Cryo-EM structure of human GABA(B) receptor bound to the antagonist CGP54626. Determined by electron microscopy at 3.52 Å resolution. Released 11 Nov 2020.
Explore 7CUM in 3D Show helices and sheets RCSB PDB PDBe
7CUM contains 52 α-helices and 48 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 168-176 | 9 | 1 |
| α-helix | 185-200 | 16 | |
| β-strand | 209-217 | 9 | 1 |
| α-helix | 222-233 | 12 | |
| α-helix | 238 | 1 | |
| β-strand | 239-243 | 5 | 1 |
| α-helix | 247-256 | 10 | |
| β-strand | 263-266 | 4 | 1 |
| α-helix | 272-274 | 3 | |
| β-strand | 283-285 | 3 | 1 |
| α-helix | 288-289 | 2 | |
| α-helix | 293-302 | 10 | |
| β-strand | 308-311 | 4 | 2 |
| β-strand | 314 | 1 | 3 |
| α-helix | 317-330 | 14 | |
| β-strand | 336-340 | 5 | 2 |
| β-strand | 343 | 1 | 3 |
| α-helix | 348-357 | 10 | |
| β-strand | 361 | 1 | 4 |
| β-strand | 362-363 | 2 | 2 |
| α-helix | 368-381 | 14 | |
| β-strand | 389 | 1 | 4 |
| β-strand | 391-393 | 3 | 5 |
| β-strand | 423-427 | 5 | 5 |
| α-helix | 443-453 | 11 | |
| α-helix | 467-480 | 14 | |
| α-helix | 504-514 | 11 | |
| β-strand | 517-520 | 4 | 6 |
| β-strand | 523-526 | 4 | 6 |
| β-strand | 527 | 1 | 7 |
| β-strand | 533 | 1 | 7 |
| β-strand | 537-542 | 6 | 5 |
| β-strand | 547-554 | 8 | 5 |
| β-strand | 559-562 | 4 | 5 |
| α-helix | 573-575 | 3 | |
| α-helix | 578-579 | 2 | |
| β-strand | 580-584 | 5 | 8 |
| α-helix | 590-615 | 26 | |
| α-helix | 630-642 | 13 | |
| α-helix | 659-692 | 34 | |
| α-helix | 707-730 | 24 | |
| β-strand | 735-740 | 6 | 8 |
| β-strand | 744 | 1 | 8 |
| β-strand | 753-762 | 10 | 8 |
| α-helix | 766-769 | 4 | |
| α-helix | 771-790 | 20 | |
| α-helix | 805-819 | 15 | |
| α-helix | 822-825 | 4 | |
| α-helix | 832-858 | 27 |
| Element | Residues | Length | Sheet |
|---|---|---|---|
| β-strand | 55-61 | 7 | 9 |
| α-helix | 71-76 | 6 | |
| α-helix | 78-91 | 14 | |
| β-strand | 98-104 | 7 | 9 |
| α-helix | 110-122 | 13 | |
| β-strand | 127-132 | 6 | 9 |
| α-helix | 136-145 | 10 | |
| α-helix | 146-148 | 3 | |
| β-strand | 153-155 | 3 | 9 |
| α-helix | 161-164 | 4 | |
| β-strand | 172-174 | 3 | 9 |
| α-helix | 180-182 | 3 | |
| α-helix | 183-193 | 11 | |
| β-strand | 197-202 | 6 | 10 |
| β-strand | 203 | 1 | 11 |
| α-helix | 206-219 | 14 | |
| β-strand | 225-229 | 5 | 10 |
| β-strand | 232 | 1 | 11 |
| α-helix | 237-246 | 10 | |
| β-strand | 250-254 | 5 | 10 |
| α-helix | 257-270 | 14 | |
| β-strand | 278-282 | 5 | 10 |
| α-helix | 286 | 1 | |
| α-helix | 289-291 | 3 | |
| α-helix | 304-310 | 7 | |
| β-strand | 315-319 | 5 | 10 |
| α-helix | 335-345 | 11 | |
| α-helix | 355-376 | 22 | |
| α-helix | 394-404 | 11 | |
| β-strand | 407-410 | 4 | 12 |
| β-strand | 413-416 | 4 | 12 |
| β-strand | 417-418 | 2 | 13 |
| β-strand | 421-422 | 2 | 13 |
| β-strand | 425-432 | 8 | 10 |
| β-strand | 435-443 | 9 | 10 |
| β-strand | 448-450 | 3 | 10 |
| α-helix | 463-465 | 3 | |
| α-helix | 468-469 | 2 | |
| β-strand | 470-471 | 2 | 14 |
| α-helix | 479-505 | 27 | |
| α-helix | 513-515 | 3 | |
