7PFO: Core human replisome

Name: PDB 7PFO: Core human replisome
Published: 2021-11-10
License: https://creativecommons.org/publicdomain/zero/1.0/
Keywords: replication

Core human replisome. Determined by electron microscopy at 3.2 Å resolution. Released 10 Nov 2021.

Method: Electron microscopy
Resolution: 3.2 Å
Organism: Homo sapiens
Chains: 21
Atoms: 68,517
Mol. weight: 1797.09 kDa
Ligands: ZN, MG, ANP
Released: 10 Nov 2021

Explore 7PFO in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7PFO contains 421 α-helices and 367 β-strands across 19 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain 2: 41 helices, 30 β-strands

Element	Residues	Length	Sheet
α-helix	170-172	3
α-helix	182-187	6
α-helix	189-205	17
β-strand	207	1	1
β-strand	213	1	1
α-helix	214-225	12
β-strand	229-233	5	2
α-helix	234-240	7
α-helix	242-247	6
α-helix	252-270	19
α-helix	272-276	5
β-strand	282-286	5	2
β-strand	291	1	3
α-helix	294-296	3
α-helix	299-301	3
β-strand	305-315	11	3
α-helix	316-318	3
β-strand	319-328	10	3
α-helix	334	1
β-strand	335-340	6	3
β-strand	351	1	4
β-strand	358	1	4
β-strand	362-363	2	3
β-strand	369-379	11	3
α-helix	390-392	3
β-strand	393-399	7	3
α-helix	400-402	3
β-strand	411-422	12	3
α-helix	424-430	7
β-strand	434	1	5
β-strand	435-446	12	3
α-helix	453-455	3
α-helix	458-467	10
α-helix	473-480	8
α-helix	489-500	12
β-strand	505	1	6
α-helix	507-509	3
β-strand	513	1	6
β-strand	519-523	5	7
α-helix	529-539	11
β-strand	544-547	4	7
α-helix	548-550	3
β-strand	551	1	8
α-helix	553-557	5
β-strand	558-562	5	9
β-strand	569-573	5	9
α-helix	575-578	4
β-strand	583-587	5	7
α-helix	589-591	3
α-helix	594-597	4
α-helix	598-600	3
α-helix	601-605	5
β-strand	608-613	6	10
β-strand	616-621	6	10
β-strand	625-630	6	7
α-helix	643-647	5
α-helix	651-654	4
β-strand	659-663	5	7
α-helix	669-685	17
α-helix	714-716	3
α-helix	717-730	14
β-strand	734	1	11
α-helix	740-755	16
α-helix	763-780	18
β-strand	785	1	11
α-helix	788-804	17
α-helix	811-821	11
α-helix	829-848	20
α-helix	861-871	11
α-helix	878-881	4
α-helix	885-888	4

Chain 3: 33 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	12-23	12
α-helix	31-42	12
β-strand	46-50	5	12
α-helix	51-57	7
α-helix	59-67	9
α-helix	69-87	19
α-helix	89-93	5
β-strand	99-103	5	12
α-helix	107-109	3
β-strand	110-111	2	13
α-helix	118-120	3
β-strand	124-134	11	13
α-helix	135-137	3
β-strand	138-148	11	13
β-strand	153-158	6	13
β-strand	179	1	14
β-strand	182	1	14
β-strand	185-202	18	13
α-helix	205-207	3
α-helix	212-215	4
β-strand	216-222	7	13
α-helix	223-225	3
β-strand	234-244	11	13
β-strand	251-252	2	15
β-strand	255-256	2	16
β-strand	257-267	11	13
α-helix	282-291	10
α-helix	295-301	7
α-helix	311-322	12
β-strand	327-328	2	17
β-strand	334-335	2	17
β-strand	341-345	5	18
α-helix	351-361	11
β-strand	365-369	5	18
α-helix	370-372	3
β-strand	373	1	19
α-helix	375-379	5
β-strand	380-384	5	20
β-strand	391-395	5	20
α-helix	397-400	4
β-strand	404-409	6	18
α-helix	411-413	3
α-helix	416-428	13
β-strand	430-435	6	21
β-strand	438-443	6	21
β-strand	446-452	7	18
α-helix	453-454	2
α-helix	459-460	2
α-helix	465-468	4
α-helix	473-476	4
β-strand	481-485	5	18
α-helix	491-506	16
β-strand	516	1	22
α-helix	517-521	5
β-strand	553	1	23
α-helix	562	1
β-strand	563	1	23
α-helix	564	1
α-helix	565-575	11
β-strand	581-582	2	24
α-helix	584-603	20
α-helix	615-632	18
β-strand	636-637	2	24
α-helix	639-654	16

