7LBM: PDB entry 7LBM

Structure of the human Mediator-bound transcription pre-initiation complex. Determined by electron microscopy at 4.8 Å resolution. Released 24 Mar 2021.

Method: Electron microscopy
Resolution: 4.8 Å
Organism: Homo sapiens
Chains: 56
Atoms: 129,277
Mol. weight: 2509.24 kDa
Ligands: MG, ZN, SF4
Released: 24 Mar 2021

Explore 7LBM in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7LBM contains 700 α-helices and 557 β-strands across 54 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 0: 21 helices, 47 β-strands

Element	Residues	Length	Sheet
β-strand	15-20	6	1
β-strand	37-39	3	2
β-strand	45-47	3	2
β-strand	62-65	4	2
β-strand	74-76	3	2
β-strand	86-89	4	3
β-strand	91	1	4
β-strand	93	1	4
β-strand	95-99	5	3
β-strand	105-110	6	3
β-strand	118-124	7	3
α-helix	133-134	2
β-strand	135-136	2	5
α-helix	137-139	3
β-strand	145	1	6
α-helix	155-158	4
β-strand	159	1	6
α-helix	175-176	2
β-strand	177-178	2	7
β-strand	179-180	2	5
β-strand	181-183	3	8
β-strand	187-189	3	8
β-strand	192-193	2	7
β-strand	199-200	2	7
β-strand	203-205	3	8
β-strand	215-219	5	9
β-strand	228-232	5	9
β-strand	235	1	10
β-strand	242-246	5	9
β-strand	255-259	5	9
β-strand	279-285	7	10
β-strand	293-300	8	10
β-strand	303-313	11	10
β-strand	337-344	8	10
β-strand	348	1	10
α-helix	350	1
β-strand	351-355	5	11
α-helix	365-374	10
β-strand	382-386	5	11
β-strand	391-394	4	11
β-strand	401-403	3	11
β-strand	431-434	4	1
β-strand	442	1	12
β-strand	445-447	3	1
β-strand	453-455	3	1
β-strand	456	1	12
α-helix	467-484	18
α-helix	489-494	6
α-helix	500-512	13
α-helix	518-522	5
α-helix	525-534	10
α-helix	542-560	19
α-helix	576-581	6
α-helix	582-587	6
α-helix	608-613	6
α-helix	615-630	16
α-helix	651-666	16
α-helix	670-672	3
β-strand	678	1	13
α-helix	685-701	17
α-helix	711-717	7
α-helix	720-723	4
β-strand	724	1	13
β-strand	749-751	3	1
β-strand	754	1	1
β-strand	795-797	3	14
β-strand	804-806	3	14
β-strand	835	1	14

Chain 1: 89 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	2-13	12
α-helix	33-49	17
α-helix	56-69	14
α-helix	73-88	16
α-helix	94-101	8
α-helix	112-125	14
α-helix	131-147	17
β-strand	152-153	2	20
α-helix	156-160	5
α-helix	162-171	10
α-helix	180-190	11
α-helix	203-209	7
α-helix	213-216	4
α-helix	224-226	3
α-helix	260-263	4
α-helix	267-275	9
α-helix	280-286	7
α-helix	298-314	17
α-helix	328-341	14
α-helix	342-346	5
α-helix	349-360	12
α-helix	369-383	15
α-helix	390-392	3
α-helix	394-402	9
α-helix	410-412	3
α-helix	419-424	6
α-helix	427-437	11
α-helix	439-441	3
α-helix	446-448	3
α-helix	449-451	3
α-helix	452-463	12
α-helix	473-480	8
α-helix	489-500	12
β-strand	507-508	2	21
β-strand	515-516	2	21
α-helix	521-524	4
α-helix	525-528	4
α-helix	533-553	21
β-strand	558	1	21
α-helix	561-571	11
α-helix	575-578	4
α-helix	580-583	4
α-helix	584-588	5
α-helix	589-594	6
α-helix	598-611	14
α-helix	617-633	17
α-helix	639-655	17
α-helix	661-668	8
α-helix	681-697	17
α-helix	711-722	12
α-helix	729-732	4
α-helix	737-745	9
α-helix	753-768	16
α-helix	775-779	5
β-strand	783	1	22
β-strand	785	1	22
α-helix	789-799	11
α-helix	806-814	9
α-helix	819-835	17
α-helix	843-854	12
α-helix	862-871	10
α-helix	877-891	15
α-helix	896-905	10
α-helix	917-927	11
α-helix	935-940	6
α-helix	949-953	5
α-helix	959-976	18
α-helix	982-990	9
α-helix	993-997	5
α-helix	1001-1011	11
α-helix	1020-1032	13
α-helix	1035-1037	3
α-helix	1046-1051	6
α-helix	1065-1080	16
α-helix	1092-1094	3
α-helix	1100-1114	15
α-helix	1120-1131	12
α-helix	1139-1141	3
α-helix	1142-1153	12
α-helix	1158-1161	4
α-helix	1163-1172	10
α-helix	1181-1183	3
α-helix	1187-1190	4
α-helix	1204-1218	15
α-helix	1221-1224	4
α-helix	1226-1233	8
α-helix	1242-1252	11
α-helix	1253-1255	3
α-helix	1256-1262	7
α-helix	1264-1282	19
α-helix	1291-1301	11
α-helix	1302-1306	5
α-helix	1311-1319	9
α-helix	1323-1329	7
α-helix	1330-1332	3

