7T2H: Mu-opioid receptor - Gi protein complex

CryoEM structure of mu-opioid receptor - Gi protein complex bound to lofentanil (LFT). Determined by electron microscopy at 3.2 Å resolution. Released 7 Dec 2022.

Method: Electron microscopy
Resolution: 3.2 Å
Organisms: Homo sapiens, Mus musculus
Chains: 5
Atoms: 8,677
Mol. weight: 154.14 kDa
Ligands: EID
Released: 7 Dec 2022

Explore 7T2H in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7T2H contains 36 α-helices and 65 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 8 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	9-29	21
β-strand	33-39	7	1
α-helix	46-52	7
β-strand	185-190	6	1
β-strand	195-200	6	1
α-helix	208-210	3
β-strand	220-226	7	1
α-helix	227-229	3
α-helix	242-252	11
β-strand	263-269	7	1
α-helix	271-278	8
α-helix	296-308	13
β-strand	319-322	4	1
α-helix	331-350	20

Chain B: 5 helices, 29 β-strands

Element	Residues	Length	Sheet
α-helix	8-24	17
α-helix	30-33	4
α-helix	34-36	3
β-strand	47-51	5	2
β-strand	58-63	6	3
β-strand	69-74	6	3
β-strand	78-83	6	3
β-strand	88-94	7	3
β-strand	100-105	6	4
β-strand	111-116	6	4
β-strand	121-125	5	4
α-helix	129-131	3
β-strand	134-139	6	4
β-strand	146-151	6	5
β-strand	156-161	6	5
β-strand	165-170	6	5
β-strand	178-180	3	5
β-strand	191-192	2	6
β-strand	198-201	4	6
β-strand	207-212	6	6
β-strand	220-223	4	6
β-strand	229-234	6	7
β-strand	240-245	6	7
β-strand	249-252	4	8
β-strand	253-254	2	7
β-strand	262-265	4	8
α-helix	272	1
β-strand	273-278	6	9
β-strand	284-289	6	9
β-strand	294-298	5	9
β-strand	304-308	5	9
β-strand	315-320	6	2
β-strand	327-331	5	2
β-strand	336-339	4	2

Chain C: 3 helices, 0 β-strands

Element	Residues	Length
α-helix	10-21	12
α-helix	30-43	14
α-helix	53-55	3

Chain D: 17 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	67-92	26
α-helix	93-97	5
α-helix	102-118	17
α-helix	121-130	10
α-helix	138-170	33
α-helix	172-178	7
α-helix	181-204	24
β-strand	206-210	5	18
β-strand	215-219	5	18
α-helix	225-227	3
α-helix	229-237	9
α-helix	238-242	5
α-helix	243-259	17
α-helix	263-265	3
α-helix	269-305	37
α-helix	308-310	3
α-helix	312-333	22
α-helix	334-338	5
α-helix	341-343	3

Chain E: 3 helices, 28 β-strands

Element	Residues	Length	Sheet
β-strand	4-7	4	10
β-strand	11-12	2	11
β-strand	17-24	8	10
α-helix	29-31	3
β-strand	33-39	7	12
β-strand	45-51	7	12
β-strand	58-60	3	12
β-strand	65	1	10
β-strand	68-73	6	10
β-strand	78-84	7	10
β-strand	92	1	13
β-strand	93-99	7	12
β-strand	111	1	12
β-strand	117	1	13
β-strand	118-119	2	11
β-strand	129	1	14
β-strand	134-136	3	15
β-strand	143-147	5	16
β-strand	148	1	14
β-strand	154	1	17
β-strand	160	1	17
β-strand	162-167	6	15
β-strand	174-178	5	15
β-strand	182-183	2	15
α-helix	184	1
β-strand	191-196	6	16
β-strand	199-204	6	16
β-strand	213-219	7	15
α-helix	225	1
β-strand	226	1	15
β-strand	231-234	4	15

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Guanine nucleotide-binding protein G(i) subunit alpha-1	A	protein	354	Homo sapiens	P63096 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	344	Homo sapiens	P62873 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
scFv16	E	protein	259	Mus musculus
Mu-type opioid receptor	D	protein	356	Mus musculus	P42866 (AlphaFold model)