Chain 4: 30 helices, 31 β-strands

Element	Residues	Length	Sheet
β-strand	150-152	3	25
β-strand	158	1	25
α-helix	159-172	14
α-helix	192-203	12
β-strand	207-211	5	26
α-helix	212-218	7
α-helix	220-228	9
α-helix	230-247	18
β-strand	259-263	5	26
α-helix	271-273	3
α-helix	276-278	3
β-strand	282-292	11	27
β-strand	296-306	11	27
β-strand	312-316	5	27
β-strand	321	1	28
β-strand	327	1	29
β-strand	334	1	29
β-strand	337-339	3	27
β-strand	345-355	11	27
α-helix	358-360	3
α-helix	362	1
α-helix	365-368	4
β-strand	369-375	7	27
β-strand	387-397	11	27
β-strand	400-402	3	30
β-strand	405-409	5	30
β-strand	411	1	31
α-helix	412	1
β-strand	413-423	11	27
α-helix	443-453	11
α-helix	458-465	8
α-helix	474-485	12
β-strand	506-509	4	32
α-helix	515-523	9
α-helix	524-526	3
β-strand	531-534	4	32
β-strand	546-550	5	33
β-strand	555-559	5	33
α-helix	562-565	4
β-strand	570-574	5	32
α-helix	576-578	3
α-helix	581-593	13
β-strand	595-598	4	34
β-strand	605-608	4	34
β-strand	612-617	6	32
α-helix	630-634	5
α-helix	638-641	4
β-strand	646-649	4	32
α-helix	651-653	3
α-helix	656-670	15
α-helix	674-697	24
β-strand	702	1	35
α-helix	704-719	16
α-helix	720-725	6
α-helix	731-747	17
β-strand	753	1	35
α-helix	755-772	18
β-strand	774-775	2	36
β-strand	779-780	2	36
α-helix	783-786	4
α-helix	791-794	4

Chain 5: 30 helices, 31 β-strands

Element	Residues	Length	Sheet
α-helix	22-41	20
β-strand	44-46	3	37
β-strand	50-52	3	37
α-helix	54-65	12
β-strand	69-73	5	38
α-helix	74-80	7
α-helix	82-90	9
α-helix	92-111	20
β-strand	125-129	5	38
α-helix	133-134	2
α-helix	142-144	3
β-strand	148-158	11	16
α-helix	159-161	3
β-strand	162-172	11	16
β-strand	178-183	6	16
α-helix	184-185	2
β-strand	191-192	2	15
α-helix	193-194	2
β-strand	212-214	3	16
β-strand	220-230	11	16
α-helix	231-232	2
α-helix	237	1
α-helix	240-243	4
β-strand	244-250	7	16
α-helix	251-253	3
β-strand	262-271	10	16
β-strand	289	1	3
β-strand	292-301	10	16
α-helix	316-327	12
α-helix	331-338	8
α-helix	347-358	12
β-strand	363-364	2	39
β-strand	370-371	2	39
β-strand	377-381	5	40
α-helix	387-397	11
β-strand	401-405	5	40
β-strand	409	1	10
α-helix	411-414	4
β-strand	416-420	5	41
β-strand	427-431	5	41
α-helix	433-436	4
β-strand	441-445	5	40
α-helix	447-449	3
α-helix	452-457	6
α-helix	459-463	5
β-strand	466-471	6	19
β-strand	474-479	6	19
β-strand	483-489	7	40
β-strand	490	1	42
β-strand	493	1	42
α-helix	500-503	4
α-helix	508-512	5
β-strand	516-520	5	40
α-helix	526-541	16
α-helix	556-569	14
β-strand	570	1	22
β-strand	573-574	2	43
α-helix	576-600	25
α-helix	610-626	17
β-strand	631-632	2	43
α-helix	634-649	16

