7UI9: Core Mediator-PICearly

Core Mediator-PICearly (Copy A). Determined by electron microscopy at 3.3 Å resolution. Released 15 Feb 2023.

Method
Electron microscopy
Resolution
3.3 Å
Organism
Saccharomyces cerevisiae S288C
Chains
33
Atoms
63,137
Mol. weight
1247.86 kDa
Released
15 Feb 2023

Explore 7UI9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7UI9 contains 349 α-helices and 337 β-strands across 33 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain a: 12 helices, 22 β-strands

ElementResiduesLengthSheet
α-helix7-2115
α-helix28-3710
β-strand41-46617
β-strand51-57717
β-strand61-68817
β-strand73-81917
β-strand91118
β-strand102118
α-helix103-1097
α-helix115-13016
α-helix164-18118
β-strand187-190419
β-strand198-203619
β-strand209-2191119
β-strand227-229320
β-strand232-234321
β-strand239-241321
β-strand248-250320
β-strand252-258719
β-strand272-274322
α-helix280-2823
β-strand283-286419
α-helix298-3047
β-strand312-316523
β-strand322-324322
β-strand325-329519
α-helix332-3365
α-helix337-34711
α-helix348-3525
α-helix353-3608
β-strand528-532523
β-strand536-539423
β-strand543-546423
α-helix551-56515
Chain A: 82 helices, 71 β-strands
ElementResiduesLengthSheet
α-helix101
β-strand11142
β-strand14-18543
α-helix24-307
β-strand34144
β-strand41145
β-strand48145
α-helix491
β-strand82-911044
α-helix96-1049
β-strand106146
β-strand113146
α-helix120-1267
α-helix131-14212
β-strand147147
β-strand151-153348
β-strand161-163348
β-strand170147
β-strand173-177549
β-strand180-185649
α-helix189-1913
β-strand198-201449
α-helix202-2032
α-helix204-2129
α-helix216-2227
α-helix232-2343
β-strand235-241744
α-helix248-2492
β-strand250-253450
β-strand256-258350
α-helix261-28020
α-helix286-30419
α-helix312-3143
β-strand315151
α-helix3161
α-helix3201
β-strand321151
α-helix3221
α-helix326-3294
α-helix3351
α-helix336-3405
β-strand343-345352
β-strand348-352553
β-strand355154
β-strand363-367553
α-helix368-3736
β-strand375-37952
α-helix3801
α-helix385-39410
β-strand402-40652
β-strand412-41432
α-helix424-4263
β-strand431-43552
α-helix4361
β-strand441-445553
α-helix452-4543
β-strand455-463953
β-strand469153
β-strand470154
β-strand486-490553
α-helix495-5039
β-strand506153
α-helix507-5093
β-strand512-513255
β-strand518-519255
α-helix525-53410
β-strand540-541256
α-helix543-55210
α-helix559-5635
β-strand565-567311
β-strand571111
β-strand572-573256
α-helix574-5785
β-strand588-590357
β-strand605-608457
β-strand611-614457
α-helix619-6224
α-helix629-6368
α-helix639-66022
α-helix666-6683
α-helix673-69725
α-helix702-7043
α-helix710-73627
α-helix742-7498
α-helix755-7584
α-helix759-7635
β-strand766-767258
β-strand770159
β-strand773159
α-helix794-7963
β-strand799-800258
α-helix810-84536
β-strand849-850260
β-strand856-857260
β-strand863-865360
α-helix868-8703
β-strand873161
β-strand878-882562
α-helix890-8978
α-helix904-9063
α-helix907-9093
β-strand913163
α-helix916-9194
α-helix924-94623
β-strand953-957562
α-helix960-97011
β-strand979163
α-helix983-99513
α-helix1005-102521
α-helix1028-10292
α-helix1030-10356
α-helix1039-105416
β-strand1057161
α-helix1058-10592
α-helix1064-107815
α-helix1083-10886
α-helix1090-10923
α-helix1098-11069
β-strand1116-1119412
β-strand1120164
α-helix1128-113811
β-strand1141-1142265
α-helix1143-11453
β-strand1147-1154866
α-helix1164-117411
α-helix1179-11846
α-helix1185-11873
β-strand1191-1197766
α-helix1199-12046
α-helix1209-12102
α-helix1211-12155
α-helix1216-12216
β-strand1224-1228566
β-strand1236-1242766
α-helix1250-12545
α-helix1256-126914
β-strand1272-1274365
β-strand1281-12921212
β-strand1298-13091212
α-helix1313-13164
β-strand1322164
β-strand1328-1329212
α-helix1332-13387
α-helix1341-135717
α-helix1365-137511
α-helix1386-13894
α-helix1396-14005
α-helix1405-141511
β-strand1418-1419243
α-helix1424-14296
β-strand1437167
β-strand1441-144553
α-helix1447-14526
Chain B: 52 helices, 72 β-strands
ElementResiduesLengthSheet
