7SF8: GPR56 (ADGRG1) 7TM domain

GPR56 (ADGRG1) 7TM domain bound to tethered agonist in complex with G protein heterotrimer. Determined by electron microscopy at 2.7 Å resolution. Released 27 Apr 2022.

Method: Electron microscopy
Resolution: 2.7 Å
Organism: Homo sapiens
Chains: 4
Atoms: 6,661
Mol. weight: 106.65 kDa
Released: 27 Apr 2022

Explore 7SF8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7SF8 contains 27 α-helices and 36 β-strands across 4 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 13 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	385-387	3
α-helix	397-426	30
α-helix	435-463	29
α-helix	468-498	31
α-helix	509-528	20
α-helix	533-535	3
β-strand	536-537	2	1
β-strand	557-558	2	1
α-helix	561-564	4
α-helix	565-569	5
α-helix	570-590	21
α-helix	603-613	11
α-helix	615-624	10
α-helix	630-642	13
α-helix	644-655	12

Chain B: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	13-25	13
α-helix	27-31	5
β-strand	34-39	6	2
α-helix	46-51	6
β-strand	69-75	7	2
β-strand	80-86	7	2
β-strand	105-110	6	2
α-helix	118-129	12
β-strand	138-143	6	2
α-helix	149-152	4
α-helix	158-160	3
α-helix	172-184	13
β-strand	195-199	5	2
α-helix	207-227	21

Chain C: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-24	21
α-helix	30-33	4
α-helix	38-39	2
β-strand	47-51	5	3
β-strand	58-63	6	4
β-strand	69-74	6	4
β-strand	78-83	6	4
β-strand	88-94	7	4
β-strand	100-105	6	5
β-strand	111-116	6	5
β-strand	121-125	5	5
β-strand	134-139	6	5
β-strand	146-151	6	6
β-strand	156-161	6	6
β-strand	166-170	5	6
β-strand	175-180	6	6
β-strand	187-192	6	7
β-strand	198-203	6	7
β-strand	207-212	6	7
β-strand	218-223	6	7
β-strand	229-234	6	8
β-strand	240-245	6	8
β-strand	250-254	5	8
β-strand	259-264	6	8
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	303-308	6	9
β-strand	315-320	6	3
β-strand	327-331	5	3
β-strand	336-339	4	3

Chain D: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-23	14
α-helix	30-43	14
α-helix	53-55	3

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 2 of Adhesion G-protein coupled receptor G1	A	protein	313	Homo sapiens	Q9Y653 (AlphaFold model)
G protein subunit 13 (Gi2-mini-G13 chimera)	B	protein	230	Homo sapiens	Q14344 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	C	protein	340	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	D	protein	71	Homo sapiens	P59768 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7SF8_1 Isoform 2 of Adhesion G-protein coupled receptor G1 (chains A)
TYFAVLMVSSVEVDAVHKHYLSLLSYVGCVVSALACLVTIAAYLCSRRKPRDYTIKVHMN
LLLAVFLLDTSFLLSEPVALTGSEAGCRASAIFLHFSLLTCLSWMGLEGYNLYRLVVEVF
GTYVPGYLLKLSAMGWGFPIFLVTLVALVDVDNYGPIILAVHRTPEGVIYPSMCWIRDSL
VSYITNLGLFSLVFLFNMAMLATMVVQILRLRPHTQKWSHVLTLLGLSLVLGLPWALIFF
SFASGTFQLVVLYLFSIITSFQGFLIFIWYWSMRLQARGGPSPLKSNSDSARLPISSGST
SSSRIGSLEVLFQ

Sequence of entity 2 (B), FASTA

>7SF8_2 G protein subunit 13 (Gi2-mini-G13 chimera) (chains B)
MGSTVSAEDKAAAERSKEIDKCLSREKTYVKRLVKILLLGADNSGKSTFLKQMRIIHGGS
GGSGGTKGIHEYDFEIKNVPFKMVDVGGQRSERKRWFECFDSVTSILFLVDSSDFNRLTE
SLNDFETIVNNRVFSNVSIILFLNKTDLLEEKVQIVSIKDYFLEFEGDPHCLRDVQKFLV
ECFRNKRRDQQQKPLYHHFTTAINTENARLIFRDVKDTILHDNLKQLMLQ

Sequence of entity 3 (C), FASTA

>7SF8_3 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains C)
MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYA
MHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNI
CSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTF
TGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNA
FATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDAL
KADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 4 (D), FASTA

>7SF8_4 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains D)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Primary citation

The tethered peptide activation mechanism of adhesion GPCRs. Barros-Alvarez, X., Nwokonko, R.M., Vizurraga, A. et al. Nature (2022) 604:757-762. DOI 10.1038/s41586-022-04575-7 · PubMed

Other PDB entries of the same protein (UniProt Q9Y653 (AlphaFold model), which also has an AlphaFold model), best resolution first:

9S9U 1.92 Å, Structure of the human ADGRG1 GAIN domain with engineered disulfides

Browse structure collections

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