Chain 2: 50 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	5-12	8
α-helix	20-30	11
α-helix	41-51	11
α-helix	56-57	2
α-helix	58-69	12
α-helix	77-83	7
α-helix	93-105	13
α-helix	117-147	31
α-helix	156-162	7
α-helix	167-170	4
α-helix	175-203	29
α-helix	209-222	14
α-helix	242-253	12
α-helix	260-274	15
α-helix	278-294	17
α-helix	302-307	6
α-helix	308-312	5
α-helix	313-322	10
α-helix	330-342	13
α-helix	345-355	11
α-helix	359-369	11
α-helix	375-391	17
α-helix	406-420	15
α-helix	434-442	9
α-helix	448-457	10
α-helix	461-475	15
α-helix	483-505	23
α-helix	524-531	8
α-helix	553-561	9
α-helix	577-594	18
α-helix	599-611	13
α-helix	615-629	15
α-helix	634-636	3
α-helix	637-646	10
α-helix	660-674	15
α-helix	677-679	3
α-helix	707-720	14
α-helix	727-737	11
α-helix	741-752	12
α-helix	758-771	14
α-helix	776-782	7
α-helix	783-787	5
α-helix	788-792	5
α-helix	797-801	5
α-helix	803-822	20
α-helix	896-912	17
β-strand	916-917	2	20
α-helix	919-933	15
α-helix	953-958	6
α-helix	966-970	5
α-helix	976-986	11

Chain 3: 9 helices, 6 β-strands

Element	Residues	Length	Sheet
β-strand	18-23	6	32
α-helix	28-35	8
α-helix	36-40	5
α-helix	41-47	7
β-strand	66-71	6	32
β-strand	82-88	7	32
α-helix	91-98	8
α-helix	114-132	19
α-helix	138-140	3
β-strand	141-145	5	32
α-helix	149-150	2
α-helix	166-175	10
β-strand	179-184	6	32
α-helix	189-196	8
β-strand	220-222	3	32

Chain a: 14 helices, 24 β-strands

Element	Residues	Length	Sheet
β-strand	53	1	31
β-strand	55	1	69
β-strand	60	1	70
β-strand	61-66	6	71
α-helix	70-72	3
α-helix	80-98	19
β-strand	103	1	70
β-strand	104-110	7	71
β-strand	113-121	9	71
α-helix	124-134	11
α-helix	145-155	11
β-strand	166-171	6	71
α-helix	184-191	8
β-strand	195-200	6	71
α-helix	206-215	10
β-strand	219-221	3	71
α-helix	225-234	10
α-helix	238-239	2
β-strand	240	1	69
α-helix	241	1
β-strand	251-253	3	72
α-helix	255-256	2
β-strand	257-258	2	73
β-strand	270	1	74
β-strand	285	1	74
β-strand	288-289	2	73
β-strand	298	1	73
β-strand	304	1	72
β-strand	311-312	2	72
α-helix	319-321	3
β-strand	332-334	3	75
β-strand	344	1	76
β-strand	351	1	76
β-strand	357-359	3	75
β-strand	366-367	2	75
α-helix	369-374	6
α-helix	375-379	5
α-helix	383-386	4

