7LJ5: PDB entry 7LJ5

Human TRAAK K+ channel FHIEG mutant A198E in a K+ bound conductive conformation. Determined by X-ray diffraction at 2.26 Å resolution. Released 30 Jun 2021.

Method: X-ray diffraction
Resolution: 2.26 Å
Organisms: Homo sapiens, Mus musculus
Chains: 6
Atoms: 10,660
Mol. weight: 158.67 kDa
Ligands: CA
Released: 30 Jun 2021

Explore 7LJ5 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7LJ5 contains 49 α-helices and 86 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 0 β-strands

Element	Residues	Length
α-helix	29-54	26
α-helix	55-57	3
α-helix	60-75	16
α-helix	81-96	16
α-helix	116-127	12
α-helix	140-184	45
α-helix	190-206	17
α-helix	207-211	5
α-helix	212-221	10
α-helix	225-236	12
α-helix	257-285	29

Chain B: 9 helices, 0 β-strands

Element	Residues	Length
α-helix	29-75	47
α-helix	81-96	16
α-helix	116-127	12
α-helix	140-185	46
α-helix	190-206	17
α-helix	207-211	5
α-helix	212-221	10
α-helix	225-236	12
α-helix	257-286	30

Chain D: 7 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-5	2	1
β-strand	10-13	4	2
β-strand	19-25	7	1
β-strand	33-37	5	2
β-strand	44-48	5	2
β-strand	52-53	2	2
α-helix	54	1
β-strand	61-66	6	1
β-strand	69-74	6	1
α-helix	79-81	3
β-strand	83-89	7	2
α-helix	95	1
β-strand	96-97	2	2
β-strand	101-105	5	2
β-strand	110	1	3
β-strand	113-117	5	4
α-helix	118-120	3
α-helix	121-124	4
β-strand	128-138	11	4
β-strand	139	1	3
β-strand	144-149	6	5
β-strand	152-154	3	5
β-strand	158-162	5	4
α-helix	163-164	2
β-strand	172-181	10	4
α-helix	182-187	6
β-strand	190-196	7	5
β-strand	204-209	6	5

Chain E: 7 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	6
β-strand	10-12	3	7
β-strand	18-25	8	6
α-helix	29-31	3
β-strand	34-39	6	7
β-strand	46-51	6	7
β-strand	58-60	3	7
α-helix	62-64	3
β-strand	68-73	6	6
β-strand	78-83	6	6
α-helix	88-90	3
β-strand	92-98	7	7
β-strand	105-106	2	7
β-strand	110-114	5	7
β-strand	120	1	8
α-helix	121-122	2
β-strand	123-127	5	9
β-strand	138-148	11	9
β-strand	149	1	8
β-strand	154-157	4	10
α-helix	158-160	3
β-strand	162	1	10
β-strand	166-168	3	9
α-helix	169-171	3
β-strand	172-174	3	9
β-strand	177-187	11	9
β-strand	197-202	6	10
α-helix	203-205	3
β-strand	207-212	6	10

Chain F: 7 helices, 21 β-strands

Element	Residues	Length	Sheet
β-strand	4-6	3	11
β-strand	10-13	4	12
β-strand	19-25	7	11
β-strand	33-37	5	12
β-strand	44-48	5	12
β-strand	52-53	2	12
α-helix	54	1
β-strand	61-66	6	11
β-strand	69-74	6	11
α-helix	79-81	3
β-strand	83-89	7	12
α-helix	95	1
β-strand	96-97	2	12
β-strand	101-105	5	12
α-helix	106	1
β-strand	110	1	13
β-strand	113-117	5	14
α-helix	121-126	6
β-strand	128-138	11	14
β-strand	139	1	13
β-strand	144-149	6	15
β-strand	152-154	3	15
β-strand	158-162	5	14
α-helix	163-166	4
β-strand	172-181	10	14
α-helix	182-187	6
β-strand	190-196	7	15
β-strand	204-209	6	15

Chain G: 8 helices, 22 β-strands

Element	Residues	Length	Sheet
β-strand	3-6	4	16
β-strand	10-12	3	17
β-strand	18-25	8	16
α-helix	29-31	3
β-strand	34-39	6	17
α-helix	44-45	2
β-strand	46-51	6	17
β-strand	58-60	3	17
α-helix	62-64	3
β-strand	68-73	6	16
β-strand	78-83	6	16
α-helix	88-90	3
β-strand	92-98	7	17
β-strand	105-106	2	17
β-strand	110-114	5	17
β-strand	120	1	18
α-helix	121-122	2
β-strand	123-127	5	19
β-strand	138-148	11	19
β-strand	149	1	18
β-strand	154-157	4	20
α-helix	158-160	3
β-strand	162	1	20
β-strand	166-168	3	19
α-helix	169-171	3
β-strand	172-174	3	19
β-strand	177-187	11	19
β-strand	197-202	6	20
α-helix	203-205	3
β-strand	207-212	6	20

