7EGG: TFIID lobe B subcomplex
TFIID lobe B subcomplex. Determined by electron microscopy at 2.77 Å resolution. Released 5 May 2021.
- Method
- Electron microscopy
- Resolution
- 2.77 Å
- Organism
- Homo sapiens
- Chains
- 7
- Atoms
- 10,062
- Mol. weight
- 372.89 kDa
- Released
- 5 May 2021
Explore 7EGG in 3D
Show helices and sheets
RCSB PDB
PDBe
Secondary structure: helices and β-sheets
7EGG contains 64 α-helices and 47 β-strands across 7 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.
Chain D: 7 helices, 2 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 843-856 | 14 | |
| α-helix | 875-885 | 11 | |
| α-helix | 886-888 | 3 | |
| α-helix | 899-921 | 23 | |
| β-strand | 934-936 | 3 | 1 |
| α-helix | 940-969 | 30 | |
| β-strand | 1056-1058 | 3 | 2 |
| α-helix | 1060-1065 | 6 | |
| α-helix | 1077-1082 | 6 | |
Chain E: 28 helices, 33 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 211-213 | 3 | |
| α-helix | 214-226 | 13 | |
| α-helix | 230-236 | 7 | |
| α-helix | 237-239 | 3 | |
| α-helix | 240-253 | 14 | |
| α-helix | 257-267 | 11 | |
| α-helix | 268-270 | 3 | |
| α-helix | 273-275 | 3 | |
| α-helix | 276-284 | 9 | |
| α-helix | 288-291 | 4 | |
| α-helix | 297-302 | 6 | |
| β-strand | 305 | 1 | 3 |
| β-strand | 308-310 | 3 | 4 |
| α-helix | 311-318 | 8 | |
| α-helix | 324-326 | 3 | |
| α-helix | 329-336 | 8 | |
| β-strand | 337 | 1 | 3 |
| β-strand | 340-342 | 3 | 4 |
| α-helix | 345-348 | 4 | |
| α-helix | 349-355 | 7 | |
| α-helix | 376-377 | 2 | |
| α-helix | 422-423 | 2 | |
| α-helix | 429-431 | 3 | |
| α-helix | 434-448 | 15 | |
| α-helix | 450-452 | 3 | |
| α-helix | 458-460 | 3 | |
| β-strand | 461-466 | 6 | 5 |
| α-helix | 472 | 1 | |
| β-strand | 473-478 | 6 | 6 |
| β-strand | 484-489 | 6 | 6 |
| β-strand | 493-498 | 6 | 6 |
| β-strand | 504-507 | 4 | 7 |
| α-helix | 508-509 | 2 | |
| α-helix | 510-515 | 6 | |
| α-helix | 524-527 | 4 | |
| β-strand | 528-534 | 7 | 7 |
| β-strand | 536-540 | 5 | 6 |
| β-strand | 546-550 | 5 | 8 |
| β-strand | 558-562 | 5 | 8 |
| β-strand | 566-570 | 5 | 8 |
| β-strand | 577-582 | 6 | 8 |
| β-strand | 588-593 | 6 | 9 |
| β-strand | 600-604 | 5 | 9 |
| β-strand | 609-612 | 4 | 9 |
| β-strand | 621-623 | 3 | 9 |
| β-strand | 634-635 | 2 | 10 |
| β-strand | 641-645 | 5 | 10 |
| β-strand | 650-655 | 6 | 10 |
| β-strand | 661-666 | 6 | 10 |
| β-strand | 672-677 | 6 | 11 |
| β-strand | 683-688 | 6 | 11 |
| β-strand | 693-697 | 5 | 11 |
| β-strand | 703-707 | 5 | 11 |
| β-strand | 714-719 | 6 | 12 |
| β-strand | 725-730 | 6 | 12 |
| β-strand | 734-739 | 6 | 12 |
| α-helix | 742-744 | 3 | |
| α-helix | 765-768 | 4 | |
| β-strand | 769-774 | 6 | 12 |
