7SL6: Full-length insulin receptor

Full-length insulin receptor bound with site 2 binding deficient mutant insulin (B-L17R) -- symmetric conformation. Determined by electron microscopy at 3.7 Å resolution. Released 30 Mar 2022.

Method: Electron microscopy
Resolution: 3.7 Å
Organisms: Mus musculus, Homo sapiens
Chains: 6
Atoms: 14,004
Mol. weight: 323.3 kDa
Released: 30 Mar 2022

Explore 7SL6 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7SL6 contains 64 α-helices and 150 β-strands across 6 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 29 helices, 74 β-strands

Element	Residues	Length	Sheet
β-strand	5-6	2	1
β-strand	12-14	3	2
α-helix	18-23	6
β-strand	26	1	1
β-strand	28-29	2	3
β-strand	33-38	6	2
α-helix	44-47	4
β-strand	56-57	2	3
β-strand	61-66	6	2
β-strand	71	1	4
β-strand	81-82	2	3
β-strand	93-96	4	2
β-strand	103	1	4
β-strand	111-112	2	3
β-strand	117-122	6	2
α-helix	133-135	3
α-helix	140-142	3
β-strand	144-148	5	2
α-helix	149-152	4
β-strand	171-173	3	5
β-strand	178-180	3	5
β-strand	182	1	6
β-strand	188	1	6
α-helix	189-190	2
β-strand	201	1	7
β-strand	207	1	7
β-strand	212	1	8
β-strand	216	1	9
α-helix	222-224	3
β-strand	225	1	9
β-strand	228	1	8
β-strand	232-233	2	10
β-strand	236-237	2	10
α-helix	241-242	2
β-strand	246-248	3	10
β-strand	252-254	3	10
α-helix	256-267	12
β-strand	278-280	3	10
β-strand	283-285	3	10
β-strand	292-294	3	11
β-strand	300	1	10
β-strand	301-303	3	11
α-helix	304	1
β-strand	311-313	3	12
α-helix	315-317	3
β-strand	319-321	3	13
α-helix	324-328	5
β-strand	335-337	3	12
β-strand	340-342	3	13
α-helix	351-357	7
β-strand	363-364	2	12
β-strand	368-371	4	13
β-strand	378	1	14
α-helix	379-381	3
β-strand	387-388	2	12
β-strand	394	1	13
β-strand	398-403	6	13
β-strand	410	1	14
β-strand	420-421	2	12
β-strand	425-429	5	13
α-helix	436-446	11
α-helix	449-451	3
β-strand	457	1	13
β-strand	472-473	2	15
β-strand	475	1	16
β-strand	479-480	2	17
β-strand	485-487	3	17
β-strand	489	1	16
α-helix	497-499	3
β-strand	500-509	10	15
β-strand	530-534	5	15
α-helix	535-538	4
β-strand	553-554	2	17
α-helix	558-559	2
β-strand	563-572	10	15
β-strand	584-585	2	15
β-strand	588-591	4	15
α-helix	592-594	3
α-helix	598-600	3
β-strand	601-607	7	18
β-strand	613-618	6	18
β-strand	629-633	5	19
β-strand	636	1	19
α-helix	637-639	3
α-helix	641-644	4
α-helix	691-715	25
β-strand	761-764	4	19
β-strand	769-772	4	18
β-strand	781-788	8	19
α-helix	799-800	2
β-strand	801-805	5	19
α-helix	807-809	3
β-strand	821-824	4	20
β-strand	830-833	4	20
β-strand	844-853	10	21
β-strand	858-864	7	21
α-helix	865-871	7
β-strand	874-876	3	20
α-helix	879-880	2
β-strand	883-892	10	21
β-strand	896	1	21
α-helix	897-902	6
β-strand	903-906	4	21