Chain 6: 29 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	21-36	16
α-helix	45-50	6
α-helix	51-54	4
β-strand	60-64	5	44
α-helix	65-71	7
α-helix	73-81	9
α-helix	83-98	16
α-helix	99-101	3
β-strand	111-115	5	44
β-strand	121	1	5
α-helix	123-125	3
α-helix	128-130	3
β-strand	131	1	5
β-strand	134-144	11	5
α-helix	145-147	3
β-strand	148-158	11	5
β-strand	164-169	6	5
β-strand	191-193	3	5
β-strand	199-210	12	5
α-helix	215-216	2
α-helix	220-222	3
β-strand	223-229	7	5
α-helix	230-232	3
β-strand	241-252	12	5
α-helix	254-256	3
β-strand	281	1	45
β-strand	282	1	28
β-strand	292	1	45
β-strand	294-305	12	5
α-helix	322-328	7
α-helix	331-341	11
α-helix	346-354	9
α-helix	362-373	12
β-strand	378-379	2	46
β-strand	385-386	2	46
β-strand	392-396	5	47
α-helix	402-412	11
β-strand	417-420	4	47
β-strand	432-436	5	48
β-strand	441-445	5	48
α-helix	446	1
α-helix	448-451	4
β-strand	455-460	6	47
α-helix	467-478	12
β-strand	481-486	6	8
β-strand	489-494	6	8
β-strand	497-503	7	47
α-helix	516-519	4
α-helix	524-529	6
β-strand	532-536	5	47
α-helix	542-558	17
α-helix	569-579	11
β-strand	585-586	2	49
α-helix	588-606	19
α-helix	618-634	17
β-strand	639-640	2	49
α-helix	642-660	19

Chain 7: 35 helices, 24 β-strands

Element	Residues	Length	Sheet
α-helix	6-19	14
α-helix	24-27	4
α-helix	32-41	10
β-strand	47-51	5	50
α-helix	52-56	5
α-helix	60-68	9
α-helix	70-88	19
α-helix	98-110	13
α-helix	128-131	4
β-strand	135-139	5	50
α-helix	140-141	2
β-strand	147	1	31
α-helix	149-151	3
α-helix	154-156	3
β-strand	160-170	11	31
α-helix	171-173	3
β-strand	174-184	11	31
β-strand	190-194	5	31
β-strand	199-200	2	30
α-helix	201-203	3
β-strand	205	1	51
α-helix	209-212	4
β-strand	219	1	51
β-strand	220-222	3	31
β-strand	228-238	11	31
α-helix	241-243	3
α-helix	249-251	3
β-strand	252-258	7	31
α-helix	259-261	3
β-strand	270-279	10	31
α-helix	285-290	6
β-strand	293	1	13
β-strand	296-305	10	31
α-helix	321-328	8
α-helix	332-339	8
α-helix	348-359	12
β-strand	377-381	5	52
α-helix	387-397	11
β-strand	401-405	5	52
β-strand	417-419	3	53
β-strand	428-430	3	53
α-helix	433-436	4
β-strand	441-445	5	52
α-helix	447-449	3
α-helix	452-464	13
β-strand	466-472	7	54
β-strand	474-479	6	54
α-helix	482	1
β-strand	483-486	4	52
α-helix	501-505	5
α-helix	509-512	4
β-strand	517-521	5	52
α-helix	527-543	17
α-helix	546-548	3
α-helix	553-555	3
α-helix	556-567	12
α-helix	575-594	20
α-helix	603-619	17
α-helix	627-644	18