Chain A: 69 helices, 65 β-strands

Element	Residues	Length	Sheet
α-helix	16-18	3
β-strand	20-25	6	41
α-helix	28-34	7
β-strand	47	1	42
β-strand	52	1	42
β-strand	86-95	10	43
α-helix	97-99	3
α-helix	100-107	8
α-helix	124-131	8
α-helix	137-147	11
α-helix	148-150	3
β-strand	153	1	44
β-strand	187	1	44
β-strand	190-193	4	45
β-strand	198-201	4	45
β-strand	214-215	2	45
α-helix	218-226	9
α-helix	230-236	7
α-helix	245-248	4
β-strand	249-255	7	43
α-helix	275-296	22
α-helix	300-316	17
α-helix	349-355	7
β-strand	358-359	2	46
β-strand	362-369	8	47
β-strand	377-379	3	47
α-helix	382-387	6
β-strand	389-393	5	48
α-helix	399-408	10
β-strand	416-420	5	48
β-strand	426-428	3	48
α-helix	435-437	3
β-strand	445-449	5	48
β-strand	455-459	5	47
α-helix	466-468	3
β-strand	471-474	4	47
β-strand	482-484	3	47
α-helix	489-492	4
β-strand	500-504	5	47
α-helix	509-518	10
β-strand	520	1	47
α-helix	521-524	4
β-strand	526	1	49
β-strand	533	1	49
α-helix	539-548	10
β-strand	554-555	2	50
α-helix	557-564	8
α-helix	574-577	4
β-strand	579-581	3	51
β-strand	585	1	51
β-strand	586-587	2	50
α-helix	588-593	6
β-strand	602-605	4	52
α-helix	611-614	4
β-strand	627-631	5	52
β-strand	634-637	4	52
α-helix	642-645	4
α-helix	652-683	32
β-strand	686	1	53
α-helix	696-721	26
α-helix	733-758	26
α-helix	765-772	8
α-helix	778-784	7
β-strand	789	1	54
β-strand	793	1	55
β-strand	796	1	55
α-helix	798-799	2
α-helix	811	1
β-strand	823	1	54
α-helix	833-856	24
α-helix	859-865	7
β-strand	879-880	2	56
β-strand	886-888	3	56
α-helix	891-893	3
β-strand	896	1	57
β-strand	902-905	4	58
α-helix	913-920	8
α-helix	927-930	4
β-strand	934	1	59
α-helix	939-942	4
α-helix	946-969	24
β-strand	976-979	4	58
α-helix	983-993	11
β-strand	1002	1	59
α-helix	1006-1017	12
α-helix	1028-1037	10
α-helix	1039-1048	10
α-helix	1053-1056	4
α-helix	1062-1079	18
β-strand	1080	1	57
α-helix	1087-1099	13
α-helix	1121-1128	8
β-strand	1139-1140	2	60
β-strand	1141-1142	2	61
β-strand	1143	1	62
α-helix	1145-1147	3
α-helix	1151-1157	7
β-strand	1164	1	63
α-helix	1166-1169	4
β-strand	1170-1177	8	64
α-helix	1191-1195	5
α-helix	1204-1206	3
β-strand	1207-1216	10	64
α-helix	1218-1223	6
α-helix	1228-1239	12
β-strand	1243	1	65
β-strand	1246-1247	2	64
α-helix	1250-1252	3
β-strand	1257-1260	4	64
β-strand	1261	1	65
β-strand	1262	1	64
α-helix	1281-1287	7
α-helix	1291-1295	5
β-strand	1299	1	63
β-strand	1310-1312	3	61
β-strand	1320-1322	3	66
β-strand	1328-1330	3	66
β-strand	1334-1336	3	61
α-helix	1343-1346	4
β-strand	1352	1	62
β-strand	1358-1359	2	60
α-helix	1362-1369	8
α-helix	1371-1387	17
α-helix	1395-1405	11
β-strand	1408	1	67
α-helix	1416-1419	4
α-helix	1426-1432	7
α-helix	1435-1445	11
β-strand	1448-1449	2	41
α-helix	1454-1459	6
α-helix	1467-1469	3
β-strand	1472-1475	4	68
α-helix	1478-1481	4
α-helix	1653-1656	4