α-helix27-4014
α-helix45-539
α-helix54-585
α-helix59-657
β-strand69-73568
α-helix80-823
β-strand84-971468
α-helix98-1003
β-strand101-103369
β-strand109-111369
α-helix114-1207
β-strand125-1401668
β-strand150-156768
α-helix161-1633
β-strand164-171868
β-strand173170
α-helix180-1834
α-helix186-1916
β-strand203-205370
β-strand208-212570
β-strand214-218571
β-strand224-227472
β-strand234-241872
β-strand253-259772
β-strand268-272572
β-strand280-281272
α-helix282-2898
α-helix294-3018
α-helix309-32113
α-helix327-33610
α-helix345-35915
α-helix371-38919
α-helix395-3973
α-helix401-4033
β-strand404-407471
α-helix409-43729
α-helix441-4433
α-helix444-4474
α-helix451-46313
β-strand466173
β-strand476173
β-strand480-482370
α-helix483-4842
α-helix488-4947
β-strand497-499371
α-helix516-5183
β-strand522174
β-strand536-538371
β-strand539174
β-strand544-545275
α-helix552-5609
α-helix5631
β-strand564-565276
α-helix566-5683
α-helix5771
β-strand578-582576
β-strand585-590676
α-helix593-60513
β-strand614-618576
β-strand623-627576
β-strand633-638675
β-strand640-643477
β-strand648-651477
α-helix655-66713
α-helix681-6866
β-strand690-694575
α-helix697-7004
β-strand703-704278
α-helix707-7104
β-strand740-741278
α-helix745-7484
α-helix759-7613
α-helix764-77310
β-strand792-796528
β-strand800179
β-strand804-805280
α-helix809-8124
β-strand821-827781
β-strand838-842581
α-helix843-8475
β-strand853-8621028
β-strand873-874282
β-strand882-883282
α-helix890-8923
β-strand898183
β-strand904-905228
β-strand910184
β-strand912183
β-strand914-917482
β-strand934-936382
β-strand940184
α-helix941-9422
β-strand947-9561028
β-strand962-9721128
β-strand980-982381
β-strand987-994881
β-strand1001-1002285
β-strand1010-1012381
α-helix1014-10163
α-helix1023-103816
α-helix10411
β-strand1042-1043280
α-helix1052-10609
β-strand1069-1070281
β-strand1072-1073285
β-strand1080185
β-strand1085-10941081
α-helix1099-11013
β-strand1104-1106353
α-helix1118-11192
β-strand1128-1130352
α-helix1132-114110
β-strand1143167
α-helix1144-11485
α-helix1149-11535
β-strand1158-1163642
β-strand1172-1174386
β-strand1179-1181386
β-strand1182187
β-strand1187187
β-strand1191-1196642
α-helix1198-120811
β-strand1215-1218443
α-helix1219-12224
Chain C: 12 helices, 12 β-strands
ElementResiduesLengthSheet
β-strand7-13788
β-strand17-23788
α-helix27-3913
β-strand43-541289
α-helix60-678
β-strand72190
α-helix77-793
β-strand97-104889
β-strand111-114491
β-strand119-120289
α-helix1311
β-strand132190
α-helix1331
β-strand143-147591
β-strand152-1621189
α-helix168-1703
β-strand173-179788
α-helix197-2004
α-helix202-2043
α-helix205-2095
α-helix211-2144
α-helix218-2192
β-strand228-234788
α-helix240-27031
Chain d: 8 helices, 0 β-strands
ElementResiduesLengthSheet
α-helix32-398
α-helix41-6121
α-helix67-8822
α-helix92-12736
α-helix132-14716
α-helix150-16415
α-helix185-1906
α-helix192-1987
Chain D: 10 helices, 3 β-strands
ElementResiduesLengthSheet
β-strand36-38392
β-strand44-46392
β-strand4917
α-helix52-7726
α-helix111-1144
α-helix119-13315
α-helix139-15012
α-helix157-16812
α-helix174-1829
α-helix188-1947
α-helix196-1983
α-helix204-21714
α-helix219-2202
Chain E: 13 helices, 14 β-strands
ElementResiduesLengthSheet
α-helix3-2523
β-strand28193
α-helix32-354
α-helix39-457
β-strand47194
β-strand53194
α-helix55-584
β-strand60-62395
β-strand64193
α-helix66-716
β-strand78-82595
β-strand87-88296
α-helix90-10314
β-strand107-112695
β-strand115-116296
α-helix118-1214
α-helix124-1263
β-strand131-136695
α-helix137-1393
α-helix144-1463
β-strand152-155497
α-helix158-16811
α-helix172-1743
β-strand177-178297
α-helix183-1886
β-strand195-202897
β-strand206-214997
Chain f: 6 helices, 6 β-strands
ElementResiduesLengthSheet
α-helix14-196
α-helix27-326
α-helix42-5514
α-helix89-968
α-helix98-10710
β-strand113-121926
β-strand125-1341026
β-strand146-1561126
β-strand159-161326
α-helix166-19126
β-strand193-195327
β-strand199-201327