Chain B: 27 helices, 72 β-strands

Element	Residues	Length	Sheet
β-strand	5-6	2	22
β-strand	12-14	3	23
α-helix	18-23	6
β-strand	26	1	22
β-strand	28-29	2	24
β-strand	33-38	6	23
α-helix	44-47	4
β-strand	56-57	2	24
β-strand	61-66	6	23
β-strand	81-82	2	24
β-strand	93-96	4	23
β-strand	111-112	2	24
β-strand	117-122	6	23
α-helix	133-135	3
α-helix	140-142	3
β-strand	144-148	5	23
α-helix	149-152	4
β-strand	171-173	3	25
β-strand	178-180	3	25
β-strand	182-184	3	26
β-strand	187-188	2	26
α-helix	189-190	2
β-strand	201	1	27
β-strand	207	1	27
β-strand	212	1	28
β-strand	216	1	29
α-helix	222-224	3
β-strand	225	1	29
β-strand	228	1	28
β-strand	231-233	3	30
β-strand	236-238	3	30
α-helix	241-242	2
β-strand	246-248	3	30
β-strand	252-254	3	30
α-helix	256-267	12
β-strand	278-280	3	30
β-strand	283-285	3	30
β-strand	292-294	3	31
β-strand	300	1	30
β-strand	301-303	3	31
α-helix	304	1
β-strand	311-313	3	32
α-helix	315-317	3
β-strand	319-321	3	33
α-helix	324-328	5
β-strand	335-337	3	32
β-strand	340-342	3	33
α-helix	351-357	7
β-strand	363-364	2	32
β-strand	368-371	4	33
β-strand	378	1	34
α-helix	379-381	3
β-strand	387-388	2	32
β-strand	394	1	33
β-strand	398-403	6	33
β-strand	410	1	34
β-strand	420-421	2	32
β-strand	425-429	5	33
α-helix	436-446	11
α-helix	449-451	3
β-strand	457	1	33
β-strand	472-473	2	35
β-strand	475	1	36
β-strand	479-480	2	37
β-strand	485-487	3	37
β-strand	489	1	36
α-helix	497-499	3
β-strand	500-509	10	35
β-strand	530-534	5	35
α-helix	535-537	3
β-strand	553-554	2	37
α-helix	558-559	2
β-strand	563-572	10	35
β-strand	584-585	2	35
β-strand	588-591	4	35
α-helix	598-600	3
β-strand	601-606	6	38
β-strand	613-618	6	38
β-strand	629-633	5	39
β-strand	636	1	39
α-helix	641-644	4
α-helix	691-715	25
β-strand	761-764	4	39
β-strand	769-772	4	38
β-strand	780-788	9	39
α-helix	799-800	2
β-strand	801-806	6	39
α-helix	807-810	4
β-strand	821-824	4	40
β-strand	830-833	4	40
β-strand	844-853	10	41
β-strand	858-864	7	41
α-helix	865-871	7
β-strand	874-876	3	40
α-helix	879-880	2
β-strand	883-892	10	41
β-strand	896	1	41
α-helix	897-902	6
β-strand	903-906	4	41

Chains C and D: 3 helices, 1 β-strand

Element	Residues	Length	Sheet
α-helix	2-3	2
α-helix	4-8	5
α-helix	15-19	5
β-strand	20	1	42

Chains E and F: 1 helix, 1 β-strand

Element	Residues	Length	Sheet
α-helix	9-18	10
β-strand	24	1	42

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Insulin receptor	A, B	protein	1372	Mus musculus	P15208 (AlphaFold model)
Insulin B chain	E, F	protein	30	Homo sapiens	P01308 (AlphaFold model)
Insulin A chain	C, D	protein	21	Homo sapiens	P01308 (AlphaFold model)