Chain b: 13 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	10-16	7	103
α-helix	19-27	9
α-helix	34-51	18
β-strand	56-62	7	103
β-strand	67-71	5	103
α-helix	102-119	18
α-helix	134-149	16
β-strand	159-165	7	103
α-helix	168-170	3
α-helix	171-173	3
α-helix	174-186	13
β-strand	190-195	6	103
α-helix	201-209	9
β-strand	213-216	4	103
α-helix	220-222	3
α-helix	223-226	4
α-helix	227-231	5
α-helix	235-238	4
α-helix	244-250	7
β-strand	254	1	104
β-strand	261	1	104
β-strand	264-267	4	72
β-strand	274-275	2	72
β-strand	282	1	105
β-strand	287	1	105

Chain B: 49 helices, 79 β-strands

Element	Residues	Length	Sheet
α-helix	22-37	16
α-helix	41-49	9
α-helix	50-54	5
α-helix	55-60	6
β-strand	65-68	4	77
β-strand	83-93	11	77
β-strand	97-99	3	78
β-strand	105-107	3	78
α-helix	110-115	6
β-strand	121-133	13	77
β-strand	140	1	77
β-strand	143-153	11	77
α-helix	168-173	6
β-strand	185-187	3	79
β-strand	190-194	5	79
β-strand	196-200	5	80
α-helix	201	1
β-strand	206-208	3	81
β-strand	215-220	6	81
β-strand	236-240	5	81
β-strand	256-260	5	81
β-strand	263-268	6	81
α-helix	269-276	8
α-helix	281-288	8
α-helix	295-300	6
α-helix	302-306	5
α-helix	314-322	9
α-helix	332-341	10
α-helix	342-346	5
α-helix	358-377	20
α-helix	381-383	3
α-helix	388-390	3
β-strand	391-394	4	80
α-helix	397-424	28
α-helix	431-434	4
α-helix	438-450	13
β-strand	452	1	82
α-helix	460-462	3
β-strand	464	1	82
β-strand	467-469	3	79
α-helix	470-471	2
α-helix	475-481	7
β-strand	484	1	83
β-strand	485-486	2	80
α-helix	501-502	2
α-helix	503-505	3
β-strand	509	1	84
β-strand	525	1	83
β-strand	526	1	84
β-strand	531-532	2	85
α-helix	539-547	9
β-strand	551	1	86
α-helix	558-563	6
β-strand	566-569	4	87
β-strand	572-573	2	87
β-strand	576	1	86
α-helix	581-591	11
β-strand	602-606	5	87
β-strand	611-615	5	87
β-strand	621-622	2	85
β-strand	623-627	5	88
β-strand	628	1	89
β-strand	633	1	89
α-helix	637-644	8
α-helix	653-658	6
β-strand	662-665	4	88
α-helix	667-670	4
β-strand	675-676	2	88
β-strand	695-696	2	88
α-helix	700-703	4
α-helix	719-728	10
β-strand	732	1	90
β-strand	750-751	2	91
β-strand	759-760	2	92
α-helix	764-767	4
β-strand	776-782	7	93
β-strand	783	1	53
β-strand	793-797	5	93
α-helix	798-802	5
β-strand	808-817	10	91
β-strand	820	1	94
β-strand	826	1	94
β-strand	829	1	95
β-strand	844	1	96
β-strand	853	1	97
α-helix	854-855	2
β-strand	859-860	2	91
β-strand	864	1	96
β-strand	865-867	3	97
β-strand	869	1	95
α-helix	871	1
β-strand	872	1	98
α-helix	873	1
β-strand	889	1	98
β-strand	894-895	2	97
β-strand	902-911	10	91
β-strand	917-927	11	91
β-strand	934-937	4	93
β-strand	942-949	8	93
β-strand	956	1	99
β-strand	965-967	3	93
α-helix	969-971	3
α-helix	978-993	16
β-strand	997-998	2	92
α-helix	1008-1016	9
β-strand	1025-1026	2	93
β-strand	1029	1	99
α-helix	1035	1
β-strand	1036	1	99
α-helix	1037	1
α-helix	1040	1
β-strand	1041-1050	10	93
β-strand	1051	1	90
α-helix	1055-1057	3
β-strand	1060-1062	3	47
β-strand	1067	1	100
β-strand	1074	1	100
β-strand	1084-1085	2	46
α-helix	1088-1097	10
α-helix	1100-1104	5
α-helix	1105-1109	5
β-strand	1115-1119	5	101
β-strand	1127	1	102
β-strand	1137	1	102
β-strand	1142	1	102
β-strand	1146-1150	5	101
α-helix	1153-1163	11
β-strand	1167-1172	6	41