Chain A: 24 helices, 33 β-strands

Element	Residues	Length	Sheet
α-helix	3-16	14
β-strand	20-21	2	55
α-helix	23-32	10
α-helix	41-54	14
β-strand	61-62	2	55
α-helix	64-74	11
β-strand	86-90	5	56
α-helix	91-93	3
β-strand	97-100	4	57
β-strand	105-108	4	57
α-helix	109-111	3
α-helix	114-117	4
α-helix	123-142	20
α-helix	148-149	2
β-strand	166	1	58
α-helix	168-171	4
β-strand	176	1	58
β-strand	181-188	8	58
β-strand	196-199	4	58
β-strand	202-207	6	58
α-helix	213-214	2
β-strand	219	1	59
β-strand	223-231	9	58
β-strand	234-241	8	58
α-helix	242-247	6
α-helix	248-255	8
β-strand	258	1	60
β-strand	267	1	60
α-helix	273-279	7
β-strand	287-290	4	61
α-helix	298-310	13
β-strand	318-322	5	61
α-helix	332-350	19
α-helix	353-358	6
β-strand	360-364	5	61
β-strand	376	1	62
β-strand	378	1	63
α-helix	379-380	2
α-helix	382	1
α-helix	383-392	10
β-strand	396-398	3	61
β-strand	401	1	63
β-strand	403-407	5	56
β-strand	410-416	7	56
α-helix	419-425	7
β-strand	427	1	59
α-helix	430-432	3
α-helix	437-448	12
β-strand	450	1	64
β-strand	461	1	62
α-helix	463-469	7
β-strand	470	1	64
β-strand	477-481	5	56
β-strand	487-490	4	56
β-strand	495-498	4	56
β-strand	509-513	5	61
β-strand	518-522	5	61

Chain B: 37 helices, 33 β-strands

Element	Residues	Length	Sheet
β-strand	1331-1336	6	65
β-strand	1345-1349	5	65
β-strand	1356-1360	5	65
β-strand	1365-1366	2	66
α-helix	1388-1392	5
α-helix	1393-1395	3
β-strand	1407-1408	2	66
β-strand	1430	1	66
β-strand	1486-1492	7	67
β-strand	1499-1500	2	67
β-strand	1509-1511	3	68
α-helix	1524-1527	4
α-helix	1536-1539	4
α-helix	1548-1549	2
β-strand	1554-1556	3	68
α-helix	1564-1576	13
β-strand	1584-1588	5	67
β-strand	1589	1	69
β-strand	1590	1	67
α-helix	1601	1
α-helix	1610	1
β-strand	1611	1	69
α-helix	1612	1
α-helix	1624-1627	4
α-helix	1629-1634	6
α-helix	1635-1653	19
β-strand	1661	1	70
β-strand	1663-1664	2	70
α-helix	1667-1679	13
α-helix	1684-1686	3
α-helix	1699-1706	8
β-strand	1715	1	71
β-strand	1729-1731	3	71
α-helix	1734-1738	5
α-helix	1739-1741	3
α-helix	1789-1804	16
α-helix	1813-1824	12
α-helix	1834-1854	21
α-helix	1855-1858	4
β-strand	1862-1866	5	71
β-strand	1870-1874	5	71
α-helix	1880-1899	20
β-strand	1905-1906	2	71
β-strand	1913-1916	4	72
β-strand	1921-1925	5	72
β-strand	1977-1980	4	72
α-helix	1981-1985	5
α-helix	1989-1991	3
α-helix	1992-2014	23
α-helix	2051-2075	25
α-helix	2083-2086	4
β-strand	2093	1	73
α-helix	2100-2114	15
α-helix	2120-2132	13
β-strand	2148	1	73
β-strand	2152-2158	7	74
β-strand	2163-2168	6	74
α-helix	2174-2176	3
α-helix	2178-2180	3
α-helix	2181-2184	4
β-strand	2186	1	75
β-strand	2193	1	75
α-helix	2196-2215	20
β-strand	2219-2221	3	76
β-strand	2227	1	76
α-helix	2228	1
α-helix	2240-2241	2
β-strand	2242-2244	3	76
α-helix	2248-2265	18
α-helix	2268-2279	12

11 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
DNA replication licensing factor MCM2	2	protein	904	Homo sapiens	P49736 (AlphaFold model)
DNA replication licensing factor MCM3	3	protein	808	Homo sapiens	P25205 (AlphaFold model)
DNA replication licensing factor MCM4	4	protein	863	Homo sapiens	P33991 (AlphaFold model)
DNA replication licensing factor MCM5	5	protein	734	Homo sapiens	P33992 (AlphaFold model)
DNA replication licensing factor MCM6	6	protein	821	Homo sapiens	Q14566
DNA replication licensing factor MCM7	7	protein	719	Homo sapiens	P33993
DNA polymerase epsilon subunit 2	A	protein	527	Homo sapiens	P56282
DNA polymerase epsilon catalytic subunit A	B	protein	2286	Homo sapiens	Q07864
Cell division control protein 45 homolog	C	protein	569	Homo sapiens	O75419
DNA replication complex GINS protein PSF1	D	protein	196	Homo sapiens	Q14691
DNA replication complex GINS protein PSF2	E	protein	185	Homo sapiens	Q9Y248
DNA replication complex GINS protein PSF3	F	protein	216	Homo sapiens	Q9BRX5

7 more molecules are not listed.