25 more chain groups are not listed. Open the entry in the viewer and use the sequence panel to see them.

Molecules and chains

MoleculeChainsTypeLengthOrganismUniProt
Transcription initiation factor IIBMprotein345Saccharomyces cerevisiae S288CP29055 (AlphaFold model)
Transcription initiation factor IIF subunit alphaPprotein735Saccharomyces cerevisiae S288CP41895 (AlphaFold model)
Transcription initiation factor IIF subunit betaQprotein400Saccharomyces cerevisiae S288CP41896 (AlphaFold model)
Transcription elongation factor S-IISprotein309Saccharomyces cerevisiae S288CP07273 (AlphaFold model)
Mediator of RNA polymerase II transcription subunit 1aprotein566Saccharomyces cerevisiae S288CQ12321
Mediator of RNA polymerase II transcription subunit 4dprotein284Saccharomyces cerevisiae S288CQ12343
Mediator of RNA polymerase II transcription subunit 6fprotein295Saccharomyces cerevisiae S288CP38782
Mediator of RNA polymerase II transcription subunit 7gprotein222Saccharomyces cerevisiae S288CQ08278
Mediator of RNA polymerase II transcription subunit 8hprotein223Saccharomyces cerevisiae S288CP38304
Mediator of RNA polymerase II transcription subunit 9iprotein149Saccharomyces cerevisiae S288CP33308
Mediator of RNA polymerase II transcription subunit 10jprotein157Saccharomyces cerevisiae S288CQ06213
Mediator of RNA polymerase II transcription subunit 11kprotein115Saccharomyces cerevisiae S288CQ99278

21 more molecules are not listed.