| β-strand | 782-785 | 4 | 5 |
| β-strand | 791-796 | 6 | 5 |
Chain F: 6 helices, 6 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 16-24 | 9 | |
| α-helix | 33-60 | 28 | |
| β-strand | 66 | 1 | 13 |
| α-helix | 68-77 | 10 | |
| β-strand | 86-88 | 3 | 14 |
| α-helix | 116-119 | 4 | |
| α-helix | 121-124 | 4 | |
| β-strand | 125 | 1 | 15 |
| α-helix | 127-129 | 3 | |
| β-strand | 131-137 | 7 | 5 |
| β-strand | 138 | 1 | 16 |
| β-strand | 141 | 1 | 16 |
Chain H: 8 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 25-48 | 24 | |
| α-helix | 55-82 | 28 | |
| α-helix | 98-100 | 3 | |
| α-helix | 104-106 | 3 | |
| α-helix | 107-111 | 5 | |
| α-helix | 119-123 | 5 | |
| β-strand | 124 | 1 | 15 |
| α-helix | 140-143 | 4 | |
| α-helix | 151-153 | 3 | |
Chain I: 7 helices, 3 β-strands
| Element | Residues | Length | Sheet |
|---|
| α-helix | 14-25 | 12 | |
| β-strand | 31 | 1 | 13 |
| α-helix | 33-61 | 29 | |
| α-helix | 68-76 | 9 | |
| α-helix | 86-88 | 3 | |
| α-helix | 89-99 | 11 | |
| α-helix | 103-106 | 4 | |
| β-strand | 121 | 1 | 8 |
| β-strand | 127-129 | 3 | 1 |
| α-helix | 130-131 | 2 | |
Chain J: 4 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 115-122 | 8 | |
| α-helix | 132-140 | 9 | |
| α-helix | 149-176 | 28 | |
| α-helix | 197-203 | 7 | |
| β-strand | 209-211 | 3 | 14 |
Chain L: 4 helices, 1 β-strand
| Element | Residues | Length | Sheet |
|---|
| α-helix | 62-70 | 9 | |
| β-strand | 74-76 | 3 | 2 |
| α-helix | 78-105 | 28 | |
| α-helix | 113-119 | 7 | |
| α-helix | 120-124 | 5 | |
Molecules and chains
| Molecule | Chains | Type | Length | Organism | UniProt |
|---|
| Transcription initiation factor TFIID subunit 4 | D | protein | 1085 | Homo sapiens | O00268 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 5 | E | protein | 800 | Homo sapiens | Q15542 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 6 | F | protein | 677 | Homo sapiens | P49848 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 8 | H | protein | 310 | Homo sapiens | Q7Z7C8 (AlphaFold model) |
| Transcription initiation factor TFIID subunit 9 | I | protein | 264 | Homo sapiens | Q16594 |
| Transcription initiation factor TFIID subunit 10 | J | protein | 218 | Homo sapiens | Q12962 |
| Transcription initiation factor TFIID subunit 12 | L | protein | 161 | Homo sapiens | Q16514 |
Sequence of entity 1 (D), FASTA
>7EGG_1 Transcription initiation factor TFIID subunit 4 (chains D)
MAAGSDLLDEVFFNSEVDEKVVSDLVGSLESQLAASAAHHHHLAPRTPEVRAAAAGALGN
HVVSGSPAGAAGAGPAAPAEGAPGAAPEPPPAGRARPGGGGPQRPGPPSPRRPLVPAGPA
PPAAKLRPPPEGSAGSCAPVPAAAAVAAGPEPAPAGPAKPAGPAALAARAGPGPGPGPGP
GPGPGPGKPAGPGAAQTLNGSAALLNSHHAAAPAVSLVNNGPAALLPLPKPAAPGTVIQT