Sequence of entity 1 (2), FASTA

>7PFO_1 DNA replication licensing factor MCM2 (chains 2)
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTE
GPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQR
DREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGH
SVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAR
EHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQL
HLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGP
FEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHN
NYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFAS
IAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVS
SRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSI
HEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDIL
CVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVL
KKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARI
HLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLV
AEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMI
LQQF

Sequence of entity 2 (3), FASTA

>7PFO_2 DNA replication licensing factor MCM3 (chains 3)
MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEK
RANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCF
LSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEE
NNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGT
YRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLA
KSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLC
TAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT
AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFD
LLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQ
DTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQ
DSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEK
EKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQV
HTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPF
SSVEIQAALSKMQDDNQVMVSEGIIFLI

Sequence of entity 3 (4), FASTA

>7PFO_3 DNA replication licensing factor MCM4 (chains 4)
MSSPASTPSRRGSRRGRATPAQTPRSEDARSSPSQRRRGEDSTSTGELQPMPTSPGVDLQ
SPAAQDVLFSSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLGS
AQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRFLQRFIDPLAKE
EENVGIDITEPLYMQRLGEINVIGEPFLNVNCEHIKSFDKNLYRQLISYPQEVIPTFDMA
VNEIFFDRYPDSILEHQIQVRPFNALKTKNMRNLNPEDIDQLITISGMVIRTSQLIPEMQ
EAFFQCQVCAHTTRVEMDRGRIAEPSVCGRCHTTHSMALIHNRSLFSDKQMIKLQESPED
MPAGQTPHTVILFAHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVSNVKSVYKTHIDVIH
YRKTDAKRLHGLDEEAEQKLFSEKRVELLKELSRKPDIYERLASALAPSIYEHEDIKKGI
LLQLFGGTRKDFSHTGRGKFRAEINILLCGDPGTSKSQLLQYVYNLVPRGQYTSGKGSSA
VGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAK
AGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLLLDPQDEAYDR
RLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKI
GSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGI
VDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMF
EEALRALADDDFLTVTGKTVRLL

Sequence of entity 4 (5), FASTA

>7PFO_4 DNA replication licensing factor MCM5 (chains 5)
MSGFDDPGIFYSDSFGGDAQADEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELK
RHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEV
LQDIQVMLKSDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLT
NIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGE
MPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQ
VDTDGSGRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGG
SRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMR
DPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNS
RCSVLAAANSVFGRWDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLH
VSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRLSAEAAEKLKNRYIIMRSGARQHERDS
DRRSSIPITVRQLEAIVRIAEALSKMKLQPFATEADVEEALRLFQVSTLDAALSGTLSGV
EGFTSQEDQEMLSRIEKQLKRRFAIGSQVSEHSIIKDFTKQKYPEHAIHKVLQLMLRRGE
IQHRMQRKVLYRLK

Sequence of entity 5 (6), FASTA

>7PFO_5 DNA replication licensing factor MCM6 (chains 6)
MDLAAAAEPGAGSQHLEVRDEVAEKCQKLFLDFLEEFQSSDGEIKYLQLAEELIRPERNT
LVVSFVDLEQFNQQLSTTIQEEFYRVYPYLCRALKTFVKDRKEIPLAKDFYVAFQDLPTR
HKIRELTSSRIGLLTRISGQVVRTHPVHPELVSGTFLCLDCQTVIRDVEQQFKYTQPNIC
RNPVCANRRRFLLDTNKSRFVDFQKVRIQETQAELPRGSIPRSLEVILRAEAVESAQAGD
KCDFTGTLIVVPDVSKLSTPGARAETNSRVSGVDGYETEGIRGLRALGVRDLSYRLVFLA
CCVAPTNPRFGGKELRDEEQTAESIKNQMTVKEWEKVFEMSQDKNLYHNLCTSLFPTIHG
NDEVKRGVLLMLFGGVPKTTGEGTSLRGDINVCIVGDPSTAKSQFLKHVEEFSPRAVYTS
GKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDVRDQVAIHEAMEQQ
TISITKAGVKATLNARTSILAAANPISGHYDRSKSLKQNINLSAPIMSRFDLFFILVDEC
NEVTDYAIARRIVDLHSRIEESIDRVYSLDDIRRYLLFARQFKPKISKESEDFIVEQYKH
LRQRDGSGVTKSSWRITVRQLESMIRLSEAMARMHCCDEVQPKHVKEAFRLLNKSIIRVE
TPDVNLDQEEEIQMEVDEGAGGINGHADSPAPVNGINGYNEDINQESAPKASLRLGFSEY
CRISNLIVLHLRKVEEEEDESALKRSELVNWYLKEIESEIDSEEELINKKRIIEKVIHRL
THYDHVLIELTQAGLKGSTEGSESYEEDPYLVVNPNYLLED

