7DVQ: Activated Human Minor Spliceosome

Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex). Determined by electron microscopy at 2.89 Å resolution. Released 31 Mar 2021.

Method: Electron microscopy
Resolution: 2.89 Å
Organism: Homo sapiens
Chains: 49
Atoms: 99,906
Mol. weight: 2918.38 kDa
Ligands: IHP, GTP, MG, G5J
Released: 31 Mar 2021

Explore 7DVQ in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7DVQ contains 628 α-helices and 555 β-strands across 45 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 0: 5 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	6-9	4
α-helix	16-17	2
β-strand	57	1	106
α-helix	63	1
β-strand	64	1	106
α-helix	65	1
α-helix	74-80	7
β-strand	82	1	107
β-strand	89	1	107
β-strand	96	1	101

Chain 1: 76 helices, 5 β-strands

Element	Residues	Length	Sheet
α-helix	88-92	5
α-helix	94-95	2
α-helix	103-106	4
α-helix	109-111	3
α-helix	120-124	5
β-strand	128	1	113
β-strand	132	1	114
α-helix	137-140	4
α-helix	141-144	4
β-strand	150	1	114
α-helix	152-175	24
α-helix	401-404	4
α-helix	405-407	3
β-strand	412-414	3	23
α-helix	415-417	3
α-helix	446-449	4
α-helix	452-455	4
α-helix	469-471	3
α-helix	476-478	3
α-helix	489-506	18
α-helix	509-521	13
α-helix	523-526	4
α-helix	528-539	12
α-helix	546-562	17
α-helix	568-570	3
α-helix	571-578	8
α-helix	579-583	5
α-helix	587-604	18
α-helix	606-613	8
α-helix	615-617	3
α-helix	622-639	18
α-helix	641-652	12
α-helix	658-675	18
α-helix	676-682	7
α-helix	683-690	8
α-helix	691-693	3
α-helix	699-715	17
α-helix	728-737	10
α-helix	742-756	15
α-helix	759-776	18
α-helix	782-797	16
α-helix	803-805	3
α-helix	806-810	5
α-helix	811-817	7
α-helix	827-844	18
α-helix	846-853	8
α-helix	854-858	5
α-helix	862-878	17
α-helix	886-900	15
α-helix	909-921	13
α-helix	922-928	7
α-helix	929-939	11
β-strand	942	1	12
α-helix	945-957	13
α-helix	959-964	6
α-helix	968-980	13
α-helix	981-983	3
α-helix	987-1004	18
α-helix	1013-1020	8
α-helix	1021-1025	5
α-helix	1029-1045	17
α-helix	1047-1049	3
α-helix	1052-1061	10
α-helix	1065-1067	3
α-helix	1071-1088	18
α-helix	1090-1099	10
α-helix	1100-1102	3
α-helix	1106-1122	17
α-helix	1125-1134	10
α-helix	1135-1137	3
α-helix	1141-1158	18
α-helix	1159-1164	6
α-helix	1166-1177	12
α-helix	1182-1198	17
α-helix	1205-1215	11
α-helix	1216-1220	5
α-helix	1224-1241	18
α-helix	1243-1250	8
α-helix	1251-1253	3
α-helix	1259-1275	17
α-helix	1279-1282	4

Chain 2: 14 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	462-468	7
α-helix	479-481	3
α-helix	485-492	8
α-helix	499-501	3
α-helix	502-504	3
α-helix	509	1
α-helix	514-518	5
α-helix	519-522	4
α-helix	526-529	4
α-helix	533-543	11
α-helix	549-554	6
α-helix	568-576	9
α-helix	633-639	7
β-strand	651	1	115
β-strand	655	1	115
α-helix	692-702	11

