7F6G: Angiotensin receptor AT1R

Cryo-EM structure of human angiotensin receptor AT1R in complex Gq proteins and Sar1-AngII. Determined by electron microscopy at 2.9 Å resolution. Released 29 Mar 2023.

Method: Electron microscopy
Resolution: 2.9 Å
Organism: Homo sapiens
Chains: 5
Atoms: 8,697
Mol. weight: 176.98 kDa
Ligands: NAG, CLR
Released: 29 Mar 2023

Explore 7F6G in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F6G contains 41 α-helices and 41 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 15 helices, 4 β-strands

Element	Residues	Length	Sheet
β-strand	12-14	3	1
α-helix	27-52	26
α-helix	53-57	5
α-helix	62-79	18
α-helix	81-89	9
α-helix	98-131	34
α-helix	136-139	4
α-helix	142-166	25
β-strand	168-172	5	1
β-strand	177-181	5	1
β-strand	182-184	3	2
α-helix	190-200	11
α-helix	201-205	5
α-helix	206-229	24
α-helix	235-268	34
α-helix	274-291	18
α-helix	293-299	7
α-helix	300-304	5
α-helix	307-319	13

Chain B: 18 helices, 8 β-strands

Element	Residues	Length	Sheet
α-helix	13-35	23
α-helix	36-38	3
β-strand	39-46	8	3
α-helix	52-62	11
α-helix	69-96	28
α-helix	104-115	12
α-helix	126-137	12
α-helix	139-146	8
α-helix	157-162	6
α-helix	164-168	5
α-helix	176-181	6
β-strand	190-195	6	3
β-strand	200-205	6	3
α-helix	213-216	4
α-helix	217-220	4
β-strand	225-231	7	3
α-helix	232-234	3
β-strand	238-239	2	4
β-strand	245-246	2	4
α-helix	247-260	14
α-helix	262-266	5
β-strand	268-274	7	3
α-helix	276-282	7
α-helix	302-315	14
β-strand	324-328	5	3
α-helix	336-355	20

Chain C: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	34-36	3
β-strand	47-51	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	88-94	7	6
α-helix	95	1
β-strand	100-105	6	7
β-strand	111-116	6	7
β-strand	121-125	5	7
β-strand	134-139	6	7
β-strand	146-151	6	8
β-strand	157-161	5	8
β-strand	166-169	4	8
β-strand	176-180	5	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	208-212	5	9
β-strand	218-222	5	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	250-254	5	10
β-strand	259-264	6	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	294-298	5	11
β-strand	304-308	5	11
β-strand	315-320	6	5
β-strand	327-331	5	5
β-strand	336-339	4	5

Chain D: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-23	15
α-helix	30-43	14
α-helix	53-55	3
α-helix	56-58	3

Chain L: 0 helices, 1 β-strand

Element	Residues	Length	Sheet
β-strand	2-4	3	2

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Angiotensin receptor AT1R	A	protein	725	Homo sapiens
SAR1-AngII	L	protein	8	Homo sapiens
Guanine nucleotide-binding protein G(q) subunit alpha	B	protein	369	Homo sapiens	P50148 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	354	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	81	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7F6G_1 Angiotensin receptor AT1R (chains A)
MDYKDDDDKHHHHHHHHHHENLYFQGKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIK
VTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTW
DAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEP
YFTWPLIAADGGYAFKYAAGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAE
AAFNKGETAMTINGPWAWSNIDTSAVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKE
LAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIP
QMSAFWYAVRTAVINAASGRQTVDEALKDAQTILNSSTEDGIKRIQDDCPKAGRHNYIFV
MIPTLYSIIFVVGIFGNSLVVIVIYFYMKLKTVASVFLLNLALADLCALLTLPLWAVYTA
MEYRWPFGNYLCKIASASVSFNLYASVFLLTCLSIDRYLAIVHPMKSRLRRTMLVAKVTC
IIIWLLAGLASLPAIIHRNVFFIENTNITVCAFHYESQNSTLPIGLGLTKNILGFLFPFL
IILTSYTLIWKALKKAYEIQKNKPRNDDIFKIIMAIVLFFFFSWIPHQIFTFLDVLIQLG
IIRDCRIADIVDTAMPITICIAYFNACLNPLFYGFLGKKFKRYFLQLLKYIPPKAKSHSN
LSTKM

Sequence of entity 2 (L), FASTA

>7F6G_2 SAR1-AngII (chains L)
GRVYIHPF

Sequence of entity 3 (B), FASTA

>7F6G_3 Guanine nucleotide-binding protein G(q) subunit alpha (chains B)
MHHHHHHHHHHTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGES
GKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHA
QLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADP
AYLPTQQDVLRVQVPTTGIIEYPFDLQSVIFRMVDVGGLRSERRKWIHCFENVTSIMFLV
ALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVD
YFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQ
LNLKEYNLV

Sequence of entity 4 (C), FASTA

>7F6G_4 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MHHHHHHHHHHGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMR
TRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYA
PSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTC
ALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHE
SDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLL
AGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 5 (D), FASTA

>7F6G_5 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MHHHHHHHHHHASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPL
LTPVPASENPFREKKFFCAIL

Ligands and cofactors

ID	Name	Formula	Copies
NAG	2-acetamido-2-deoxy-beta-D-glucopyranose	C8 H15 N O6	1
CLR	Cholesterol	C27 H46 O	6

Primary citation

Structural insights into angiotensin receptor signaling modulation by balanced and biased agonists. Zhang, D., Liu, Y., Zaidi, S.A. et al. EMBO J (2023) 42:e112940-e112940. DOI 10.15252/embj.2022112940 · PubMed

Other PDB entries of the same protein (UniProt P50148 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9VAW 2.24 Å, Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gq (delipidated) complex in…
9M1H 2.55 Å, Cryo-EM structure of PGE2-EP1-Gq complex
9XIX 2.58 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the C-state
8THK 2.6 Å, Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with…
9XH2 2.6 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state
8G59 2.64 Å, Cryo-EM structure of the TUG891 bound GPR120-Giq complex
24XB 2.65 Å, Cryo-EM Structure of the Apo FFAR4-Gq Complex
8XGO 2.68 Å, a peptide receptor complex structure
9K27 2.68 Å, PrRP31 bound prolactin-releasing peptide receptor coupled with Gq protein complex
9XJP 2.74 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state, chemokine domain fully…
9LL9 2.76 Å, DOI-bound Serotonin 2A (5-HT2A) receptor-Gq complex
7F6I 2.8 Å, Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and kallidin

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