| α-helix | 517-531 | 15 | |
| α-helix | 533-536 | 4 | |
| α-helix | 549-583 | 35 | |
| α-helix | 599-618 | 20 | |
| β-strand | 623-627 | 5 | 15 |
| β-strand | 640-641 | 2 | 14 |
| β-strand | 644-648 | 5 | 15 |
| α-helix | 658-678 | 21 | |
| α-helix | 690-712 | 23 | |
| α-helix | 717-748 | 32 |
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|---|---|---|---|---|
| Gamma-aminobutyric acid type B receptor subunit 1 | A | protein | 771 | Homo sapiens | Q9UBS5 (AlphaFold model) |
| Gamma-aminobutyric acid type B receptor subunit 2 | B | protein | 822 | Homo sapiens | O75899 (AlphaFold model) |
>7CUM_1 Gamma-aminobutyric acid type B receptor subunit 1 (chains A) MRLLTALFAYFIVALILAFSVSAKSMSERRAVYIGALFPMSGGWPGGQACQPAVEMALED VNSRRDILPDYELKLIHHDSKCDPGQATKYLYELLYNDPIKIILMPGCSSVSTLVAEAAR MWNLIVLSYGSSSPALSNRQRFPTFFRTHPSATLHNPTRVKLFEKWGWKKIATIQQTTEV FTSTLDDLEERVKEAGIEITFRQSFFSDPAVPVKNLKRQDARIIVGLFYETEARKVFCEV YKERLFGKKYVWFLIGWYADNWFKIYDPSINCTVDEMTEAVEGHITTEIVMLNPANTRSI SNMTSQEFVEKLTKRLKRHPEETGGFQEAPLAYDAIWALALALNKTSGGGGRSGVRLEDF NYNNQTITDQIYRAMNSSSFEGVSGHVVFDASGSRMAWTLIEQLQGGSYKKIGYYDSTKD DLSWSKTDKWIGGSPPADQTLVIKTFRFLSQKLFISVSVLSSLGIVLAVVCLSFNIYNSH VRYIQNSQPNLNNLTAVGCSLALAAVFPLGLDGYHIGRNQFPFVCQARLWLLGLGFSLGY GSMFTKIWWVHTVFTKKEEKKEWRKTLEPWKLYATVGLLVGMDVLTLAIWQIVDPLHRTI ETFAKEEPKEDIDVSILPQLEHCSSRKMNTWLGIFYGYKGLLLLLGIFLAYETKSVSTEK INDHRAVGMAIYNVAVLCLITAPVTMILSSQQDAAFAFASLAIVFSSYITLVVLFVPKMR RLITRGEWQSEAQDTMKTGSSTNNNEEEKSRLLEKENRELEKSGRLEVLFQ
>7CUM_2 Gamma-aminobutyric acid type B receptor subunit 2 (chains B) MASPRSSGQPGPPPPPPPPPARLLLLLLLPLLLPLAPGAWGWARGAPRPPPSSPPLSIMG LMPLTKEVAKGSIGRGVLPAVELAIEQIRNESLLRPYFLDLRLYDTECDNAKGLKAFYDA IKYGPNHLMVFGGVCPSVTSIIAESLQGWNLVQLSFAATTPVLADKKKYPYFFRTVPSDN AVNPAILKLLKHYQWKRVGTLTQDVQRFSEVRNDLTGVLYGEDIEISDTESFSNDPCTSV KKLKGNDVRIILGQFDQNMAAKVFCCAYEENMYGSKYQWIIPGWYEPSWWEQVHTEANSS RCLRKNLLAAMEGYIGVDFEPLSSKQIKTISGKTPQQYEREYNNKRSGVGPSKFHGYAYD GIWVIAKTLQRAMETLHASSRHQRIQDFNYTDHTLGRIILNAMNETNFFGVTGQVVFRNG ERMGTIKFTQFQDSREVKVGEYNAVADTLEIINDTIRFQGSEPPKDKTIILEQLRKISLP LYSILSALTILGMIMASAFLFFNIKNRNQKLIKMSSPYMNNLIILGGMLSYASIFLFGLD GSFVSEKTFETLCTVRTWILTVGYTTAFGAMFAKTWRVHAIFKNVKMKKKIIKDQKLLVI VGGMLLIDLCILICWQAVDPLRRTVEKYSMEPDPAGRDISIRPLLEHCENTHMTIWLGIV YAYKGLLMLFGCFLAWETRNVSIPALNDSKYIGMSVYNVGIMCIIGAAVSFLTRDQPNVQ FCIVALVIIFCSTITLCLVFVPKLITLRTNPDAATQNRRFQFTQNQKKEDSKTSTSVTSV NQASTSRSGRGGSENLYFQGGSGSGGDYKDDDDKDYKDDDDK
| ID | Name | Formula | Copies |
|---|---|---|---|
| 2BV | (R)-(cyclohexylmethyl)[(2S)-3-{[(1S)-1-(3,4-dichlorophenyl)ethyl]amino}-2-hydro… | C18 H28 Cl2 N O3 P | 1 |
| CLR | Cholesterol | C27 H46 O | 16 |
Water and common crystallization additives (UNL) are not listed.
Structural Basis for Activation of the Heterodimeric GABA B Receptor. Kim, Y., Jeong, E., Jeong, J.H. et al. J Mol Biol (2020) 432:5966-5984. DOI 10.1016/j.jmb.2020.09.023 · PubMed
Other PDB entries of the same protein (UniProt Q9UBS5 (AlphaFold model), which also has an AlphaFold model), best resolution first:
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