Sequence of entity 1 (A), FASTA

>7T2H_1 Guanine nucleotide-binding protein G(i) subunit alpha-1 (chains A)
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEM
NRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAA
AYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF

Sequence of entity 2 (B), FASTA

>7T2H_2 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
PGSSGSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLA
KIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGG
LDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQ
TTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFP
NGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNV
WDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Sequence of entity 3 (C), FASTA

>7T2H_3 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 4 (E), FASTA

>7T2H_4 scFv16 (chains E)
DVQLVESGGGLVQPGGSRKLSCSASGFAFSSFGMHWVRQAPEKGLEWVAYISSGSGTIYY
ADTVKGRFTISRDDPKNTLFLQMTSLRSEDTAMYYCVRSIYYYGSSPFDFWGQGTTLTVS
SGGGGSGGGGSGGGGSDIVMTQATSSVPVTPGESVSISCRSSKSLLHSNGNTYLYWFLQR
PGQSPQLLIYRMSNLASGVPDRFSGSGSGTAFTLTISRLEAEDVGVYYCMQHLEYPLTFG
AGTKLELKAAAHHHHHHHH

Sequence of entity 5 (D), FASTA

>7T2H_5 Mu-type opioid receptor (chains D)
NISDCSDPLAPASCSPAPGSWLNLSHVDGNQSDPCGPNRTGLGENLYFQGSHSLCPQTGS
PSMVTAITIMALYSIVCVVGLFGNFLVMYVIVRYTKMKTATNIYIFNLALADALATSTLP
FQSVNYLMGTWPFGNILCKIVISIDYYNMFTSIFTLCTMSVDRYIAVCHPVKALDFRTPR
NAKIVNVCNWILSSAIGLPVMFMATTKYRQGSIDCTLTFSHPTWYWENLLKICVFIFAFI
MPVLIITVCYGLMILRLKSVRMLSGSKEKDRNLRRITRMVLVVVAVFIVCWTPIHIYVII
KALITIPETTFQTVSWHFCIALGYTNSCLNPVLYAFLDENFKRCFREFCIPTSSTI

Ligands and cofactors

ID	Name	Formula	Copies
EID	Lofentanil	C25 H32 N2 O3	1

Primary citation

Insights into distinct signaling profiles of the mu OR activated by diverse agonists. Qu, Q., Huang, W., Aydin, D. et al. Nat Chem Biol (2022). DOI 10.1038/s41589-022-01208-y · PubMed

Other PDB entries of the same protein (UniProt P63096 (AlphaFold model), which also has an AlphaFold model), best resolution first:

6CRK 2.0 Å, Heterotrimeric G-protein in complex with an antibody fragment
3UMR 2.04 Å, Crystal structure of the G202D mutant of human G-alpha-i1
9P7Z 2.1 Å, NTSR1-Gi-NTS(8-13) Complex in the Canonical, AHD Open State (C-Open-Apo)
2OM2 2.2 Å, Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14
8YN9 2.3 Å, Cryo-EM structure of histamine H4 receptor in complex with histamine and Gi
9HYI 2.3 Å, DP81-bound serotonin 5-HT1A receptor - Gi Protein Complex
9O36 2.3 Å, CryoEM structure of mu-opioid receptor - Gi protein complex bound to fluornitrazene (FNZ)
9P80 2.3 Å, NTSR1-Gi-NTS(8-13) Complex in the Non-Canonical, AHD Open State (NC-Open-Apo)
8XXV 2.33 Å, Cryo-EM Structure of the Prostaglandin D2 Receptor 2-indomethacin Coupled to G Protein
3UMS 2.34 Å, Crystal structure of the G202A mutant of human G-alpha-i1
3ONW 2.38 Å, Structure of a G-alpha-i1 mutant with enhanced affinity for the RGS14 GoLoco motif.
20ZG 2.4 Å, Cryo-EM structure of the human neurotensin receptor 1 (hNTSR1)-Gi1 complex in the…

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