46 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 2 of Mediator of RNA polymerase II transcription subunit 16	0	protein	841	Homo sapiens	Q9Y2X0 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 23	1	protein	1368	Homo sapiens	Q9ULK4 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 24	2	protein	989	Homo sapiens	O75448 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 25	3	protein	747	Homo sapiens	Q71SY5 (AlphaFold model)
DNA-directed RNA polymerase II subunit RPB1	A	protein	1970	Homo sapiens	P24928
DNA-directed RNA polymerase II subunit RPB2	B	protein	1174	Homo sapiens	P30876
DNA-directed RNA polymerase II subunit RPB3	C	protein	275	Homo sapiens	P19387
DNA-directed RNA polymerase II subunit RPB4	D	protein	142	Homo sapiens	O15514
DNA-directed RNA polymerase II subunit RPB5	E	protein	210	Homo sapiens	P19388
DNA-directed RNA polymerases I, II, and III subunit RPABC2	F	protein	127	Homo sapiens	P61218
DNA-directed RNA polymerase II subunit RPB7	G	protein	172	Homo sapiens	P62487
DNA-directed RNA polymerases I, II, and III subunit RPABC3	H	protein	150	Homo sapiens	P52434

44 more molecules are not listed.

Sequence of entity 1 (0), FASTA

>7LBM_1 Isoform 2 of Mediator of RNA polymerase II transcription subunit 16 (chains 0)
MCDLRRPAAGGMMDLAYVCEWEKWSKSTHCPSVPLACAWSCRNLIAFTMDLRSDDQDLTR
MIHILDTEHPWDLHSIPSEHHEAITCLEWDQSGSRLLSADADGQIKCWSMADHLANSWES
SVGSLVEGDPIVALSWLHNGVKLALHVEKSGASSFGEKFSRVKFSPSLTLFGGKPMEGWI
AVTVSGLVTVSLLKPSGQVLTSTESLCRLRGRVALADIAFTGGGNIVVATADGSSASPVQ
FYKVCVSVVSEKCRIDTEILPSLFMRCTTDLNRKDKFPAITHLKFLARDMSEQVLLCASS
QTSSIVECWSLRKEGLPVNNIFQQISPVVGDKQPTILKWRILSATNDLDRVSAVALPKLP
ISLTNTDLKVASDTQFYPGLGLALAFHDGSVHIVHRLSLQTMAVFYSSAAPRPVDEPAMK
RPRTAGPAVHLKAMQLSWTSLALVGIDSHGKLSVLRLSPSMGHPLEVGLALRHLLFLLEY
CMVTGYDWWDILLHVQPSMVQSLVEKLHEEYTRQTAALQQVLSTRILAMKASLCKLSPCT
VTRVCDYHTKLFLIAISSTLKSLLRPHFLNTPDKSPGDRLTEICTKITDVDIDKVMINLK
TEEFVLDMNTLQALQQLLQWVGDFVLYLLASLPNQGSLLRPGHSFLRDGTSLGMLRELMV
VIRIWGLLKPSCLPVYTATSDTQDSMSLLFRLLTKLWICCRDEGPASEPDEALVDECCLL
PSQLLIPSLDWLPASDGLVSRLQPKQPLRLQFGRAPTLPGSAATLQLDGLARAPGQPKID
HLRRLHLGACPTEECKACTRCGCVTMLKSPNRTTAVKQWEQRWIKNCLCGGLWWRVPLSY
P