Sequence of entity 1 (A, B), FASTA

>7SL6_1 Insulin receptor (chains A, B)
MGFGRGCETTAVPLLVAVAALLVGTAGHLYPGEVCPGMDIRNNLTRLHELENCSVIEGHL
QILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLTVIRGSRLFFNYAL
VIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNYIVLNKDDNE
ECGDVCPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHKECL
GNCSEPDDPTKCVACRNFYLDGQCVETCPPPYYHFQDWRCVNFSFCQDLHFKCRNSRKPG
CHQYVIHNNKCIPECPSGYTMNSSNLMCTPCLGPCPKVCQILEGEKTIDSVTSAQELRGC
TVINGSLIINIRGGNNLAAELEANLGLIEEISGFLKIRRSYALVSLSFFRKLHLIRGETL
EIGNYSFYALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQE
RNDIALKTNGDQASCENELLKFSFIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQ
NVTEFDGQDACGSNSWTVVDIDPPQRSNDPKSQTPSHPGWLMRGLKPWTQYAIFVKTLVT
FSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVY
WERQAEDSELFELDYCLKGLKLPSRTWSPPFESDDSQKHNQSEYDDSASECCSCPKTDSQ
ILKELEESSFRKTFEDYLHNVVFVPRPSRKRRSLEEVGNVTATTLTLPDFPNVSSTIVPT
SQEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDSPDERCSVAAYVSARTMPEAKA
DDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERG
CRLRGLSPGNYSVRVRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSV
VIGSIYLFLRKRQPDGPMGPLYASSNPEYLSASDVFPSSVYVPDEWEVPREKITLLRELG
QGSFGMVYEGNAKDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLL
GVVSKGQPTLVVMELMAHGDLKSHLRSLRPDAENNPGRPPPTLQEMIQMTAEIADGMAYL
NAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKD
GVFTASSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDPPDNCPERLTDLMR
MCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFYSEENKAPESEELEMEFEDMENVPLD
RSSHCQREEAGGREGGSSLSIKRTYDEHIPYTHMNGGKKNGRVLTLPRSNPS

Sequence of entity 2 (E, F), FASTA

>7SL6_2 Insulin B chain (chains E, F)
FVNQHLCGSHLVEALYRVCGERGFFYTPKT

Sequence of entity 3 (C, D), FASTA

>7SL6_3 Insulin A chain (chains C, D)
GIVEQCCTSICSLYQLENYCN

Primary citation

Synergistic activation of the insulin receptor via two distinct sites. Li, J., Park, J., Mayer, J.P. et al. Nat Struct Mol Biol (2022) 29:357-368. DOI 10.1038/s41594-022-00750-6 · PubMed

Other PDB entries of the same protein (UniProt P15208 (AlphaFold model), which also has an AlphaFold model), best resolution first:

1LK2 1.35 Å, 1.35A crystal structure of H-2Kb complexed with the GNYSFYAL peptide
7SL7 3.1 Å, Full-length insulin receptor bound with both site 1 binding deficient mutant insulin…
7SL1 3.4 Å, Full-length insulin receptor bound with site 1 binding deficient mutant insulin (A-V3E)
7SL3 3.4 Å, Full-length insulin receptor bound with site 2 binding deficient mutant insulin (A-L13R)…
7STH 3.5 Å, Full-length insulin receptor bound with unsaturated insulin WT (2 insulin bound)…
8DTM 3.5 Å, Cryo-EM structure of insulin receptor (IR) bound with S597 component 2
7SL2 3.6 Å, Full-length insulin receptor bound with site 2 binding deficient mutant insulin (A-L13R)…
8EZ0 3.7 Å, Cryo-EM structure of 4 insulins bound full-length mouse IR mutant with physically…
7STK 4.0 Å, Full-length insulin receptor bound with unsaturated insulin WT (2 insulins bound)…
7STJ 4.4 Å, Full-length insulin receptor bound with unsaturated insulin WT (2 insulins bound)…
8EYX 4.5 Å, Cryo-EM structure of 4 insulins bound full-length mouse IR mutant with physically…
7STI 4.9 Å, Full-length insulin receptor bound with unsaturated insulin WT (1 insulin bound)…

Browse structure collections

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