7S6P: Human ISG15

The crystal structure of human ISG15. Determined by X-ray diffraction at 2.15 Å resolution. Released 22 Sept 2021.

Method: X-ray diffraction
Resolution: 2.15 Å
Organism: Homo sapiens
Chains: 6
Atoms: 7,119
Mol. weight: 103.83 kDa
Released: 22 Sept 2021

Explore 7S6P in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7S6P contains 54 α-helices and 72 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chains A and E: 9 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	1
β-strand	14-18	5	1
α-helix	25-36	12
α-helix	40-42	3
β-strand	43-47	5	1
α-helix	52	1
β-strand	53	1	1
α-helix	54-55	2
α-helix	60-63	4
β-strand	70-75	6	1
β-strand	82-87	6	2
β-strand	93-98	6	2
β-strand	103	1	3
α-helix	104-115	12
α-helix	119-121	3
β-strand	122-126	5	2
β-strand	129-130	2	2
α-helix	131-132	2
β-strand	136	1	3
α-helix	137-140	4
β-strand	147-152	6	2

Chain B: 9 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-9	7	4
β-strand	14-19	6	4
α-helix	25-36	12
α-helix	40-42	3
β-strand	43-47	5	4
α-helix	52	1
β-strand	53	1	4
α-helix	54-55	2
α-helix	60-63	4
β-strand	70-75	6	4
β-strand	82-87	6	5
β-strand	93-98	6	5
β-strand	103	1	6
α-helix	104-115	12
α-helix	119-121	3
β-strand	122-126	5	5
β-strand	129-130	2	5
α-helix	131-132	2
β-strand	136	1	6
α-helix	137-140	4
β-strand	147-152	6	5

Chains C and F: 9 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	7
β-strand	14-18	5	7
α-helix	25-36	12
α-helix	40-42	3
β-strand	43-48	6	7
α-helix	52	1
β-strand	53	1	7
α-helix	54-55	2
α-helix	60-63	4
β-strand	70-75	6	7
β-strand	82-87	6	8
β-strand	93-98	6	8
β-strand	103	1	9
α-helix	104-115	12
α-helix	119-121	3
β-strand	122-126	5	8
β-strand	129-130	2	8
α-helix	131-132	2
β-strand	136	1	9
α-helix	137-140	4
β-strand	147-152	6	8

Chain D: 9 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	4-9	6	10
β-strand	14-18	5	10
α-helix	25-36	12
α-helix	40-42	3
β-strand	43-47	5	10
α-helix	52	1
β-strand	53	1	10
α-helix	54-55	2
α-helix	60-63	4
β-strand	70-75	6	10
β-strand	82-87	6	11
β-strand	93-98	6	11
β-strand	103	1	12
α-helix	104-115	12
α-helix	119-121	3
β-strand	123-126	4	11
β-strand	129-130	2	11
α-helix	131-132	2
β-strand	136	1	12
α-helix	137-140	4
β-strand	147-151	5	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Ubiquitin-like protein ISG15	A, B, C, D, E, F	protein	159	Homo sapiens	P05161 (AlphaFold model)

Sequence of entity 1 (A, B, C, D, E, F), FASTA

>7S6P_1 Ubiquitin-like protein ISG15 (chains A, B, C, D, E, F)
SNAGWDLTVKMLAGNEFQVSLSSSMSVSELKAQITQKIGVHAFQQRLAVHPSGVALQDRV
PLASQGLGPGSTVLLVVDKCDEPLSILVRNNKGRSSTYEVRLTQTVAHLKQQVSGLEGVQ
DDLFWLTFEGKPLEDQLPLGEYGLKPLSTVFMNLRLRGG

Primary citation

Dual domain recognition determines SARS-CoV-2 PLpro selectivity for human ISG15 and K48-linked di-ubiquitin. Wydorski, P.M., Osipiuk, J., Lanham, B.T. et al. Nat Commun (2023) 14:2366-2366. DOI 10.1038/s41467-023-38031-5 · PubMed

Other PDB entries of the same protein (UniProt P05161 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6FFA 1.5 Å, FMDV Leader protease bound to substrate ISG15
3PHX 1.6 Å, OTU Domain of Crimean Congo Hemorrhagic Fever Virus in complex with ISG15
3RT3 2.01 Å, Complex of influenza virus protein with host anti-viral factor
3SDL 2.29 Å, Crystal structure of human ISG15 in complex with NS1 N-terminal region from influenza B…
6BI8 2.29 Å, X-ray structure of MERS coronavirus papain-like protease in complex with human ISG15
3PSE 2.3 Å, Structure of a viral OTU domain protease bound to interferon-stimulated gene 15 (ISG15)
3R66 2.3 Å, Crystal structure of human ISG15 in complex with NS1 N-terminal region from influenza…
5W8U 2.41 Å, Crystal structure of MERS-CoV papain-like protease in complex with the C-terminal domain…
1Z2M 2.5 Å, Crystal Structure of ISG15, the Interferon-Induced Ubiquitin Cross Reactive Protein
9NN9 2.59 Å, ISG15 complexed with nanobody
5TL6 2.62 Å, Crystal structure of SARS-CoV papain-like protease in complex with the C-terminal domain…
9LW4 2.7 Å, UbCh8-ISG15 complex

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