Chain 3: 21 helices, 103 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	116
β-strand	16-20	5	117
β-strand	29-33	5	117
β-strand	37-43	7	117
β-strand	50-57	8	117
β-strand	62-68	7	118
β-strand	77-82	6	118
β-strand	86-93	8	118
β-strand	98-106	9	118
β-strand	120-123	4	119
β-strand	129-133	5	119
β-strand	136	1	120
β-strand	139-143	5	119
β-strand	144-146	3	121
β-strand	152-154	3	121
α-helix	156-157	2
β-strand	158-160	3	119
β-strand	165-173	9	120
β-strand	181-188	8	120
α-helix	192-194	3
α-helix	199-203	5
β-strand	207-214	8	120
β-strand	219-227	9	120
β-strand	232-237	6	122
α-helix	238-239	2
β-strand	248-252	5	122
β-strand	255-259	5	122
α-helix	264-266	3
β-strand	267-270	4	122
α-helix	271-272	2
β-strand	273-274	2	123
β-strand	287-294	8	124
β-strand	299-305	7	124
β-strand	309-318	10	124
β-strand	321-332	12	124
β-strand	336-342	7	125
β-strand	346-351	6	125
β-strand	356-361	6	125
β-strand	373-374	2	124
β-strand	386-387	2	123
β-strand	395-401	7	125
β-strand	407-413	7	126
β-strand	422-427	6	126
α-helix	430-432	3
β-strand	434-440	7	126
β-strand	442	1	127
β-strand	445-450	6	128
β-strand	456-462	7	129
β-strand	473-478	6	129
β-strand	482-486	5	129
β-strand	490-493	4	129
β-strand	507-509	3	130
β-strand	516-520	5	130
β-strand	523-527	5	130
β-strand	533-537	5	130
α-helix	538	1
β-strand	544-549	6	131
β-strand	553-558	6	131
β-strand	562-568	7	131
β-strand	574-576	3	131
β-strand	581	1	131
β-strand	587-592	6	132
α-helix	593-596	4
β-strand	599	1	44
β-strand	604-609	6	132
β-strand	613-619	7	132
β-strand	624-632	9	132
β-strand	637-645	9	133
α-helix	661	1
β-strand	662-669	8	133
β-strand	673-678	6	133
β-strand	689-693	5	133
β-strand	700-703	4	134
β-strand	706	1	135
β-strand	709	1	135
β-strand	712-715	4	134
β-strand	719-725	7	134
β-strand	728-732	5	134
β-strand	734	1	127
β-strand	741-745	5	128
β-strand	753-757	5	128
β-strand	762-766	5	128
β-strand	774-780	7	126
β-strand	784-790	7	136
β-strand	797-804	8	136
α-helix	809-826	18
α-helix	835-844	10
α-helix	849-852	4
β-strand	862-869	8	136
β-strand	876-881	6	136
β-strand	886-895	10	137
β-strand	903-911	9	137
β-strand	914	1	138
β-strand	919	1	138
β-strand	922-931	10	137
β-strand	936-945	10	137
β-strand	949-955	7	139
β-strand	958-963	6	139
β-strand	966-972	7	139
β-strand	977-983	7	139
β-strand	990-996	7	140
β-strand	999-1004	6	140
β-strand	1007	1	141
β-strand	1009-1015	7	140
α-helix	1016-1018	3
β-strand	1020-1026	7	140
β-strand	1031	1	141
β-strand	1033-1038	6	142
β-strand	1043-1048	6	142
β-strand	1052-1057	6	142
β-strand	1087-1088	2	140
α-helix	1089	1
β-strand	1090-1096	7	142
β-strand	1101-1108	8	116
β-strand	1115-1121	7	116
β-strand	1126-1131	6	116
α-helix	1135-1151	17
α-helix	1160-1163	4
β-strand	1171	1	142
β-strand	1173-1175	3	116
α-helix	1176-1179	4
α-helix	1180-1184	5
α-helix	1187-1196	10
α-helix	1201-1215	15

Chain 4: 3 helices, 8 β-strands

Element	Residues	Length	Sheet
β-strand	15-17	3	20
α-helix	26-34	9
β-strand	41-43	3	20
α-helix	45-46	2
β-strand	47	1	21
β-strand	52	1	21
β-strand	57-60	4	20
α-helix	64-76	13
β-strand	79	1	22
β-strand	82	1	22
β-strand	87-88	2	20