Sequence of entity 2 (1), FASTA

>7LBM_2 Mediator of RNA polymerase II transcription subunit 23 (chains 1)
METQLQSIFEEVVKTEVIEEAFPGMFMDTPEDEKTKLISCLGAFRQFWGGLSQESHEQCI
QWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRLVCESLINSDTLEWERTQLWALTFK
LVRKIIGGVDYKGVRDLLKVILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPA
YFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNS
WKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVL
EDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKL
AGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKEYIPVPDINKPQST
HAFAMTCIWIHLNRKAQNDNSKLQIPIPHSLRLHHEFLQQSLRNKSLQMNDYKIALLCNA
YSTNSECFTLPMGALVETIYGNGIMRIPLPGTNCMASGSITPLPMNLLDSLTVHAKMSLI
HSIATRVIKLAHAKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGI
LHTLLEMFSYRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSE
VQPQFTRFLSDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIM
SFTPHNWASHTLSCFPGPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHF
SMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGG
QQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRV
SDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVC
LRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRA
FLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREENPWVPDDTYYCRLIGRLVDTMA
GKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVSGKEVGNALLNVVLKSQPLVPRE
NITAWMNAIGLIITALPEPYWIVLHDRIVSVISSPSLTSETEWVGYPFRLFDFTACHQSY
SEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEVLLPIVKTEFQLLYVYHLVGPFLQRFQ
QERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICN
LKPALKLRLRFITHISKMEPAAVPPQAMNSGSPAPQSNQVPVSLPVTQ

Sequence of entity 3 (2), FASTA

>7LBM_3 Mediator of RNA polymerase II transcription subunit 24 (chains 2)
MKVVNLKQAILQAWKERWSDYQWAINMKKFFPKGATWDILNLADALLEQAMIGPSPNPLI
LSYLKYAISSQMVSYSSVLTAISKFDDFSRDLCVQALLDIMDMFCDRLSCHGKAEECIGL
CRALLSALHWLLRCTAASAERLREGLEAGTPAAGEKQLAMCLQRLEKTLSSTKNRALLHI
AKLEEASSWTAIEHSLLKLGEILANLSNPQLRSQAEQCGTLIRSIPTMLSVHAEQMHKTG
FPTVHAVILLEGTMNLTGETQSLVEQLTMVKRMQHIPTPLFVLEIWKACFVGLIESPEGT
EELKWTAFTFLKIPQVLVKLKKYSHGDKDFTEDVNCAFEFLLKLTPLLDKADQRCNCDCT
NFLLQECGKQGLLSEASVNNLMAKRKADREHAPQQKSGENANIQPNIQLILRAEPTVTNI
LKTMDADHSKSPEGLLGVLGHMLSGKSLDLLLAAAAATGKLKSFARKFINLNEFTTYGSE
ESTKPASVRALLFDISFLMLCHVAQTYGSEVILSESRTGAEVPFFETWMQTCMPEEGKIL
NPDHPCFRPDSTKVESLVALLNNSSEMKLVQMKWHEACLSISAAILEILNAWENGVLAFE
SIQKITDNIKGKVCSLAVCAVAWLVAHVRMLGLDEREKSLQMIRQLAGPLFSENTLQFYN
ERVVIMNSILERMCADVLQQTATQIKFPSTGVDTMPYWNLLPPKRPIKEVLTDIFAKVLE
KGWVDSRSIHIFDTLLHMGGVYWFCNNLIKELLKETRKEHTLRAVELLYSIFCLDMQQVT
LVLLGHILPGLLTDSSKWHSLMDPPGTALAKLAVWCALSSYSSHKGQASTRQKKRHREDI
EDYISLFPLDDVQPSKLMRLLSSNEDDANILSSPTDRSMSSSLSASQLHTVNMRDPLNRV
LANLFLLISSILGSRTAGPHTQFVQWFMEECVDCLEQGGRGSVLQFMPFTTVSELVKVSA
MSSPKVVLAITDLSLPLGRQVAAKAIAAL