PPFVGAAAPPAPAAPSPPAAPAPAAPAAAPPPPPPAPATLARPPGHPAGPPTAAPAVPPP
AAAQNGGSAGAAPAPAPAAGGPAGVSGQPGPGAAAAAPAPGVKAESPKRVVQAAPPAAQT
LAASGPASTAASMVIGPTMQGALPSPAAVPPPAPGTPTGLPKGAAGAVTQSLSRTPTATT
SGIRATLTPTVLAPRLPQPPQNPTNIQNFQLPPGMVLVRSENGQLLMIPQQALAQMQAQA
HAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATL
QRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCKNFL
STLIKLASSGKQSTETAANVKELVQNLLDGKIEAEDFTSRLYRELNSSPQPYLVPFLKRS
LPALRQLTPDSAAFIQQSQQQPPPPTSQATTALTAVVLSSSVQRTAGKTAATVTSALQPP
VLSLTQPTQVGVGKQGQPTPLVIQQPPKPGALIRPPQVTLTQTPMVALRQPHNRIMLTTP
QQIQLNPLQPVPVVKPAVLPGTKALSAVSAQAAAAQKNKLKEPGGGSFRDDDDINDVASM
AGVNLSEESARILATNSELVGTLTRSCKDETFLLQAPLQRRILEIGKKHGITELHPDVVS
YVSHATQQRLQNLVEKISETAQQKNFSYKDDDRYEQASDVRAQLKFFEQLDQIEKQRKDE
QEREILMRAAKSRSRQEDPEQLRLKQKAKEMQQQELAQMRQRDANLTALAAIGPRKKRKV
DCPGPGSGAEGSGPGSVVPGSSGVGTPRQFTRQRITRVNLRDLIFCLENERETSHSLLLY
KAFLK
Sequence of entity 2 (E), FASTA
>7EGG_2 Transcription initiation factor TFIID subunit 5 (chains E)
MAALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGPNGGGGNVAASSSTGGD
GGTPKPTVAVSAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQFLRQSKLREAEEALRREA
GLLEEAVAGSGAPGEVDSAGAEVTSALLSRVTASAPGPAAPDPPGTGASGATVVSGSASG
PAAPGKVGSVAVEDQPDVSAVLSAYNQQGDPTMYEEYYSGLKHFIECSLDCHRAELSQLF
YPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQDDLRVLSSLTKKEHMKGNETMLDF
RTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQEHLYIDIFDGMPRSKQQIDAMVGSLA
GEAKREANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKPKKKKPKKDSIGSKSKKQDPN
APPQNRIPLPELKDSDKLDKIMNMKETTKRVRLGPDCLPSICFYTFLNAYQGLTAVDVTD
DSSLIAGGFADSTVRVWSVTPKKLRSVKQASDLSLIDKESDDVLERIMDEKTASELKILY
GHSGPVYGASFSPDRNYLLSSSEDGTVRLWSLQTFTCLVGYKGHNYPVWDTQFSPYGYYF
VSGGHDRVARLWATDHYQPLRIFAGHLADVNCTRFHPNSNYVATGSADRTVRLWDVLNGN
CVRIFTGHKGPIHSLTFSPNGRFLATGATDGRVLLWDIGHGLMVGELKGHTDTVCSLRFS
RDGEILASGSMDNTVRLWDAIKAFEDLETDDFTTATGHINLPENSQELLLGTYMTKSTPV
VHLHFTRRNLVLAAGAYSPQ
Sequence of entity 3 (F), FASTA
>7EGG_3 Transcription initiation factor TFIID subunit 6 (chains F)
MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHM
GKRQKLTTSDIDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINT
PLPRVPLDVCLKAHWLSIEGCQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKG
KGQGATTADGKGKEKKAPPLLEGAPLRLKPRSIHELSVEQQLYYKEITEACVGSCEAKRA
EALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLIYLMRMVKALMDNPTLYLEKY
VHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNNIQSRITKTFT
KSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGA
DHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRT
TLTITQPRPTLTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIV
MSSSSSAPSTQQVLSLSTSAPGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPSGP
