7WY8: ADGRL3/Gs complex

ADGRL3/Gs complex. Determined by electron microscopy at 2.83 Å resolution. Released 26 Oct 2022.

Method: Electron microscopy
Resolution: 2.83 Å
Organisms: Mus musculus, Homo sapiens, Lama glama
Chains: 5
Atoms: 8,049
Mol. weight: 277.08 kDa
Released: 26 Oct 2022

Explore 7WY8 in 3D Show helices and sheets RCSB PDB PDBe

Secondary structure: helices and β-sheets

7WY8 contains 38 α-helices and 48 β-strands across 5 chains. Residue ranges use author residue numbering, as in the PDB file, from PDBe. To see them in 3D, choose the Cartoon representation with Secondary structure coloring: helices, sheets and coils get different colors.

Chain A: 11 helices, 6 β-strands

Element	Residues	Length	Sheet
α-helix	14-38	25
β-strand	40-46	7	2
α-helix	53-60	8
α-helix	61-63	3
β-strand	207-214	8	2
β-strand	217-224	8	2
α-helix	234-238	5
β-strand	243-249	7	2
α-helix	253-265	3
α-helix	266-277	12
β-strand	286-292	7	2
α-helix	294-303	10
α-helix	308-310	3
α-helix	313-317	5
α-helix	332-351	20
β-strand	359-363	5	2
α-helix	371-390	20

Chain B: 4 helices, 28 β-strands

Element	Residues	Length	Sheet
α-helix	4-23	20
α-helix	30-33	4
α-helix	34-36	3
α-helix	38-39	2
β-strand	47-51	5	5
β-strand	58-63	6	6
β-strand	69-74	6	6
β-strand	78-83	6	6
β-strand	89-94	6	6
β-strand	100-105	6	7
β-strand	111-116	6	7
β-strand	120-125	6	7
β-strand	134-140	7	7
β-strand	146-153	8	8
β-strand	156-161	6	8
β-strand	166-170	5	8
β-strand	175-180	6	8
β-strand	187-192	6	9
β-strand	198-203	6	9
β-strand	207-212	6	9
β-strand	218-222	5	9
β-strand	229-234	6	10
β-strand	240-245	6	10
β-strand	249-254	6	10
β-strand	259-264	6	10
β-strand	273-278	6	11
β-strand	284-289	6	11
β-strand	293-298	6	11
β-strand	303-308	6	11
β-strand	315-320	6	5
β-strand	327-331	5	5
β-strand	336-339	4	5

Chain C: 4 helices, 0 β-strands

Element	Residues	Length
α-helix	9-22	14
α-helix	30-43	14
α-helix	53-55	3
α-helix	56-58	3

Chain D: 2 helices, 12 β-strands

Element	Residues	Length	Sheet
β-strand	3-7	5	3
β-strand	10-12	3	4
β-strand	18-25	8	3
β-strand	34-39	6	4
β-strand	45	1	4
β-strand	49-51	3	4
β-strand	58-60	3	4
α-helix	62-64	3
β-strand	68-73	6	3
β-strand	78-83	6	3
α-helix	88-90	3
β-strand	92-97	6	4
β-strand	118	1	4
β-strand	122-126	5	4

Chain R: 17 helices, 2 β-strands

Element	Residues	Length	Sheet
α-helix	926-928	3
α-helix	941-955	15
α-helix	959-968	10
α-helix	971-973	3
α-helix	976-998	23
α-helix	1009-1037	29
α-helix	1042-1043	2
α-helix	1048-1055	8
α-helix	1057-1069	13
β-strand	1074	1	1
β-strand	1082	1	1
α-helix	1087-1089	3
α-helix	1090-1112	23
α-helix	1144-1151	8
α-helix	1156-1163	8
α-helix	1170-1180	11
α-helix	1183-1188	6
α-helix	1189-1193	5
α-helix	1196-1206	11

