7E9H: Gi-bound metabotropic glutamate receptor mGlu4

Cryo-EM structure of Gi-bound metabotropic glutamate receptor mGlu4. Determined by electron microscopy at 4.0 Å resolution. Released 23 Jun 2021.

Method: Electron microscopy
Resolution: 4.0 Å
Organism: Homo sapiens
Chains: 6
Atoms: 16,847
Mol. weight: 313.85 kDa
Ligands: SEP
Released: 23 Jun 2021

Explore 7E9H in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7E9H contains 68 α-helices and 115 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 6 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	7-30	24
β-strand	36-40	5	17
β-strand	185-188	4	17
β-strand	197-200	4	17
β-strand	220-225	6	17
α-helix	233-235	3
α-helix	242-254	13
β-strand	263-268	6	17
α-helix	271-280	10
α-helix	297-305	9
β-strand	319-323	5	17
α-helix	332-349	18

Chain B: 3 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	38-39	2
β-strand	47-51	5	18
β-strand	61-63	3	19
α-helix	64	1
β-strand	69-72	4	19
β-strand	78	1	20
β-strand	80-83	4	19
β-strand	94	1	20
β-strand	103-105	3	21
β-strand	111-114	4	21
β-strand	116	1	22
β-strand	121-122	2	23
β-strand	123-125	3	21
β-strand	138-139	2	23
α-helix	145	1
β-strand	146	1	22
β-strand	148-151	4	24
β-strand	156-160	5	24
β-strand	168-170	3	24
β-strand	187-192	6	25
β-strand	198-203	6	25
β-strand	207-212	6	25
β-strand	218-223	6	25
β-strand	229-234	6	26
β-strand	240-245	6	26
β-strand	250-254	5	26
β-strand	260-264	5	26
β-strand	273-277	5	27
β-strand	285-289	5	27
β-strand	294-297	4	27
β-strand	304-307	4	27
β-strand	315-320	6	18
β-strand	327-331	5	18
β-strand	336-339	4	18

Chain D: 2 helices, 27 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	28
β-strand	10-12	3	29
β-strand	19	1	30
β-strand	21-25	5	28
α-helix	29-31	3
β-strand	33-35	3	31
β-strand	38-39	2	32
β-strand	45-46	2	32
β-strand	49-51	3	31
β-strand	58-60	3	31
β-strand	65	1	33
β-strand	68	1	33
β-strand	69-70	2	30
β-strand	78	1	28
β-strand	81-82	2	30
β-strand	92	1	29
β-strand	93-94	2	32
β-strand	97-99	3	31
β-strand	117-119	3	29
β-strand	146-147	2	34
β-strand	155-159	5	35
β-strand	175-179	5	34
β-strand	187-190	4	34
β-strand	194-195	2	34
β-strand	203-207	5	35
β-strand	212-216	5	35
α-helix	225	1
β-strand	226-229	4	34
β-strand	244-245	2	34

Chain R: 26 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	43-45	3	1
β-strand	49	1	2
β-strand	50-55	6	1
β-strand	58-60	3	3
β-strand	67-70	4	3
α-helix	75-91	17
β-strand	101	1	2
β-strand	103-107	5	1
α-helix	112-126	15
β-strand	150-154	5	1
α-helix	155-156	2
α-helix	159-169	11
β-strand	176-178	3	1
α-helix	184-187	4
β-strand	195-197	3	1
α-helix	202-215	14
β-strand	220-226	7	4
α-helix	231-245	15
β-strand	249-256	8	4
α-helix	264-274	11
β-strand	281-283	3	4
α-helix	287-299	13
β-strand	308-310	3	4
β-strand	331-334	4	4
α-helix	342-349	8
α-helix	360-369	10
α-helix	404-427	24
α-helix	436-438	3
α-helix	443-451	9
β-strand	455-456	2	5
α-helix	458-460	3
β-strand	462-463	2	5
β-strand	475-481	7	4
β-strand	488-495	8	4
β-strand	499	1	4
α-helix	514-517	4
β-strand	530-532	3	6
β-strand	541-543	3	6
β-strand	550	1	7
β-strand	558	1	7
β-strand	564-566	3	8
α-helix	567	1
β-strand	573-575	3	8
α-helix	576-577	2
β-strand	578	1	9
α-helix	589-611	23
α-helix	616-620	5
α-helix	623-643	21
α-helix	649-686	38
α-helix	696-720	25
β-strand	742	1	9
α-helix	749-774	26
α-helix	785-810	26
α-helix	815-847	33