Chain 5: 4 helices, 7 β-strands

Element	Residues	Length	Sheet
β-strand	20-24	5	23
α-helix	26-27	2
α-helix	32-39	8
β-strand	45-51	7	23
β-strand	60-64	5	23
α-helix	67-77	11
β-strand	81-82	2	24
β-strand	85-86	2	24
β-strand	88-91	4	23
β-strand	96	1	113
α-helix	103-117	15

Chain 6: 7 helices, 11 β-strands

Element	Residues	Length	Sheet
β-strand	10	1	34
β-strand	18-19	2	35
α-helix	20	1
β-strand	21-22	2	36
β-strand	39-42	4	37
β-strand	44-45	2	35
α-helix	47-50	4
α-helix	52-54	3
β-strand	57	1	38
β-strand	58	1	36
β-strand	64	1	38
α-helix	65	1
β-strand	67-68	2	36
β-strand	70-72	3	37
α-helix	73-77	5
α-helix	80-82	3
β-strand	88	1	34
α-helix	92-105	14

Chain 7: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	2-17	16
α-helix	28-45	18
α-helix	47-56	10
α-helix	61-71	11

35 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Pre-mRNA-processing-splicing factor 8	A	protein	2335	Homo sapiens	Q6P2Q9 (AlphaFold model)
U5 snRNA	B	RNA	117	Homo sapiens
116 kDa U5 small nuclear ribonucleoprotein component	C	protein	972	Homo sapiens	Q15029 (AlphaFold model)
U5 small nuclear ribonucleoprotein 200 kDa helicase	D	protein	2136	Homo sapiens	O75643 (AlphaFold model)
U5 small nuclear ribonucleoprotein 40 kDa protein	E	protein	357	Homo sapiens	Q96DI7 (AlphaFold model)
Small nuclear ribonucleoprotein Sm D3	a, h	protein	126	Homo sapiens	P62318
Small nuclear ribonucleoprotein-associated proteins B and B'	b, i	protein	240	Homo sapiens	P14678
Small nuclear ribonucleoprotein Sm D1	c, j	protein	119	Homo sapiens	P62314
Small nuclear ribonucleoprotein Sm D2	d, k	protein	118	Homo sapiens	P62316
Small nuclear ribonucleoprotein F	f, m	protein	86	Homo sapiens	P62306
Small nuclear ribonucleoprotein E	e, l	protein	92	Homo sapiens	P62304
Small nuclear ribonucleoprotein G	g, n	protein	76	Homo sapiens	P62308

30 more molecules are not listed.

Sequence of entity 1 (A), FASTA

>7DVQ_1 Pre-mRNA-processing-splicing factor 8 (chains A)
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKED
MPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLY
HITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYAD
NILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYR
LANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDIN
KIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPIS
HRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRR
ALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATK
FFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRF
GNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMR
QIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQF
EGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCW
KANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLT
RLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLI
LALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMD
LYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGIN
NLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYK
DMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATE
AAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARM
RLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKC
RTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIV
NKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPK
ELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFI
DSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRT
DFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEG
LFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGI
FMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSY
KMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYA
RAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPAL
YVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTT
KPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQ
IIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLY
DDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVE
VQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLT
ATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKET
GYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLP
GQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTP
GSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWN
YNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Sequence of entity 2 (B), FASTA

>7DVQ_2 U5 snRNA (chains B)
AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGG
AGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGGCCUUGCUUUGGCAAGGCUA

Sequence of entity 3 (C), FASTA

>7DVQ_3 116 kDa U5 small nuclear ribonucleoprotein component (chains C)
MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLH
EDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLA
DLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVG
IKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTE
RLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLIL
SPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKA
PTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVC
KKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTK
MYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIE
VNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSEL
PKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVV
TFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQT
KYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLC
DELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPA
DCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSV
FHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLE
LAKQDVVLNYPM