Sequence of entity 4 (3), FASTA

>7LBM_4 Mediator of RNA polymerase II transcription subunit 25 (chains 3)
MVPGSEGPARAGSVVADVVFVIEGTANLGPYFEGLRKHYLLPAIEYFNGGPPAETDFGGD
YGGTQYSLVVFNTVDCAPESYVQCHAPTSSAYEFVTWLDGIKFMGGGGESCSLIAEGLST
ALQLFDDFKKMREQIGQTHRVCLLICNSPPYLLPAVESTTYSGCTTENLVQQIGERGIHF
SIVSPRKLPALRLLFEKAAPPALLEPLQPPTDVSQDPRHMVLVRGLVLPVGGGSAPGPLQ
SKQPVPLPPAAPSGATLSAAPQQPLPPVPPQYQVPGNLSAAQVAAQNAVEAAKNQKAGLG
PRFSPITPLQQAAPGVGPPFSQAPAPQLPPGPPGAPKPPPASQPSLVSTVAPGSGLAPTA
QPGAPSMAGTVAPGGVSGPSPAQLGAPALGGQQSVSNKLLAWSGVLEWQEKPKPASVDAN
TKLTRSLPCQVYVNHGENLKTEQWPQKLIMQLIPQQLLTTLGPLFRNSRMVQFHFTNKDL
ESLKGLYRIMGNGFAGCVHFPHTAPCEVRVLMLLYSSKKKIFMGLIPYDQSGFVNGIRQV
ITNHKQVQQQKLEQQQRGMGGQQAPPGLGPILEDQARPSQNLLQLRPPQPQPQGTVGASG
ATGQPQPQGTAQPPPGAPQGPPGAASGPPPPGPILRPQNPGANPQLRSLLLNPPPPQTGV
PPPQASLHHLQPPGAPALLPPPHQGLGQPQLGPPLLHPPPAQSWPAQLPPRAPLPGQMLL
SGGPRGPVPQPGLQPSVMEDDILMDLI

Sequence of entity 5 (A), FASTA

>7LBM_5 DNA-directed RNA polymerase II subunit RPB1 (chains A)
MHGGGPPSGDSACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDP
RQGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVCFFCSKLLVD
SNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGEEMDNKFGVEQPEGDEDLTKEKG
HGGCGRYQPRIRRSGLELYAEWKHVNEDSQEKKILLSPERVHEIFKRISDEECFVLGMEP
RYARPEWMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQNGAAA
HVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGKEGRVRGNLMGKRVD
FSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTPFNIDRLQELVRRGNSQYPGAKYII
RDNGDRIDLRFHPKPSDLHLQTGYKVERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWS
TFRLNLSVTTPYNADFDGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQD
TLTAVRKFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLIIPGHI
NCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLGTSAGSLVHISYLEM
GHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADSKTYQDIQNTIKKAKQDVIEVIEKA
HNNELEPTPGNTLRQTFENQVNRILNDARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKIN
ISQVIAVVGQQNVEGKRIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHA
MGGREGLIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGLAGES
VEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNAHIQNELEREFERMR
EDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHINPRLPSDLHPIKVVEGVKELSKKL
VIVNGDDPLSRQAQENATLLFNIHLRSTLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAI
AHPGEMVGALAAQSLGEPATQMTLNTFHYAGVSAKNVTLGVPRLKELINISKKPKTPSLT
VFLLGQSARDAERAKDILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMP
DFDVARISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKLVLRIR
IMNSDENKMQEEEEVVDKMDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKKI
IITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVLGIEAVRKALER
ELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGVNRQDTGPLMKCSFEETVDVLM
EAAAHGESDPMKGVSENIMLGQLAPAGTGCFDLLLDAEKCKYGMEIPTNIPGLGAAGPTG
MFFGSAPSPMGGISPAMTPWNQGATPAYGAWSPSVGSGMTPGAAGFSPSAASDASGFSPG
YSPAWSPTPGSPGSPGPSSPYIPSPGGAMSPSYSPTSPAYEPRSPGGYTPQSPSYSPTSP
SYSPTSPSYSPTSPNYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSP
TSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPS
YSPTSPNYSPTSPNYTPTSPSYSPTSPSYSPTSPNYTPTSPNYSPTSPSYSPTSPSYSPT
SPSYSPSSPRYTPQSPTYTPSSPSYSPSSPSYSPASPKYTPTSPSYSPSSPEYTPTSPKY
SPTSPKYSPTSPKYSPTSPTYSPTTPKYSPTSPTYSPTSPVYTPTSPKYSPTSPTYSPTS
PKYSPTSPTYSPTSPKGSTYSPTSPGYSPTSPTYSLTSPAISPDDSDEEN

