7F8W: Cholecystokinin receptor CCKBR

Cryo-EM structure of the cholecystokinin receptor CCKBR in complex with gastrin-17 and Gq. Determined by electron microscopy at 3.1 Å resolution. Released 13 Oct 2021.

Method: Electron microscopy
Resolution: 3.1 Å
Organism: Homo sapiens
Chains: 6
Atoms: 8,486
Mol. weight: 169.2 kDa
Released: 13 Oct 2021

Explore 7F8W in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7F8W contains 27 α-helices and 63 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-29	21
β-strand	36-39	4	1
α-helix	46-56	11
β-strand	184-187	4	1
β-strand	196-199	4	1
α-helix	207-211	5
β-strand	220-223	4	1
α-helix	231-233	3
α-helix	243-253	11
β-strand	265-266	2	1
α-helix	274-279	6
α-helix	298-309	12
β-strand	321	1	1
α-helix	330-349	20

Chain B: 2 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	38-39	2
β-strand	47-52	6	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	103-105	3	4
β-strand	111-114	4	4
β-strand	121-125	5	4
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	166-170	5	5
β-strand	176-180	5	5
β-strand	187-191	5	6
β-strand	198-203	6	6
β-strand	208-212	5	6
β-strand	218-222	5	6
β-strand	231-234	4	7
β-strand	240-244	5	7
β-strand	249	1	8
β-strand	252-254	3	7
β-strand	265	1	8
α-helix	272	1
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	306-307	2	9
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	335-339	5	2

Chain C: 2 helices, 0 β-strands

Element	Residues	Length	Sheet
α-helix	30-43	14
α-helix	53-55	3

Chain D: 2 helices, 26 β-strands

Element	Residues	Length	Sheet
β-strand	21-24	4	10
β-strand	28-29	2	11
β-strand	35-41	7	10
α-helix	46-48	3
β-strand	50-52	3	12
β-strand	55-56	2	13
β-strand	62-63	2	13
β-strand	66-68	3	12
β-strand	76-77	2	12
α-helix	79-81	3
β-strand	86-90	5	10
β-strand	95-100	6	10
β-strand	109-111	3	13
β-strand	114-116	3	12
β-strand	127-128	2	12
β-strand	132-134	3	13
β-strand	135-136	2	11
β-strand	158-159	2	14
β-strand	163-165	3	15
β-strand	172-177	6	14
β-strand	191-196	6	16
β-strand	202-207	6	16
β-strand	211-212	2	16
β-strand	220-224	5	14
β-strand	229-233	5	14
β-strand	243-248	6	16
β-strand	260	1	16
β-strand	261-263	3	15

Chain R: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	56-80	25
α-helix	88-116	29
α-helix	124-156	33
α-helix	159-165	7
α-helix	168-185	18
β-strand	197	1	17
β-strand	203	1	17
α-helix	212-223	12
α-helix	224-228	5
α-helix	229-248	20
α-helix	327-358	32
α-helix	363-366	4
α-helix	370-387	18
α-helix	388-392	5
α-helix	395-404	10

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(q) subunit alpha	A	protein	353	Homo sapiens	P50148 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	351	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv16	D	protein	268	Homo sapiens
Gastrin-17	E	protein	17	Homo sapiens	P01350 (AlphaFold model)
Gastrin/cholecystokinin type B receptor	R	protein	465	Homo sapiens	P32239

Sequence of entity 1 (A), FASTA

>7F8W_1 Guanine nucleotide-binding protein G(q) subunit alpha (chains A)
MGCTLSAEDKAAVERSKMIDRNLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSG
YSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFEN
PYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPAYLPTQQDVLRVRVPT
TGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNEN
RMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAR
EFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV

Sequence of entity 2 (B), FASTA

>7F8W_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MHHHHHHGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRR
TLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSG
NYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALW
DIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDI
NAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGY
DDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>7F8W_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (D), FASTA

>7F8W_4 scFv16 (chains D)
MVRTAVLILLLVRFSEPDVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEK
GLEWVAYISSGSGTIYYADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYY
GSSPFDFWGQGTTLTVSSGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSK
SLLHSNGNTYLYWFLQRPGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAED
VGVYYCMQHLEYPLTFGAGTKLELKAAA

Sequence of entity 5 (E), FASTA

>7F8W_5 Gastrin-17 (chains E)
QGPWLEEEEEAYGWMDF

Sequence of entity 6 (R), FASTA

>7F8W_6 Gastrin/cholecystokinin type B receptor (chains R)
DYKDDDDGAPELLKLNRSVQGTGPGPGASLCRPGAPLLNSSSVGNLSCEPPRIRGAGTRE
LELAIRITLYAVIFLMSVGGNMLIIVVLGLSRRLRTVTNAFLLSLAVSDLLLAVACMPFT
LLPNLMGTFIFGTVICKAVSYLMGVSVSVSTLSLVAIALERYSAICRPLQARVWQTRSHA
ARVIVATWLLSGLLMVPYPVYTVVQPVGPRVLQCVHRWPSARVRQTWSVLLLLLLFFIPG
VVMAVAYGLISRELYLGLRFDGDSDSDSQSRVRNQGGLPGAVHQNGRCRPETGAVGEDSD
GCYVQLPRSRPALELTALTAPGPGSGSRPTQAKLLAKKRVVRMLLVIVVLFFLCWLPVYS
ANTWRAFDGPGAHRALSGAPISFIHLLSYASACVNPLVYCFMHRRFRQACLETCARCCPR
PPRARPREFLEVLFQGPWSHPQFEKGGGSGGGSGGSAWSHPQFEK

Primary citation

Structures of the human cholecystokinin receptors bound to agonists and antagonists. Zhang, X., He, C., Wang, M. et al. Nat Chem Biol (2021) 17:1230-1237. DOI 10.1038/s41589-021-00866-8 · PubMed

Other PDB entries of the same protein (UniProt P50148 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9VAW 2.24 Å, Cryo-EM structure of human Neurotensin Receptor 1 (hNTSR1)-Gq (delipidated) complex in…
9M1H 2.55 Å, Cryo-EM structure of PGE2-EP1-Gq complex
9XIX 2.58 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the C-state
8THK 2.6 Å, Cryo-EM structure of A61603-bound alpha-1A-adrenergic receptor in complex with…
9XH2 2.6 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state
8G59 2.64 Å, Cryo-EM structure of the TUG891 bound GPR120-Giq complex
24XB 2.65 Å, Cryo-EM Structure of the Apo FFAR4-Gq Complex
8XGO 2.68 Å, a peptide receptor complex structure
9K27 2.68 Å, PrRP31 bound prolactin-releasing peptide receptor coupled with Gq protein complex
9XJP 2.74 Å, Structure of the CX3CL1.44-US28-GqiN18-scFv16 in the E-state, chemokine domain fully…
9LL9 2.76 Å, DOI-bound Serotonin 2A (5-HT2A) receptor-Gq complex
7F6I 2.8 Å, Cryo-EM structure of human bradykinin receptor BK2R in complex Gq proteins and kallidin

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