Sequence of entity 4 (D), FASTA

>7DVQ_4 U5 small nuclear ribonucleoprotein 200 kDa helicase (chains D)
MADVTARSLQYEYKANSNLVLQADRSLIDRTRRDEPTGEVLSLVGKLEGTRMGDKAQRTK
PQMQEERRAKRRKRDEDRHDINKMKGYTLLSEGIDEMVGIIYKPKTKETRETYEVLLSFI
QAALGDQPRDILCGAADEVLAVLKNEKLRDKERRKEIDLLLGQTDDTRYHVLVNLGKKIT
DYGGDKEIQNMDDNIDETYGVNVQFESDEEEGDEDVYGEVREEASDDDMEGDEAVVRCTL
SANLVASGELMSSKKKDLHPRDIDAFWLQRQLSRFYDDAIVSQKKADEVLEILKTASDDR
ECENQLVLLLGFNTFDFIKVLRQHRMMILYCTLLASAQSEAEKERIMGKMEADPELSKFL
YQLHETEKEDLIREERSRRERVRQSRMDTDLETMDLDQGGEALAPRQVLDLEDLVFTQGS
HFMANKRCQLPDGSFRRQRKGYEEVHVPALKPKPFGSEEQLLPVEKLPKYAQAGFEGFKT
LNRIQSKLYRAALETDENLLLCAPTGAGKTNVALMCMLREIGKHINMDGTINVDDFKIIY
IAPMRSLVQEMVGSFGKRLATYGITVAELTGDHQLCKEEISATQIIVCTPEKWDIITRKG
GERTYTQLVRLIILDEIHLLHDDRGPVLEALVARAIRNIEMTQEDVRLIGLSATLPNYED
VATFLRVDPAKGLFYFDNSFRPVPLEQTYVGITEKKAIKRFQIMNEIVYEKIMEHAGKNQ
VLVFVHSRKETGKTARAIRDMCLEKDTLGLFLREGSASTEVLRTEAEQCKNLELKDLLPY
GFAIHHAGMTRVDRTLVEDLFADKHIQVLVSTATLAWGVNLPAHTVIIKGTQVYSPEKGR
WTELGALDILQMLGRAGRPQYDTKGEGILITSHGELQYYLSLLNQQLPIESQMVSKLPDM
LNAEIVLGNVQNAKDAVNWLGYAYLYIRMLRSPTLYGISHDDLKGDPLLDQRRLDLVHTA
ALMLDKNNLVKYDKKTGNFQVTELGRIASHYYITNDTVQTYNQLLKPTLSEIELFRVFSL
SSEFKNITVREEEKLELQKLLERVPIPVKESIEEPSAKINVLLQAFISQLKLEGFALMAD
MVYVTQSAGRLMRAIFEIVLNRGWAQLTDKTLNLCKMIDKRMWQSMCPLRQFRKLPEEVV
KKIEKKNFPFERLYDLNHNEIGELIRMPKMGKTIHKYVHLFPKLELSVHLQPITRSTLKV
ELTITPDFQWDEKVHGSSEAFWILVEDVDSEVILHHEYFLLKAKYAQDEHLITFFVPVFE
PLPPQYFIRVVSDRWLSCETQLPVSFRHLILPEKYPPPTELLDLQPLPVSALRNSAFESL
YQDKFPFFNPIQTQVFNTVYNSDDNVFVGAPTGSGKTICAEFAILRMLLQSSEGRCVYIT
PMEALAEQVYMDWYEKFQDRLNKKVVLLTGETSTDLKLLGKGNIIISTPEKWDILSRRWK
QRKNVQNINLFVVDEVHLIGGENGPVLEVICSRMRYISSQIERPIRIVALSSSLSNAKDV
AHWLGCSATSTFNFHPNVRPVPLELHIQGFNISHTQTRLLSMAKPVYHAITKHSPKKPVI
VFVPSRKQTRLTAIDILTTCAADIQRQRFLHCTEKDLIPYLEKLSDSTLKETLLNGVGYL
HEGLSPMERRLVEQLFSSGAIQVVVASRSLCWGMNVAAHLVIIMDTQYYNGKIHAYVDYP
IYDVLQMVGHANRPLQDDEGRCVIMCQGSKKDFFKKFLYEPLPVESHLDHCMHDHFNAEI
VTKTIENKQDAVDYLTWTFLYRRMTQNPNYYNLQGISHRHLSDHLSELVEQTLSDLEQSK
CISIEDEMDVAPLNLGMIAAYYYINYTTIELFSMSLNAKTKVRGLIEIISNAAEYENIPI
RHHEDNLLRQLAQKVPHKLNNPKFNDPHVKTNLLLQAHLSRMQLSAELQSDTEEILSKAI
RLIQACVDVLSSNGWLSPALAAMELAQMVTQAMWSKDSYLKQLPHFTSEHIKRCTDKGVE
SVFDIMEMEDEERNALLQLTDSQIADVARFCNRYPNIELSYEVVDKDSIRSGGPVVVLVQ
LEREEEVTGPVIAPLFPQKREEGWWVVIGDAKSNSLISIKRLTLQQKAKVKLDFVAPATG
AHNYTLYFMSDAYMGCDQEYKFSVDVKEAETDSDSD