Sequence of entity 6 (B), FASTA

>7LBM_6 DNA-directed RNA polymerase II subunit RPB2 (chains B)
MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVE
DAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTY
SAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLD
PGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSML
ARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEM
VKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETK
KAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQ
KFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLS
HLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSP
ILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSE
VSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASG
VVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSP
RNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIV
AIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGM
RHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSE
TGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEG
ITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGY
HLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSR
DGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGC
RNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Sequence of entity 7 (C), FASTA

>7LBM_7 DNA-directed RNA polymerase II subunit RPB3 (chains C)
MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLH
DEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDL
ISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTA
GVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLR
PETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

Sequence of entity 8 (D), FASTA

>7LBM_8 DNA-directed RNA polymerase II subunit RPB4 (chains D)
MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEV
FMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEG
RFEDEELQQILDDIQTKRSFQY

Sequence of entity 9 (E), FASTA

>7LBM_9 DNA-directed RNA polymerase II subunit RPB5 (chains E)
MDDEEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQSGDKPSEGRPRRTDLTV
LVAHNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVD
MAPKYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQLPRIQAGDPVA
RYFGIKRGQVVKIIRPSETAGRYITYRLVQ

Sequence of entity 10 (F), FASTA

>7LBM_10 DNA-directed RNA polymerases I, II, and III subunit RPABC2 (chains F)
MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKY
ERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGV
DELIITD

Sequence of entity 11 (G), FASTA

>7LBM_11 DNA-directed RNA polymerase II subunit RPB7 (chains G)
MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ
PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD
PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS

Sequence of entity 12 (H), FASTA

>7LBM_12 DNA-directed RNA polymerases I, II, and III subunit RPABC3 (chains H)
MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVI
ASTLYEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGG
LLMRLQGDANNLHGFEVDSRVYLLMKKLAF

Ligands and cofactors

ID	Name	Formula	Copies
MG	Magnesium ion	Mg	2
ZN	Zinc ion	Zn	16
SF4	Iron/sulfur cluster	Fe4 S4	1

Primary citation

Structure of the human Mediator-bound transcription preinitiation complex. Abdella, R., Talyzina, A., Chen, S. et al. Science (2021) 372:52-56. DOI 10.1126/science.abg3074 · PubMed

Other PDB entries of the same protein (UniProt Q9Y2X0 (AlphaFold model), which also has an AlphaFold model), best resolution first:

7EMF 3.5 Å, Human Mediator (deletion of MED1-IDR) in a Tail-extended conformation
8TRH 3.7 Å, The IDRc bound human core Mediator complex
7ENA 4.07 Å, TFIID-based PIC-Mediator holo-complex in pre-assembled state (pre-hPIC-MED)
7ENC 4.13 Å, TFIID-based PIC-Mediator holo-complex in fully-assembled state (hPIC-MED)
8GXS 4.16 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in H-binding state
7ENJ 4.4 Å, Human Mediator (deletion of MED1-IDR) in a Tail-bent conformation (MED-B)
8T9D 4.66 Å, CryoEM structure of TR-TRAP
8GXQ 5.04 Å, PIC-Mediator in complex with +1 nucleosome (T40N) in MH-binding state
8TQW 8.2 Å, Structure of human transcriptional Mediator complex

Browse structure collections

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