Sequence of entity 1 (M), FASTA
>7UI9_1 Transcription initiation factor IIB (chains M)
MMTRESIDKRAGRRGPNLNIVLTCPECKVYPPKIVERFSEGDVVCALCGLVLSDKLVDTR
SEWRTFSNDDHNGDDPSRVGEASNPLLDGNNLSTRIGKGETTDMRFTKELNKAQGKNVMD
KKDNEVQAAFAKITMLCDAAELPKIVKDCAKEAYKLCHDEKTLKGKSMESIMAASILIGC
RRAEVARTFKEIQSLIHVKTKEFGKTLNIMKNILRGKSEDGFLKIDTDNMSGAQNLTYIP
RFCSHLGLPMQVTTSAEYTAKKCKEIKEIAGKSPITIAVVSIYLNILLFQIPITAAKVGQ
TLQVTEGTIKSGYKILYEHRDKLVDPQLIANGVVSLDNLPGVEKK
Sequence of entity 2 (P), FASTA
>7UI9_2 Transcription initiation factor IIF subunit alpha (chains P)
MSRRNPPGSRNGGGPTNASPFIKRDRMRRNFLRMRMGQNGSNSSSPGVPNGDNSRGSLVK
KDDPEYAEEREKMLLQIGVEADAGRSNVKVKDEDPNEYNEFPLRAIPKEDLENMRTHLLK
FQSKKKINPVTDFHLPVRLHRKDTRNLQFQLTRAEIVQRQKEISEYKKKAEQERSTPNSG
GMNKSGTVSLNNTVKDGSQTPTVDSVTKDNTANGVNSSIPTVTGSSVPPASPTTVSAVES
NGLSNGSTSAANGLDGNASTANLANGRPLVTKLEDAGPAEDPTKVGMVKYDGKEVTNEPE
FEEGTMDPLADVAPDGGGRAKRGNLRRKTRQLKVLDENAKKLRFEEFYPWVMEDFDGYNT
WVGSYEAGNSDSYVLLSVEDDGSFTMIPADKVYKFTARNKYATLTIDEAEKRMDKKSGEV
PRWLMKHLDNIGTTTTRYDRTRRKLKAVADQQAMDEDDRDDNSEVELDYDEEFADDEEAP
IIDGNEQENKESEQRIKKEMLQANAMGLRDEEAPSENEEDELFGEKKIDEDGERIKKALQ
KTELAALYSSDENEINPYLSESDIENKENESPVKKEEDSDTLSKSKRSSPKKQQKKATNA
HVHKEPTLRVKSIKNCVIILKGDKKILKSFPEGEWNPQTTKAVDSSNNASNTVPSPIKQE
EGLNSTVAEREETPAPTITEKDIIEAIGDGKVNIKEFGKFIRRKYPGAENKKLMFAIVKK
LCRKVGNDHMELKKE
Sequence of entity 3 (Q), FASTA
>7UI9_3 Transcription initiation factor IIF subunit beta (chains Q)
MSSGSAGAPALSNNSTNSVAKEKSGNISGDEYLSQEEEVFDGNDIENNETKVYEESLDLD
LERSNRQVWLVRLPMFLAEKWRDRNNLHGQELGKIRINKDGSKITLLLNENDNDSIPHEY
DLELTKKVVENEYVFTEQNLKKYQQRKKELEADPEKQRQAYLKKQEREEELKKKQQQQKR
RNNRKKFNHRVMTDRDGRDRYIPYVKTIPKKTAIVGTVCHECQVMPSMNDPNYHKIVEQR
RNIVKLNNKERITTLDETVGVTMSHTGMSMRSDNSNFLKVGREKAKSNIKSIRMPKKEIL
DYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLDKVATLVKKGPYAFKYTLRPEYKKLKE
EERKATLGELADEQTGSAGDNAQGDAEADLEDEIEMEDVV
Sequence of entity 4 (S), FASTA
>7UI9_4 Transcription elongation factor S-II (chains S)
MDSKEVLVHVKNLEKNKSNDAAVLEILHVLDKEFVPTEKLLRETKVGVEVNKFKKSTNVE
ISKLVKKMISSWKDAINKNKRSRQAQQHHQDHAPGNAEDKTTVGESVNGVQQPASSQSDA
MKQDKYVSTKPRNSKNDGVDTAIYHHKLRDQVLKALYDVLAKESEHPPQSILHTAKAIES
EMNKVNNCDTNEAAYKARYRIIYSNVISKNNPDLKHKIANGDITPEFLATCDAKDLAPAP
LKQKIEEIAKQNLYNAQGATIERSVTDRFTCGKCKEKKVSYYQLQTRSADEPLTTFCTCE
ACGNRWKFS
Sequence of entity 5 (a), FASTA
>7UI9_5 Mediator of RNA polymerase II transcription subunit 1 (chains a)
MVEGDSYVETLDSMIELFKDYKPGSITLENITRLCQTLGLESFTEELSNELSRLSTASKI
IVIDVDYNKKQDRIQDVKLVLASNFDNFDYFNQRDGEHEKSNILLNSLTKYPDLKAFHNN
LKFLYLLDAYSHIESDSTSHNNGSSDKSLDSSNASFNNQGKLDLFKYFTELSHYIRQCFQ
DNCCDFKVRTNLNDKFGIYILTQGINGKEVPLAKIYLEENKSDSQYRFYEYIYSQETKSW
INESAENFSNGISLVMEIVANAKESNYTDLIWFPEDFISPELIIDKVTCSSNSSSSPPII
DLFSNNNYNSRIQLMNDFTTKLINIKKFDISNDNLDLISEILKWVQWSRIVLQNVFKLVS
TPSSNSNSSELEPDYQAPFSTSTKDKNSSTSNTEPIPRSNRHGSVVEASRRRRSSTNKSK