Sequence of entity 6 (7), FASTA

>7PFO_6 DNA replication licensing factor MCM7 (chains 7)
MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDP
ELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVR
SPQNQYPAELMRRFELYFQGPSSNKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVA
TYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEH
SDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEA
HRIVKMNKSEDDESGAGELTREELRQIAEEDFYEKLAASIAPEIYGHEDVKKALLLLLVG
GVDQSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVLR
DSVSGELTLEGGALVLADQGVCCIDEFDKMAEADRTAIHEVMEQQTISIAKAGILTTLNA
RCSILAAANPAYGRYNPRRSLEQNIQLPAALLSRFDLLWLIQDRPDRDNDLRLAQHITYV
HQHSRQPPSQFEPLDMKLMRRYIAMCREKQPMVPESLADYITAAYVEMRREAWASKDATY
TSARTLLAILRLSTALARLRMVDVVEKEDVNEAIRLMEMSKDSLLGDKGQTARTQRPADV
IFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV

Sequence of entity 7 (A), FASTA

>7PFO_7 DNA polymerase epsilon subunit 2 (chains A)
MAPERLRSRALSAFKLRGLLLRGEAIKYLTEALQSISELELEDKLEKIINAVEKQPLSSN
MIERSVVEAAVQECSQSVDETIEHVFNIIGAFDIPRFVYNSERKKFLPLLMTNHPAPNLF
GTPRDKAEMFRERYTILHQRTHRHELFTPPVIGSHPDESGSKFQLKTIETLLGSTTKIGD
AIVLGMITQLKEGKFFLEDPTGTVQLDLSKAQFHSGLYTEACFVLAEGWFEDQVFHVNAF
GFPPTEPSSTTRAYYGNINFFGGPSNTSVKTSAKLKQLEEENKDAMFVFLSDVWLDQVEV
LEKLRIMFAGYSPAPPTCFILCGNFSSAPYGKNQVQALKDSLKTLADIICEYPDIHQSSR
FVFVPGPEDPGFGSILPRPPLAESITNEFRQRVPFSVFTTNPCRIQYCTQEITVFREDLV
NKMCRNCVRFPSSNLAIPNHFVKTILSQGHLTPLPLYVCPVYWAYDYALRVYPVPDLLVI
ADKYDPFTTTNTECLCINPGSFPRSGFSFKVFYPSNKTVEDSKLQGF

