7PPN: SHP2 catalytic domain

SHP2 catalytic domain in complex with CD28 (183-198) phosphopeptide (pTyr-191, p-Thr-195). Determined by X-ray diffraction at 1.9 Å resolution. Released 7 Sept 2022.

Method: X-ray diffraction
Resolution: 1.9 Å
Organism: Homo sapiens
Chains: 2
Atoms: 2,400
Mol. weight: 34.92 kDa
Released: 7 Sept 2022

Explore 7PPN in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7PPN contains 11 α-helices and 11 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 11 β-strands

Element	Residues	Length	Sheet
α-helix	6-16	11
α-helix	25-28	4
α-helix	30-35	6
β-strand	48	1	1
β-strand	66-69	4	1
β-strand	81-85	5	1
α-helix	86-88	3
α-helix	92-102	11
β-strand	106-109	4	1
β-strand	114-115	2	2
β-strand	118-119	2	2
α-helix	126-127	2
β-strand	131-134	4	1
β-strand	137-146	10	1
β-strand	150-159	10	1
β-strand	167-174	8	1
α-helix	187-202	16
α-helix	208	1
β-strand	209-213	5	1
α-helix	218-236	19
α-helix	244-252	9
α-helix	262-280	19

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Tyrosine-protein phosphatase non-receptor type 11,Tyrosine-protein phosphatase non-receptor type 11	A	protein	282	Homo sapiens	Q06124 (AlphaFold model)
T-cell-specific surface glycoprotein CD28	B	protein	16	Homo sapiens	P10747 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7PPN_1 Tyrosine-protein phosphatase non-receptor type 11,Tyrosine-protein phosphatase non-receptor type 11 (chains A)
GSGSGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPV
SDYINANIIMPEFGSSGKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSK
CVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDH
GVPSDPGGVLDFLEEVHHKQESIMDAGPVVVHSSAGIGRTGTFIVIDILIDIIREKGVDC
DIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRR

Sequence of entity 2 (B), FASTA

>7PPN_2 T-cell-specific surface glycoprotein CD28 (chains B)
RSRLLHSDYMNMTPRR

Primary citation

Structural insights into the pSer/pThr dependent regulation of the SHP2 tyrosine phosphatase in insulin and CD28 signaling. Zeke, A., Takacs, T., Sok, P. et al. Nat Commun (2022) 13:5439-5439. DOI 10.1038/s41467-022-32918-5 · PubMed

Other PDB entries of the same protein (UniProt Q06124 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9EIK 1.25 Å, Crystal structure of N-SH2 domain of SHP2 with T42A mutation bound to GAB1 tyrosine…
3ZM1 1.4 Å, Catalytic domain of human SHP2
4JMG 1.4 Å, Crystal structure of the synthetic protein in complex with pY peptide
7PPM 1.48 Å, SHP2 catalytic domain in complex with IRS1 (889-901) phosphopeptide (pSer-892, pTyr-896)
3ZM0 1.5 Å, Catalytic domain of human SHP2
3ZM2 1.5 Å, Catalytic domain of human SHP2
3ZM3 1.5 Å, Catalytic domain of human SHP2
7PPL 1.53 Å, SHP2 catalytic domain in complex with IRS1 (625-639) phosphopeptide (pTyr-632, pSer-636)
9EIC 1.58 Å, Crystal structure of unbound N-SH2 domain of SHP2 with T42A mutation
9EHD 1.59 Å, Crystal structure of N-SH2 domain of SHP2 bound to GAB1 tyrosine phosphorylated peptide…
3B7O 1.6 Å, Crystal structure of the human tyrosine phosphatase SHP2 (PTPN11) with an accessible…
4RDD 1.6 Å, Co-crystal structure of SHP2 in complex with a Cefsulodin derivative

Browse structure collections

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