RPSITEAMMLKEEGLQQFNLHEILSEPAIEEENGDSIKEHSTTMDGANDLGFTASVSNQE
NAGTDIVMEDHGVLQGTSQNYGTATADDADIEMKDVSSKPSKPESSVLQLIVSEDHIILD
TISECNLYDDVKCWSKFIEKFQDIVS
Sequence of entity 6 (d), FASTA
>7UI9_6 Mediator of RNA polymerase II transcription subunit 4 (chains d)
MSVQDTKAVEFSMGHIRSSSVSLVAEATSNTNSEDKLSKVQLYEDLCRYEDTLSKLVESV
DRFKPNLDIAKDLIRTDEALFENVKLLAEYDNIYRNLQKIDKDSEELDSKTRKILEILNE
CHDELKALPMLEQVEFEKNTILQQRSKINSTELLDYATKLSKFTKIPPTFDKGAVGPNNF
IWPAEDALRRGMLAMASLHSKELTRIPGEEVEETEVPTVPPSQSEEQKGQMAKKEGTPKT
DSFIFDGTAKEVGDEADNTKDKEKEENNDDALDLDLDLFDPDDF
Sequence of entity 7 (f), FASTA
>7UI9_7 Mediator of RNA polymerase II transcription subunit 6 (chains f)
MNVTPLDELQWKSPEWIQVFGLRTENVLDYFAESPFFDKTSNNQVIKMQRQFSQLNDPNA
AVNMTQNIMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSV
REPDFWVIRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYH
LNSTLESLYDLIEFQPSQGVHYKVPTDTSTTATAATNGNNAGGGSNKSSVRPTGGANMAT
VPSTTNVNMTVNTMGTGGQTIDNGTGRTGNGNMGITTEMLDKLMVTSIRSTPNYI
Sequence of entity 8 (g), FASTA
>7UI9_8 Mediator of RNA polymerase II transcription subunit 7 (chains g)
MSNDPGNEVSSLYPPPPPYVKFFTQSNLEKLPKYKEKKAASAKQTAPNNSNGGSEEEITC
ALDYLIPPPMPKNQQYRAFGSIWQVKDQLPDLESMGLTQLYKKSTENESTNYQYKIQELR
KLLKSLLLNYLELIGVLSINPDMYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEE
QLEYKRGEIREIEQVCKQVHDKLTSIQDTLRTGSQSPPSSSQ
Sequence of entity 9 (h), FASTA
>7UI9_9 Mediator of RNA polymerase II transcription subunit 8 (chains h)
MSQSTASLVPEGNQGSLQEDVSFDFNGVPGQALDAVRMRLAQLTHSLRRIRDEMSKAELP
QWYTLQSQLNVTLSQLVSVTSTLQHFQETLDSTVVYPLPKFPTTSHESLVTTLLRKKNIP
EVDEWMKYVRETSGVTTALLKDEEIEKLLQQDREITNWARTTFRNEYGKHDFKNEESLSE
EHASLLVRDSKPSKPFNVDDVLKFTFTGEKPIITGSTSTSSSN
Sequence of entity 10 (i), FASTA
>7UI9_10 Mediator of RNA polymerase II transcription subunit 9 (chains i)
MNLQNNVLNQIHQILLPTNPTLDKPNAEATKEEFSSAENRDEKDYLTNQQPKNLSTPSTS
SNGEFIPHIFYSLHQIRKDPNNLSNQLETLTGSIRHRLKLCKSLISENEDTKDLLSKSPS
EWQDIIHQREQELQIKRDVLDDLYRKLQR
Sequence of entity 11 (j), FASTA
>7UI9_11 Mediator of RNA polymerase II transcription subunit 10 (chains j)
MNGNSTNNEQLQQELATTQDQVASIIESFVELGVSIYDFPGTPEATKGMITNLQRNVDRL
YKLNVRSNDPQSSLSKVDIPLEVVQYIEDGRNPDIYTREFVEAIRRSNQYQRGKMHGLKQ
LRDSLADKIVDEFPELKEPVEDIIKRTSPIDNVSNTH
Sequence of entity 12 (k), FASTA
>7UI9_12 Mediator of RNA polymerase II transcription subunit 11 (chains k)
MQPPYIQERLKSLNDIETQLCSMLQEASQVTFIFGELKRGNESVKPQFENHVKQFYERLD
KSTTQLRKEIQLLDENVGTRLLPINVNKKALGQDTEKMEEQLDLLSAILDPSKSK

Primary citation

Structural basis of a transcription pre-initiation complex on a divergent promoter. Gorbea Colon, J.J., Palao 3rd, L., Chen, S.F. et al. Mol Cell (2023) 83:574. DOI 10.1016/j.molcel.2023.01.011 · PubMed

Other PDB entries of the same protein (UniProt P29055 (AlphaFold model), which also has an AlphaFold model), best resolution first:

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