Sequence of entity 8 (B), FASTA

>7PFO_8 DNA polymerase epsilon catalytic subunit A (chains B)
MSLRSGGRRRADPGADGEASRDDGATSSVSALKRLERSQWTDKMDLRFGFERLKEPGEKT
GWLINMHPTEILDEDKRLGSAVDYYFIQDDGSRFKVALPYKPYFYIATRKGCEREVSSFL
SKKFQGKIAKVETVPKEDLDLPNHLVGLKRNYIRLSFHTVEDLVKVRKEISPAVKKNREQ
DHASDAYTALLSSVLQRGGVITDEEETSKKIADQLDNIVDMREYDVPYHIRLSIDLKIHV
AHWYNVRYRGNAFPVEITRRDDLVERPDPVVLAFDIETTKLPLKFPDAETDQIMMISYMI
DGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEAHLIQRWFEHVQETKPTIMV
TYNGDFFDWPFVEARAAVHGLSMQQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVG
SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFA
LCTIIPMEPDEVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHVLDSETYV
GGHVEALESGVFRSDIPCRFRMNPAAFDFLLQRVEKTLRHALEEEEKVPVEQVTNFEEVC
DEIKSKLASLKDVPSRIECPLIYHLDVGAMYPNIILTNRLQPSAMVDEATCAACDFNKPG
ANCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFPEGPARAFHELSREEQAKYEK
RRLADYCRKAYKKIHITKVEERLTTICQRENSFYVDTVRAFRDRRYEFKGLHKVWKKKLS
AAVEVGDAAEVKRCKNMEVLYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGA
NIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVFKTTNVKKPKVTISYPGAMLNIM
VKEGFTNDQYQELAEPSSLTYVTRSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFN
EDGSLAELKGFEVKRRGELQLIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSK
AANMPDSELFELISENRSMSRKLEDYGEQKSTSISTAKRLAEFLGDQMVKDAGLSCRYII
SRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQDFDIRAILDWDYYIERLGSA
IQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDVYKQKKISELFTLEGRRQVTMAEA
SEDSPRPSAPDMEDFGLVKLPHPAAPVTVKRKRVLWESQEESQDLTPTVPWQEILGQPPA
LGTSQEEWLVWLRFHKKKWQLQARQRLARRKRQRLESAEGVLRPGAIRDGPATGLGSFLR
RTARSILDLPWQIVQISETSQAGLFRLWALVGSDLHCIRLSIPRVFYVNQRVAKAEEGAS
YRKVNRVLPRSNMVYNLYEYSVPEDMYQEHINEINAELSAPDIEGVYETQVPLLFRALVH
LGCVCVVNKQLVRHLSGWEAETFALEHLEMRSLAQFSYLEPGSIRHIYLYHHAQAHKALF
GIFIPSQRRASVFVLDTVRSNQMPSLGALYSAEHGLLLEKVGPELLPPPKHTFEVRAETD
LKTICRAIQRFLLAYKEERRGPTLIAVQSSWELKRLASEIPVLEEFPLVPICVADKINYG
VLDWQRHGARRMIRHYLNLDTCLSQAFEMSRYFHIPIGNLPEDISTFGSDLFFARHLQRH
NHLLWLSPTARPDLGGKEADDNCLVMEFDDQATVEINSSGCYSTVCVELDLQNLAVNTIL
QSHHVNDMEGADSMGISFDVIQQASLEDMITGGQAASAPASYDETALCSNTFRILKSMVV
GWVKEITQYHNIYADNQVMHFYRWLRSPSSLLHDPALHRTLHNMMKKLFLQLIAEFKRLG
SSVIYANFNRIILCTKKRRVEDAIAYVEYITSSIHSKETFHSLTISFSRCWEFLLWMDPS
NYGGIKGKVSSRIHCGLQDSQKAGGAEDEQENEDDEEERDGEEEEEAEESNVEDLLENNW
NILQFLPQAASCQNYFLMIVSAYIVAVYHCMKDGLRRSAPGSTPVRRRGASQLSQEAEGA
VGALPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNSTELSEMFPVLPGSHLLLNNP
ALEFIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCRSYVLPEVICRS
CNFCRDLDLCKDSSFSEDGAVLPQWLCSNCQAPYDSSAIEMTLVEVLQKKLMAFTLQDLV
CLKCRGVKETSMPVYCSCAGDFALTIHTQVFMEQIGIFRNIAQHYGMSYLLETLEWLLQK
NPQLGH

Sequence of entity 9 (C), FASTA

>7PFO_9 Cell division control protein 45 homolog (chains C)
MFVSDFRKEFYEVVQSQRVLLFVASDVDALCACKILQALFQCDHVQYTLVPVSGWQELET
AFLEHKEQFHYFILINCGANVDLLDILQPDEDTIFFVCDTHRPVNVVNVYNDTQIKLLIK
QDDDLEVPAYEDIFRDEEEDEEHSGNDSDGSEPSEKRTRLDYKDDDIVEQTMRRRQRREW
EARRRDILFDYEQYEYHGTSSAMVMFELAWMLSKDLNDMLWWAIVGLTDQWVQDKITQMK
YVTDVGVLQRHVSRHNHRNEDEENTLSVDCTRISFEYDLRLVLYQHWSLHDSLCNTSYTA
ARFKLWSVHGQKRLQEFLADMGLPLKQVKQKFQAMDISLKENLREMIEESANKFGMKDMR
VQTFSIHFGFKHKFLASDVVFATMSLMESPEKDGSGTDHFIQALDSLSRSNLDKLYHGLE
LAKKQLRATQQTIASCLCTNLVISQGPFLYCSLMEGTPDVMLFSRPASLSLLSKHLLKSF
VCSTKNRRCKLLPLVMAAPLSMEHGTVTVVGIPPETDSSDRKNFFGRAFEKAAESTSSRM
LHNHFDLSVIELKAEDRSKFLDALISLLS

