7WO9: Full-length Nup188

Cryo-EM structure of full-length Nup188. Determined by electron microscopy at 2.81 Å resolution. Released 30 Mar 2022.

Method

Electron microscopy

Resolution

2.81 Å

Organism

Saccharomyces cerevisiae S288C

Chains

1

Atoms

12,743

Mol. weight

188.75 kDa

Released

30 Mar 2022

Explore 7WO9 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7WO9 contains 96 α-helices and 15 β-strands across 1 chain. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 96 helices, 15 β-strands

Molecules and chains

>7WO9_1 Nucleoporin NUP188 (chains A)
MATPSFGNSSPQLTFTHVANFMNDAAADVSAVDAKQLAQIRQFLKANKTNLIESLNTIRQ
NVTSSGDHNKLRSTIANLLQINVDNDPFFAQSEDLSHAVEFFMSERSSRLHIVYSLLVNP
DIDLETYSFIDNDRFNVVGKLISIISSVIQNYDIITASSLAHDYNNDQDMFTIVSLVQLK
KFSDLKFILQILQILNLMILNTKVPVDIVNQWFLQYQNQFVEFCRNINSTDKSIDTSSLQ
LYKFQNFQDLSYLSETLISRISSLFTITTILILGLNTSIAQFDIQSPLYMDTETFDTVNS
ALENDVATNIVNEDPIFHPMIHYSWSFILYYRRALQSSESFDDSDITKFALFAESHDVLQ
KLNTLSEILSFDPVYTTVITVFLEFSLNFIPITASTSRVFAKIISKAPEQFIENFLTNDT
FEKKLSIIKAKLPLLNESLIPLINLALIDTEFANFELKDICSFAVTKSSLNDLDYDLIAD
TITNSSSSSDIIVPDLIELKSDLLVAPPLENENSNCLLSIPKSTKGKILTIKQQQQQQQQ
QNGQQPPTTSNLIIFLYKFNGWSLVGRILQNLLHSYMEKGTQLDDLQHELMISIIKLVTN
VVDPKTSIEKSSEILSYLSNSLDTSASTINGASIIQVIFEIFEISLQRKDYTSIVQCCEF
MTMLTPNYLHLVSSYLNKSDLLDKYGKTGLSNMILGSVELSTGDYTFTIQLLKLTKVFIR
ESLSLKNIHISKRSKIDIINKLILHAIHIFESYYNWKYNNFLQKFEIAFHLTLIFYDVLH
DVFTINPHQKDQLIISSSANKLLQLFLTPMDSIDLAPNTLTNILISPLNTTTKILGDKIL
GNLYSKVMNNSFKLCTLLIAIRGSNRDLKPSNLEKLLFINSSKLVDVYTLPSYVHFKVQI
IELLSYLVEAPWNDDYPFLLSFLGEAKSMAFLKEVLSDLSSPVQDWNLLRSLYIFFTTLL
ESKQDGLSILFLTGQFASNKKINDESSIDKKSSILTVLQKNSLLLDSTPEEVSCKLLETI
TYVLNTWTNSKIFIKDPKFVNSLLAKLKDSKKLFQKKENLTRDETVSLIKKYKLISRIVE
IFALCIYNSTDSNSEILNFLNQEDLFELVHHFFQIDGFNKTFHDELNLKFKEKWPSLELQ
SFQKIPLSRINENENFGYDIPLLDIVLKADRSWNEPSKSQTNFKEEITDASLNLQYVNYE
ISTAKAWGALITTFVKRSTVPLNDGFVDLVEHFLKLNIDFGSDKQMFTQIYLERIELSFY
ILYSFKLSGKLLKEEKIIELMNKIFTIFKSGEIDFIKNIGKSLKNNFYRPLLRSVLVLLE
LVSSGDRFIELISDQLLEFFELVFSKGVYLILSEILCQINKCSTRGLSTDHTTQIVNLED
NTQDLLLLLSLFKKITNVNPSKNFNVILASSLNEVGTLKVILNLYSSAHLIRINDEPILG
QITLTFISELCSIEPIAAKLINSGLYSVLLESPLSVAIQQGDIKPEFSPRLHNIWSNGLL
SIVLLLLSQFGIKVLPETCLFVSYFGKQIKSTIYNWGDNKLAVSSSLIKETNQLVLLQKM
LNLLNYQELFIQPKNSDDQQEAVELVIGLDSEHDKKRLSAALSKFLTHPKYLNSRIIPTT
LEEQQQLEDESSRLEFVKGISRDIKALQDSLFKDV

Primary citation

Near-atomic structure of the inner ring of the Saccharomyces cerevisiae nuclear pore complex. Li, Z., Chen, S., Zhao, L. et al. Cell Res (2022) 32:437-450. DOI 10.1038/s41422-022-00632-y · PubMed

Related structures

Other PDB entries of the same protein (UniProt P52593 (AlphaFold model), which also has an AlphaFold model), best resolution first:

• 7WOO 3.71 Å, Cryo-EM structure of the inner ring protomer of the Saccharomyces cerevisiae nuclear…
• 7WOT 3.73 Å, Cryo-EM structure of the inner ring monomer of the Saccharomyces cerevisiae nuclear pore…
• 8TJ5 6.6 Å, Inner spoke ring of the yeast NPC
• 7N85 7.6 Å, Inner ring spoke from the isolated yeast NPC
• 7N84 11.6 Å, Double nuclear outer ring from the isolated yeast NPC
• 7N9F 37.0 Å, Structure of the in situ yeast NPC
• 8ZZA Integrative structure and functional anatomy of a single spoke of a nuclear pore complex
• 8ZZB Integrative structure and functional anatomy of three spokes of a nuclear pore complex
• 8ZZC Integrative structure and functional anatomy of eight spokes of a nuclear pore complex
• 9A0F Integrative model of the wild type yeast nuclear pore complex
• 9A0G Integrative model of Nup116 knockout (at 25C) yeast nuclear pore complex
• 9A0H Integrative model of Nup116 knockout (at 37C) yeast nuclear pore complex

Browse structure collections

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