Chain S: 31 helices, 23 β-strands

Element	Residues	Length	Sheet
β-strand	43-45	3	10
β-strand	49-50	2	11
β-strand	52-55	4	10
α-helix	67-70	4
α-helix	73-77	5
α-helix	78-91	14
β-strand	101-102	2	11
β-strand	103-107	5	10
α-helix	112-122	11
β-strand	153-154	2	10
α-helix	159-171	13
β-strand	176-177	2	10
α-helix	184-186	3
β-strand	195	1	10
α-helix	202-215	14
β-strand	220-225	6	12
α-helix	228-241	14
α-helix	242-246	5
β-strand	249-255	7	12
α-helix	266-273	8
β-strand	280-283	4	12
α-helix	287-298	12
β-strand	307-309	3	12
α-helix	312-314	3
α-helix	321-326	6
β-strand	331-336	6	12
α-helix	342-348	7
α-helix	361-369	9
α-helix	406-427	22
α-helix	436-438	3
α-helix	443-450	8
β-strand	456	1	13
β-strand	462	1	13
β-strand	475-481	7	12
β-strand	488-491	4	12
β-strand	530-533	4	14
β-strand	540-543	4	14
β-strand	550	1	15
β-strand	558	1	15
α-helix	560-561	2
β-strand	564-565	2	16
β-strand	574-575	2	16
α-helix	576-579	4
α-helix	586-588	3
α-helix	589-611	23
α-helix	616-618	3
α-helix	624-643	20
α-helix	649-681	33
α-helix	697-719	23
α-helix	750-773	24
α-helix	784-799	16
α-helix	801-807	7
α-helix	814-835	22
α-helix	839-847	9

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Metabotropic glutamate receptor 4	R, S	protein	890	Homo sapiens	Q14833 (AlphaFold model)
Guanine nucleotide-binding protein G(i) subunit alpha-3	A	protein	354	Homo sapiens	P08754 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv16	D	protein	257	Homo sapiens

Sequence of entity 1 (R, S), FASTA

>7E9H_1 Metabotropic glutamate receptor 4 (chains R, S)
DYKDDDDGAPKPKGHPHMNSIRIDGDITLGGLFPVHGRGSEGKPCGELKKEKGIHRLEAM
LFALDRINNDPDLLPNITLGARILDTCSRDTHALEQSLTFVQALIEKDGTEVRCGSGGPP
IITKPERVVGVIGASGSSVSIMVANILRLFKIPQISYASTAPDLSDNSRYDFFSRVVPSD
TYQAQAMVDIVRALKWNYVSTVASEGSYGESGVEAFIQKSREDGGVCIAQSVKIPREPKA
GEFDKIIRRLLETSNARAVIIFANEDDIRRVLEAARRANQTGHFFWMGSDSWGSKIAPVL
HLEEVAEGAVTILPKRMSVRGFDRYFSSRTLDNNRRNIWFAEFWEDNFHCKLSRHALKKG
SHVKKCTNRERIGQDSAYEQEGKVQFVIDAVYAMGHALHAMHRDLCPGRVGLCPRMDPVD
GTQLLKYIRNVNFSGIAGNPVTFNENGDAPGRYDIYQYQLRNDSAEYKVIGSWTDHLHLR
IERMHWPGSGQQLPRSICSLPCQPGERKKTVKGMPCCWHCEPCTGYQYQVDRYTCKTCPY
DMRPTENRTGCRPIPIIKLEWGSPWAVLPLFLAVVGIAATLFVVITFVRYNDTPIVKASG
RELSYVLLAGIFLCYATTFLMIAEPDLGTCSLRRIFLGLGMSISYAALLTKTNRIYRIFE
QGKRSVSAPRFISPASQLAITFSLISLQLLGICVWFVVDPSHSVVDFQDQRTLDPRFARG
VLKCDISDLSLICLLGYSMLLMVTCTVYAIKTRGVPETFNEAKPIGFTMYTTCIVWLAFI
PIFFGTSQSADKLYIQTTTLTVSVSLSASVSLGMLYMPKVYIILFHPEQNVPKRKRSLKA
VVTAATMSNKFTQKGNFRPNGEAKSELCENLEAPALATKQTYVTYTNHAI

Sequence of entity 2 (A), FASTA

>7E9H_2 Guanine nucleotide-binding protein G(i) subunit alpha-3 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKNTIVKQMKIIHEDG
YSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAARADDARQLFVLAGSAEEGVMT
PELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQSNYIPTQQDVLRTRVK
TTGIVETHFTDKDLYFKMFDVGAQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHASMKLFDSICNNKWFTETSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAA
AYIQCQFEDLNRRKDTKEIYTHFTCSTDTKNVQFVFDAVTDVIIKNNLKECGLY

Sequence of entity 3 (B), FASTA

>7E9H_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (C), FASTA

>7E9H_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 5 (D), FASTA

>7E9H_5 scFv16 (chains D)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAALEVLFQ

Ligands and cofactors

ID	Name	Formula	Copies
SEP	Phosphoserine	C3 H8 N O6 P	2

Primary citation

Structures of G i -bound metabotropic glutamate receptors mGlu2 and mGlu4. Lin, S., Han, S., Cai, X. et al. Nature (2021) 594:583-588. DOI 10.1038/s41586-021-03495-2 · PubMed

Other PDB entries of the same protein (UniProt Q14833 (AlphaFold model), which also has an AlphaFold model), best resolution first:

8JD4 2.9 Å, Cryo-EM structure of G protein-free mGlu2-mGlu4 heterodimer in Acc state
8JD6 3.4 Å, Cryo-EM structure of Gi1-bound metabotropic glutamate receptor mGlu4
8JD5 3.6 Å, Cryo-EM structure of Gi1-bound mGlu2-mGlu4 heterodimer
8WGB 3.7 Å, mGlu2-4 heterodimer bound with Gi
8WGD 4.45 Å, mGlu2-4 inactive heterodimer
8WG9 4.46 Å, mGlu2-mGlu4 heterodimer bound mGlu4 agonist E7P
8WGC 5.95 Å, heterodimer of mGlu2 and mGlu4 bound with mGlu2 agonist LY379268

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