Sequence of entity 10 (D), FASTA

>7PFO_10 DNA replication complex GINS protein PSF1 (chains D)
MFCEKAMELIRELHRAPEGQLPAFNEDGLRQVLEEMKALYEQNQSDVNEAKSGGRSDLIP
TIKFRHCSLLRNRRCTVAYLYDRLLRIRALRWEYGSVLPNALRFHMAAEEMEWFNNYKRS
LATYMRSLGGDEGLDITQDMKPPKSLYIEVRCLKDYGEFEVDDGTSVLLKKNSQHFLPRW
KCEQLIRQGVLEHILS

Sequence of entity 11 (E), FASTA

>7PFO_11 DNA replication complex GINS protein PSF2 (chains E)
MDAAEVEFLAEKELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEVPLWLAINLKQRQKCRL
LPPEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNHASDNIPKADEIRTLVKDM
WDTRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSGTFLTQALNHMYKLRTNLQPLEST
QSQDF

Sequence of entity 12 (F), FASTA

>7PFO_12 DNA replication complex GINS protein PSF3 (chains F)
MSEAYFRVESGALGPEENFLSLDDILMSHEKLPVRTETAMPRLGAFFLERSAGAETDNAV
PQGSKLELPLWLAKGLFDNKRRILSVELPKIYQEGWRTVFSADPNVVDLHKMGPHFYGFG
SQLLHFDSPENADISQSLLQTFIGRFRRIMDSSQNAYNEDTSALVARLDEMERGLFQTGQ
KGLNDFQCWEKGQASQITASNLVQNYKKRKFTDMED

Ligands and cofactors

ID	Name	Formula	Copies
ZN	Zinc ion	Zn	5
MG	Magnesium ion	Mg	3
ANP	Phosphoaminophosphonic acid-adenylate ester	C10 H17 N6 O12 P3	3

Water and common crystallization additives (SO4) are not listed.

Primary citation

Structure of a human replisome shows the organisation and interactions of a DNA replication machine. Jones, M.L., Baris, Y., Taylor, M.R.G. et al. EMBO J (2021) 40:e108819-e108819. DOI 10.15252/embj.2021108819 · PubMed

Other PDB entries of the same protein (UniProt P49736 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6YA7 1.67 Å, Cdc7-Dbf4 bound to an Mcm2-S40 derived bivalent substrate
7CIZ 1.8 Å, Crystal structure of DNAJC9 HBD helix2 in complex with H3.3-H4 dimer and MCM2 HBD
5BNX 2.31 Å, Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.3-H4 dimer
5JA4 2.42 Å, Crystal structure of human TONSL and MCM2 HBDs binding to a histone H3-H4 tetramer
7CJ0 2.5 Å, Crystal structure of DNAJC9 HBD in complex with H3.3-H4 dimer and MCM2 HBD
7W1Y 2.59 Å, Human MCM double hexamer bound to natural DNA duplex (polyAT/polyTA)
9E2Z 2.6 Å, Cryo-EM structure of human CMG helicase stalled at G4-containing DNA template
5BNV 2.79 Å, Crystal structure of Human MCM2 HBD chaperoning a histone H3-H4 tetramer
7PLO 2.8 Å, H. sapiens replisome-CUL2/LRR1 complex
8W0F 2.8 Å, Cryo-EM structure of a human MCM2-7 double hexamer on dsDNA
4UUZ 2.9 Å, MCM2-histone complex
5BO0 2.91 Å, Crystal structure of Human MCM2 HBD and ASF1b chaperoning a histone H3.2-H4 dimer

Browse structure collections

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