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 2 of Potassium channel subfamily K member 4	A, B	protein	299	Homo sapiens	Q9NYG8 (AlphaFold model)
Anti-traak antibody 13E9 FAB fragment light chain	D, F	protein	211	Mus musculus
Anti-traak antibody 13E9 FAB fragment heavy chain	E, G	protein	217	Mus musculus

Sequence of entity 1 (A, B), FASTA

>7LJ5_1 Isoform 2 of Potassium channel subfamily K member 4 (chains A, B)
MTTAPQEPPARPLQAGSGAGPAPGRAMRSTTLLALLALVLLYLVSGALVFRALEQPHEQQ
AQRELGEVREKFLRAHPCVSDQELGLLIKEVADALGGGADPETQSTSQSSHSAWDLGSAF
FFSGTIITTIGYGNVALRTDAGRLFCIFYALVGIPLFGILLAGVGDRLGSSLRHGIGHIE
AIFLKWHVPPELVRVLSEMLFLLIGCLLFVLTPTFVFCYMEDWSKLEAIYFVIVTLTTVG
FGDYVAGADPRQDSPAYQPLVWFWILLGLAYFASVLTTIGNWLRVVSRRTSNSLEVLFQ

Sequence of entity 2 (D, F), FASTA

>7LJ5_2 ANTI-TRAAK ANTIBODY 13E9 FAB FRAGMENT LIGHT CHAIN (chains D, F)
QIVLTQSPAIMSASPGEKVTMTCSASSSVSYMHWYQQKSGTSPKRWIYDTSKLASGVPAR
FSGSGSGTSYSLTISSMEAEDAATYYCQQWSNSPPTFGAGAKLELKRADAAPTVSIFPPS
SEQLTSGGASVVCFLNNFYPKDINVKWKIDGSERQNGVLNSWTDQDSKDSTYSMSSTLTL
TKDEYERHNSYTCEATHKTSTSPIVKSFNRN

Sequence of entity 3 (E, G), FASTA

>7LJ5_3 ANTI-TRAAK ANTIBODY 13E9 FAB FRAGMENT HEAVY CHAIN (chains E, G)
EVQLQQSGPELVKPGASMKTSCKVSGYSFTGYIMNWVKQRHGKNLEWIGLINPNTGYTTY
NQKFKGKATLTVDKSSSTAYMELLSLTSEDSAIYYCTRGNYVFDYWGQGTTLTVSSAKTT
PPSVYPLAPGSAAQTNSMVTLGCLVKGYFPEPVTVTWNSGSLSSGVHTFPAVLQSDLYTL
SSSVTVPSSSWPSETVTCNVAHPASSTKVDKKIVPRD

Ligands and cofactors

ID	Name	Formula	Copies
CA	Calcium ion	Ca	3

Water and common crystallization additives (K) are not listed.

Primary citation

Physical basis for distinct basal and mechanically gated activity of the human K + channel TRAAK. Rietmeijer, R.A., Sorum, B., Li, B. et al. Neuron (2021) 109:2902-2913.e4. DOI 10.1016/j.neuron.2021.07.009 · PubMed

Other PDB entries of the same protein (UniProt Q9NYG8 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4WFE 2.5 Å, Human TRAAK K+ channel in a K+ bound conductive conformation
4WFF 2.5 Å, Human TRAAK K+ channel in a K+ bound nonconductive conformation
4I9W 2.75 Å, Human two pore domain K+ channel TRAAK (K2P4.1) - Fab complex structure
7LJA 2.77 Å, Human TRAAK K+ channel FHEIG mutant A198E in a Tl+ bound conductive conformation
7LJ4 2.78 Å, Human TRAAK K+ channel FHEIG mutant A270P in a K+ bound conductive conformation
7LJB 2.97 Å, Human TRAAK K+ channel mutant G158D in a K+ bound conductive conformation
4WFG 3.0 Å, Human TRAAK K+ channel in a Tl+ bound conductive conformation
4WFH 3.01 Å, Human TRAAK K+ channel in a Tl+ bound nonconductive conformation
4RUE 3.3 Å, Human K2P4.1 (TRAAK) potassium channel, G124I mutant
3UM7 3.31 Å, Crystal structure of the human two pore domain K+ ion channel TRAAK (K2P4.1)
4RUF 3.4 Å, Human K2P4.1 (TRAAAK) potassium channel, W262S mutant

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