Sequence of entity 5 (E), FASTA

>7DVQ_5 U5 small nuclear ribonucleoprotein 40 kDa protein (chains E)
MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIM
LLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTD
GSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWD
IRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTG
LSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKI
AAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ

Sequence of entity 6 (a, h), FASTA

>7DVQ_6 Small nuclear ribonucleoprotein Sm D3 (chains a, h)
MSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQ
VYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRGN
IFQKRR

Sequence of entity 7 (b, i), FASTA

>7DVQ_7 Small nuclear ribonucleoprotein-associated proteins B and B' (chains b, i)
MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAE
REEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPM
PQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMG
RGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGPPPPGMRPPRP

Sequence of entity 8 (c, j), FASTA

>7DVQ_8 Small nuclear ribonucleoprotein Sm D1 (chains c, j)
MKLVRFLMKLSHETVTIELKNGTQVHGTITGVDVSMNTHLKAVKMTLKNREPVQLETLSI
RGNNIRYFILPDSLPLDTLLVDVEPKVKSKKREAVAGRGRGRGRGRGRGRGRGRGGPRR

Sequence of entity 9 (d, k), FASTA

>7DVQ_9 Small nuclear ribonucleoprotein Sm D2 (chains d, k)
MSLLNKPKSEMTPEELQKREEEEFNTGPLSVLTQSVKNNTQVLINCRNNKKLLGRVKAFD
RHCNMVLENVKEMWTEVPKSGKGKKKSKPVNKDRYISKMFLRGDSVIVVLRNPLIAGK

Sequence of entity 10 (f, m), FASTA

>7DVQ_10 Small nuclear ribonucleoprotein F (chains f, m)
MSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLG
EVLIRCNNVLYIRGVEEEEEDGEMRE

Sequence of entity 11 (e, l), FASTA

>7DVQ_11 Small nuclear ribonucleoprotein E (chains e, l)
MAYRGQGQKVQKVMVQPINLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDD
AEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Sequence of entity 12 (g, n), FASTA

>7DVQ_12 Small nuclear ribonucleoprotein G (chains g, n)
MSKAHPPELKKFMDKKLSLKLNGGRHVQGILRGFDPFMNLVIDECVEMATSGQQNNIGMV
VIRGNSIIMLEALERV

Ligands and cofactors

ID	Name	Formula	Copies
IHP	Inositol hexakisphosphate	C6 H18 O24 P6	1
GTP	Guanosine-5'-triphosphate	C10 H16 N5 O14 P3	1
MG	Magnesium ion	Mg	5
G5J	5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]o…	C11 H18 N5 O14 P3	1
ZN	Zinc ion	Zn	7

Water and common crystallization additives (K) are not listed.

Primary citation

Structure of the activated human minor spliceosome. Bai, R., Wan, R., Wang, L. et al. Science (2021) 371. DOI 10.1126/science.abg0879 · PubMed

Other PDB entries of the same protein (UniProt Q6P2Q9 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4JK8 1.15 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
4JKB 1.3 Å, Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
4JKA 1.32 Å, Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
4JK7 1.4 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
4JK9 1.5 Å, Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
4JKC 1.5 Å, Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
4JKD 1.55 Å, Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
4JKE 1.65 Å, Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
4JKG 1.8 Å, Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain…
4JKH 1.8 Å, Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
3ENB 1.85 Å, Crystal Structure of PRP8 core domain IV
3LRU 1.85 Å, hPRP8 Non-Native Subdomain

Browse structure collections

About this viewer

MolViewer shows 7DVQ directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.