7SDE: Nse5/6 heterodimer

Cryo-EM structure of Nse5/6 heterodimer. Determined by electron microscopy at 3.2 Å resolution. Released 13 Oct 2021.

Method
Electron microscopy
Resolution
3.2 Å
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Chains
2
Atoms
5,734
Mol. weight
131.63 kDa
Released
13 Oct 2021

Explore 7SDE in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7SDE contains 42 α-helices and 6 β-strands across 2 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 28 helices, 3 β-strands

ElementResiduesLengthSheet
α-helix26-4015
α-helix44-529
α-helix63-653
α-helix66-7510
α-helix84-907
α-helix100-11516
α-helix127-13913
β-strand19811
β-strand20111
α-helix205-2084
α-helix216-2194
α-helix230-2389
α-helix240-26021
α-helix269-2757
α-helix280-2856
α-helix286-2883
α-helix293-3019
α-helix318-3203
β-strand33612
α-helix340-36223
α-helix369-3713
α-helix381-39010
α-helix395-4028
α-helix414-42512
α-helix438-4414
α-helix445-4495
α-helix452-4565
α-helix472-49524
α-helix506-51712
α-helix519-52810
α-helix540-5467
Chain B: 14 helices, 3 β-strands
ElementResiduesLengthSheet
α-helix209-2168
α-helix220-2245
α-helix246-2527
β-strand28112
α-helix284-2918
α-helix301-31616
α-helix318-3214
α-helix330-3356
α-helix340-3456
α-helix355-36511
α-helix371-3777
α-helix389-3968
α-helix405-41511
β-strand41613
β-strand42113
α-helix433-4408
α-helix449-46113

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Non-structural maintenance of chromosome element 5Aprotein555Saccharomyces cerevisiae (strain ATCC 204508 / S288c)Q03718 (AlphaFold model)
Ubiquitin-like protein SMT3,DNA repair protein KRE29 chimeraBprotein586Saccharomyces cerevisiae (strain ATCC 204508 / S288c)P40026 (AlphaFold model), Q12306 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>7SDE_1 Non-structural maintenance of chromosome element 5 (chains A)
MDGALINSVLYVSPRNGAHYFVELTEKHLLAFEMLNSMCLLENYDHVLLFLECQFGKSHN
LAVIPFDIILVLFTLSTLSEYYKEPILRANDPYNTSRETLSRRALKLLQKYLAILKEFDS
EQYNLYDLELLRCQFFLAIDTLTPKKQKWGFDRFRRTKSESGVTYRQNASVDPELDQAKT
FKNPYRSYISCLEQRNTILGNRLLNLKLNEPGEFINMILWTLSNSLQESTPLFLSSHEIW
MPLLEILIDLFSCRQDYFIQHEAQNVSKSLFVQRLSESPLAVFFESLNTRNFANRFSEYV
FLNCDYKLPSDNYATPVHPVYNGENTIVDTYIPTIKCSPLYKSQKSLALRRKLIGSCFKL
LLRVPDGHRLITPRIVADDVIQGISRTLASFNDILQFKKFFMTENLSQESYFIPLLAEGT
LSEILKDTQECVVILTLVENLSDGVSFCNEVIGLVKSKCFAFTEQCSQASYEEAVLNIEK
CDVCLLVLLRYLLHLIGTEAILDAKEQLEMLHAIEKNDSGRRQWAKALNLGNDPPLLYPI
VSQMFGVHDKSVIIE
Sequence of entity 2 (B), FASTA
>7SDE_2 Ubiquitin-like protein SMT3,DNA repair protein KRE29 chimera (chains B)
MGSSHHHHHHSSGLVPRGSHMASMSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFF
KIKKTTPLRRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIG
GSMGSVNSSPNEEFETVPDSQISGFDSPLIPTSVGSYFRDDDDDEKVHPNFISDPENDSL
NSDEEFSSLENSDLNLSGAKAESGDDFDPILKRTIISKRKAPSNNEDEEIVKTPRKLVNY
VPLKIFNLGDSFDDTITTTVAKLQDLKKEILDSPRSNKSIVITSNTVAKSELQKSIKFSG
SIPEIYLDVVTKETISDKYKDWHFISKNCHYEQLMDLEMKDTAYSFLFGSSRSQGKVPEF
VHLKCPSITNLLVLFGVNQEKCNSLKINYEKKENSRYDNLCTIFPVNKMLKFLMYFYSDD
DNDDVREFFLKAFICLILDRKVFNAMESDHRLCFKVLELFNEAHFINSYFEIVDKNDFFL
HYRLLQIFPHLQSALLRRRFSEKQGRTETIQQNIIKEFNEFFDCKNYKNLLYFILTMYGS
KFIPFGPKCQVTEYFKDCILDISNETTNDVEISILKGILNLFSKIR

Primary citation

The cryo-EM structure of Nse5/6 complex with the C terminal part of Nse5. Yu, Y., Patel, D.J. To be published.

Other PDB entries of the same protein (UniProt Q03718 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

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