKEARLVVLAIANTMDLPE
RIMMNRVSSRLGLTRMCFQPYTYSQLQQILRSRLKHLKAFEDDAIQLVARKVAALSGDAR
RCLDICRRATEICEFSQQKPDSPGLVTIAHSMEAVDEMFSSSYITAIKNSSVLEQSFLRA
ILAEFRRSGLEEATFQQIYSQHVALCRMEGLPYPTMSETMAVCSHLGSCRLLLVEPSRND
LLLRVRLNVSQDDVLYALKDE
Sequence of entity 2 (B), FASTA
>7CTG_2 Origin recognition complex subunit 2 (chains B)
MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKLKKERAQLLVNPKKIIKKPEYDLEED
DQEVLKDQNYVEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEKMAKLASELAKTPQKS
VSFSLKNDPEITINVPQSSKGHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRSHSDSE
SEYSASNSEDDEGVAQEHEEDTNAVIFSQKIQAQNRVVSAPVGKETPSKRMKRDKTSDLV
EEYFEAHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVSPSFSAELKQLNQQYEKLFHK
WMLQLHLGFNIVLYGLGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKSVLNSITEEVL
DHMGTFRSILDQLDWIVNKFKEDSSLELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYL
IASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYTEETSYENSLLVKQSGSLPLSSLTHV
LRSLTPNARGIFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFLVNSDLTLRAQLTEFR
DHKLIRTKKGTDGVEYLLIPVDNGTLTDFLEKEEEEA
Sequence of entity 3 (C), FASTA
>7CTG_3 Origin recognition complex subunit 3 (chains C)
MATSSMSKGCFVFKPNSKKRKISLPIEDYFNKGKNEPEDSKLRFETYQLIWQQMKSENER
LQEELNKNLFDNLIEFLQKSHSGFQKNSRDLGGQIKLREIPTAALVLGVNVTDHDLTFGS
LTEALQNNVTPYVVSLQAKDCPDMKHFLQKLISQLMDCCVDIKSKEEESVHVTQRKTHYS
MDSLSSWYMTVTQKTDPKMLSKKRTTSSQWQSPPVVVILKDMESFATKVLQDFIIISSQH
LHEFPLILIFGIATSPIIIHRLLPHAVSSLLCIELFQSLSCKEHLTTVLDKLLLTTQFPF
KINEKVLQVLTNIFLYHDFSVQNFIKGLQLSLLEHFYSQPLSVLCCNLPEAKRRINFLSN
NQCENIRRLPSFRRYVEKQASEKQVALLTNERYLKEETQLLLENLHVYHMNYFLVLRCLH
KFTSSLPKYPLGRQIRELYCTCLEKNIWDSEEYASVLQLLRMLAKDELMTILEKCFKVFK
SYCENHLGSTAKRIEEFLAQFQSLDETKEEEDASGSQPKGLQKTDLYHLQKSLLEMKELR
RSKKQTKFEVLRENVVNFIDCLVREYLLPPETQPLHEVVYFSAAHALREHLNAAPRIALH
TALNNPYYYLKNEALKSEEGCIPNIAPDICIAYKLHLECSRLINLVDWSEAFATVVTAAE
KMDANSATSEEMNEIIHARFIRAVSELELLGFIKPTKQKTDHVARLTWGGC
Sequence of entity 4 (D), FASTA
>7CTG_4 Origin recognition complex subunit 4 (chains D)
MSSRKSKSNSLIHTECLSQVQRILRERFCRQSPHSNLFGVQVQYKHLSELLKRTALHGES
NSVLIIGPRGSGKTMLINHALKELMEIEEVSENVLQVHLNGLLQINDKIALKEITRQLNL
ENVVGDKVFGSFAENLSFLLEALKKGDRTSSCPVIFILDEFDLFAHHKNQTLLYNLFDIS
QSAQTPIAVIGLTCRLDILELLEKRVKSRFSHRQIHLMNSFGFPQYVKIFKEQLSLPAEF
PDKVFAEKWNENVQYLSEDRSVQEVLQKHFNISKNLRSLHMLLMLALNRVTASHPFMTAV
DLMEASQLCSMDSKANIVHGLSVLEICLIIAMKHLNDIYEEEPFNFQMVYNEFQKFVQRK
AHSVYNFEKPVVMKAFEHLQQLELIKPMERTSGNSEREYQLMKLLLDNTQIMNALQKYPN
CPTDVRQWATSSLSWL
Sequence of entity 5 (E), FASTA
>7CTG_5 Origin recognition complex subunit 5 (chains E)
MPHLENVVLCRESQVSILQSLFGERHHFSFPSIFIYGHTASGKTYVTQTLLKTLELPHVF
VNCVECFTLRLLLEQILNKLNHLSSSEDGCSTEITCETFNDFVRLFKQVTTAENLKDQTV
YIVLDKAEYLRDMEANLLPGFLRLQELADRNVTVLFLSEIVWEKFRPNTGCFEPFVLYFP
DYSIGNLQKILSHDHPPEYSADFYAAYINILLGVFYTVCRDLKELRHLAVLNFPKYCEPV
VKGEASERDTRKLWRNIEPHLKKAMQTVYLREISSSQWEKLQKDDTDPGQLKGLSAHTHV
ELPYYSKFILIAAYLASYNPARTDKRFFLKHHGKIKKTNFLKKHEKTSNHLLGPKPFPLD
RLLAILYSIVDSRVAPTANIFSQITSLVTLQLLTLVGHDDQLDGPKYKCTVSLDFIRAIA
RTVNFDIIKYLYDFL
Ligands and cofactors
| ID | Name | Formula | Copies |
|---|
| ATP | Adenosine-5'-triphosphate | C10 H16 N5 O13 P3 | 2 |
Primary citation
Structural insight into the assembly and conformational activation of human origin recognition complex. Cheng, J., Li, N., Wang, X. et al. Cell Discov (2020) 6:88-88. DOI 10.1038/s41421-020-00232-3 · PubMed
Other PDB entries of the same protein (UniProt Q13415 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 6P3W 2.54 Å, Crystal structure of the Cyclin A-CDK2-ORC1 complex
- 11RL 2.8 Å, CryoEM structure of the human origin recognition complex with DNA and CDC6 protein
- 7JPO 3.2 Å, ORC-O1AAA: Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC2 WH
- 5UJ7 3.39 Å, Structure of the active form of human Origin Recognition Complex ATPase motor module,…
- 8S0D 3.6 Å, H. sapiens MCM bound to double stranded DNA and ORC1-6
- 7JPP 3.7 Å, ORC-O2WH: Human Origin Recognition Complex (ORC) with dynamic/unresolved ORC1 AAA+ domain
- 8S0E 3.8 Å, H. sapiens OCCM bound to double stranded DNA
- 7JPR 4.0 Å, ORC-OPEN: Human Origin Recognition Complex (ORC) in an open conformation
- 8S0C 4.0 Å, H. sapiens ORC1-5 bound to double stranded DNA as part of the MCM-ORC complex
- 8S0F 4.1 Å, H. sapiens OC1M bound to double stranded DNA
- 7JPS 4.4 Å, ORC-DNA: Human Origin Recognition Complex (ORC) with DNA bound in the core
- 7CTF 4.8 Å, Human origin recognition complex 1-5 State II
Browse structure collections
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