7SMK: Ribulose bisphosphate carboxylase large chain

H. neapolitanus carboxysomal rubisco/CsoSCA-peptide (1-50)complex. Determined by electron microscopy at 1.98 Å resolution. Released 24 May 2023.

Method
Electron microscopy
Resolution
1.98 Å
Organism
Halothiobacillus neapolitanus (strain ATCC 23641 / c2)
Chains
3
Atoms
4,729
Mol. weight
72.39 kDa
Released
24 May 2023

Explore 7SMK in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7SMK contains 32 α-helices and 28 β-strands across 3 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 25 helices, 24 β-strands

ElementResiduesLengthSheet
β-strand911
α-helix14-174
β-strand1812
β-strand29-3792
α-helix43-5412
β-strand6111
α-helix63-675
α-helix70-734
β-strand76-8382
β-strand86-96112
α-helix98-1003
α-helix106-1149
α-helix117-1193
β-strand123-132102
α-helix135-1384
α-helix148-1558
β-strand162-16433
β-strand16614
α-helix175-18713
β-strand192-19434
β-strand20215
β-strand20515
α-helix207-22519
β-strand230-23234
β-strand233-23423
α-helix240-25213
β-strand257-26153
α-helix262-2654
α-helix267-28014
β-strand283-28753
α-helix291-2955
β-strand301-30222
α-helix304-31411
β-strand318-32033
α-helix329-34315
β-strand346-34726
β-strand35017
α-helix351-3533
β-strand35517
β-strand359-36026
α-helix364-3663
β-strand368-37143
α-helix377-3793
α-helix380-3878
β-strand391-39443
α-helix397-4004
α-helix406-42520
α-helix434-4429
α-helix446-4549
Chain B: 6 helices, 4 β-strands
ElementResiduesLengthSheet
α-helix6-94
α-helix20-223
α-helix24-3613
β-strand40-4678
α-helix48-503
α-helix561
β-strand57-5828
α-helix69-8214
β-strand87-9488
β-strand99-10798
Chain C: 1 helix, 0 β-strands
ElementResiduesLengthSheet
α-helix23-264

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Ribulose bisphosphate carboxylase large chainAprotein482Halothiobacillus neapolitanus (strain ATCC 23641 / c2)O85040 (AlphaFold model)
Ribulose bisphosphate carboxylase small chainBprotein110Halothiobacillus neapolitanus (strain ATCC 23641 / c2)P45686 (AlphaFold model)
Carboxysome shell carbonic anhydraseCprotein50Halothiobacillus neapolitanus (strain ATCC 23641 / c2)O85042 (AlphaFold model)
Sequence of entity 1 (A), FASTA
>7SMK_1 Ribulose bisphosphate carboxylase large chain (chains A)
MSAVKKYSAGVKEYRQTYWMPEYTPLDSDILACFKITPQPGVDREEAAAAVAAESSTGTW
TTVWTDLLTDMDYYKGRAYRIEDVPGDDAAFYAFIAYPIDLFEEGSVVNVFTSLVGNVFG
FKAVRGLRLEDVRFPLAYVKTCGGPPHGIQVERDKMNKYGRPLLGCTIKPKLGLSAKNYG
RAVYECLRGGLDFTKDDENINSQPFMRWRDRFLFVQDATETAEAQTGERKGHYLNVTAPT
PEEMYKRAEFAKEIGAPIIMHDYITGGFTANTGLAKWCQDNGVLLHIHRAMHAVIDRNPN
HGIHFRVLTKILRLSGGDHLHTGTVVGKLEGDRASTLGWIDLLRESFIPEDRSRGIFFDQ
DWGSMPGVFAVASGGIHVWHMPALVNIFGDDSVLQFGGGTLGHPWGNAAGAAANRVALEA
CVEARNQGRDIEKEGKEILTAAAQHSPELKIAMETWKEIKFEFDTVDKLDTQNRWSHPQF
EK
Sequence of entity 2 (B), FASTA
>7SMK_2 Ribulose bisphosphate carboxylase small chain (chains B)
MAEMQDYKQSLKYETFSYLPPMNAERIRAQIKYAIAQGWSPGIEHVEVKNSMNQYWYMWK
LPFFGEQNVDNVLAEIEACRSAYPTHQVKLVAYDNYAQSLGLAFVVYRGN
Sequence of entity 3 (C), FASTA
>7SMK_3 Carboxysome shell carbonic anhydrase (chains C)
MNTRNTRSKQRAPFGVSSSVKPRLDLIEQAPNPAYDRHPACITLPERTCR

Primary citation

Identification of a carbonic anhydrase-Rubisco complex within the alpha-carboxysome. Blikstad, C., Dugan, E.J., Laughlin, T.G. et al. Proc Natl Acad Sci U S A (2023) 120:e2308600120-e2308600120. DOI 10.1073/pnas.2308600120 · PubMed

Other PDB entries of the same protein (UniProt O85040 (AlphaFold model), which also has an AlphaFold model), best resolution first:

Browse structure collections

About this viewer

MolViewer shows 7SMK directly in your browser with nothing to install. Switch between cartoon, ball-and-stick, spacefill and surface views, color by chain, secondary structure or B-factor, measure distances, angles and dihedrals, and share or embed the view.