GSVQKYIVVSLPPTGEGKGGPTSHPSPVPPPASSPSPLSGSALCGGKQEAGDSPPPAPGT
PKANGSQPNSGSPQPAP
Sequence of entity 4 (H), FASTA
>7EGG_4 Transcription initiation factor TFIID subunit 8 (chains H)
MADAAATAGAGGSGTRSGSKQSTNPADNYHLARRRTLQVVVSSLLTEAGFESAEKASVET
LTEMLQSYISEIGRSAKSYCEHTARTQPTLSDIVVTLVEMGFNVDTLPAYAKRSQRMVIT
APPVTNQPVTPKALTAGQNRPHPPHIPSHFPEFPDPHTYIKTPTYREPVSDYQVLREKAA
SQRRDVERALTRFMAKTGETQSLFKDDVSTFPLIAARPFTIPYLTALLPSELEMQQMEET
DSSEQDEQTDTENLALHISMEDSGAEKENTSVLQQNPSLSGSRNGEENIIDNPYLRPVKK
PKIRRKKSLS
Sequence of entity 5 (I), FASTA
>7EGG_5 Transcription initiation factor TFIID subunit 9 (chains I)
MESGKTASPKSMPKDAQMMAQILKDMGITEYEPRVINQMLEFAFRYVTTILDDAKIYSSH
AKKATVDADDVRLAIQCRADQSFTSPPPRDFLLDIARQRNQTPLPLIKPYSGPRLPPDRY
CLTAPNYRLKSLQKKASTSAGRITVPRLSVGSVTSRPSTPTLGTPTPQTMSVSTKVGTPM
SLTGQRFTVQMPTSQSPAVKASIPATSAVQNVLINPSLIGSKNILITTNMMSSQNTANES
SNALKRKREDDDDDDDDDDDYDNL
Sequence of entity 6 (J), FASTA
>7EGG_6 Transcription initiation factor TFIID subunit 10 (chains J)
MSCSGSGADPEAAPASAASAPGPAPPVSAPAALPSSTAAENKASPAGTAGGPGAGAAAGG
TGPLAARAGEPAERRGAAPVSAGGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFL
MQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTA
SGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT
Sequence of entity 7 (L), FASTA
>7EGG_7 Transcription initiation factor TFIID subunit 12 (chains L)
MNQFGPSALINLSNFSSIKPEPASTPPQGSMANSTAVVKIPGTPGAGGRLSPENNQVLTK
KKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLEVKDVQLHL
ERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKTTKK
Primary citation
Structural insights into preinitiation complex assembly on core promoters. Chen, X., Qi, Y., Wu, Z. et al. Science (2021) 372. DOI 10.1126/science.aba8490 · PubMed
Other PDB entries of the same protein (UniProt O00268 (AlphaFold model), which also has an AlphaFold model), best resolution first:
- 2P6V 2.0 Å, Structure of TAFH domain of the human TAF4 subunit of TFIID
- 1H3O 2.3 Å, Crystal Structure of the Human TAF4-TAF12 (TAFII135-TAFII20) Complex
- 7EGF 3.16 Å, TFIID lobe A subcomplex
- 7EGB 3.3 Å, TFIID-based holo PIC on SCP promoter
- 7EG9 3.7 Å, TFIID-based intermediate PIC on SCP promoter
- 7EGC 3.9 Å, p53-bound TFIID-based holo PIC on HDM2 promoter
- 7ENA 4.07 Å, TFIID-based PIC-Mediator holo-complex in pre-assembled state (pre-hPIC-MED)
- 7EGA 4.1 Å, TFIID-based intermediate PIC on PUMA promoter
- 7ENC 4.13 Å, TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)
- 8GXS 4.16 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
- 6MZC 4.5 Å, Human TFIID BC core
- 7EDX 4.5 Å, p53-bound TFIID-based core PIC on HDM2 promoter
Browse structure collections
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