Molecules and chains

Molecule	Chains	Type	Length	Organism	UniProt
Isoform 3 of Adhesion G protein-coupled receptor L3	R	protein	1543	Mus musculus	Q80TS3 (AlphaFold model)
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	C	protein	71	Homo sapiens	P59768 (AlphaFold model)
engineered mini G alpha s subunit	A	protein	361	Homo sapiens
NB35	D	protein	161	Lama glama
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	B	protein	345	Homo sapiens	P62873 (AlphaFold model)

Sequence of entity 1 (R), FASTA

>7WY8_1 Isoform 3 of Adhesion G protein-coupled receptor L3 (chains R)
MWPPQLLILTMLLAPVVHGGKHNERHPALAAPLRHAERSPGGALPPRHLLQQPAAERSTA
HRGQGPRGAARGVRGPGAPGAQIAAQAFSRAPIPMAVVRRELSCESYPIELRCPGTDVIM
IESANYGRTDDKICDSDPAQMENIRCYLPDAYKIMSQRCNNRTQCAVVAGPDVFPDPCPG
TYKYLEVQYECVPYKVEQKVFLCPGLLKGVYQSEHLFESDHQSGAWCKDPLQASDKIYYM
PWTPYRTDTLTEYSSKDDFIAGRPTTTYKLPHRVDGTGFVVYDGALFFNKERTRNIVKFD
LRTRIKSGEAIIANANYHDTSPYRWGGKSDIDLAVDENGLWVIYATEQNNGKIVISQLNP
YTLRIEGTWDTAYDKRSASNAFMICGILYVVKSVYEDDDNEATGNKIDYIYNTDQSKDSL
VDVPFPNSYQYIAAVDYNPRDNLLYVWNNYHVVKYSLDFGPLDSRSGPVHHGQVSYISPP
IHLDSELERPPVRGISTTGSLGMGSTTTSTTLRTTTWNIGRSTTASLPGRRNRSTSTPSP
AVEVLDDVTTHLPSAASQIPAMEESCEAVEAREIMWFKTRQGQVAKQPCPAGTIGVSTYL
CLAPDGIWDPQGPDLSNCSSPWVNHITQKLKSGETAANIARELAEQTRNHLNAGDITYSV
RAMDQLVGLLDVQLRNLTPGGKDSAARSLNKLQKRERSCRAYVQAMVETVNNLLQPQALN
AWRDLTTSDQLRAATMLLDTVEESAFVLADNLLKTDIVRENTDNIQLEVARLSTEGNLED
LKFPENMGHGSTIQLSANTLKQNGRNGEIRVAFVLYNNLGPYLSTENASMKLGTEAMSTN
HSVIVNSPVITAAINKEFSNKVYLADPVVFTVKHIKQSEENFNPNCSFWSYSKRTMTGYW
STQGCRLLTTNKTHTTCSCNHLTNFAVLMAHVEVKHSDAVHDLLLDVITWVGILLSLVCL
LICIFTFCFFRGLQSDRNTIHKNLCISLFVAELLFLIGINRTDQPIACAVFAALLHFFFL
AAFTWMFLEGVQLYIMLVEVFESEHSRRKYFYLVGYGMPALIVAVSAAVDYRSYGTDKVC
WLRLDTYFIWSFIGPATLIIMLNVIFLGIALYKMFHHTAILKPESGCLDNINYEDNRPFI
KSWVIGAIALLCLLGLTWAFGLMYINESTVIMAYLFTIFNSLQGMFIFIFHCVLQKKVRK
EYGKCLRTHCCSGKSTESSIGSGKTSGSRTPGRYSTGSQSRIRRMWNDTVRKQSESSFIT
GDINSSASLNREPYRETKGLLNNARDTSVMDTLPLNGNHGNSYSIAGGEYLSNCVQIIDR
GYNHNETALEKKILKELTSNYIPSYLNNHERSSEQNRNMMNKLVNNLGSGSEDDAIVLDD
AASFNHEESLGLELIHEESDAPLLPPRVYSTDNHQPHHYSRRRFPQDHSESFFPLLTDEH
TEDLQSPHRDSLYTSMPALAGVPAADSVTTSTQTEAAAAKGGDAEDVYYKSMPNLGSRNH
VHPLHAYYQLGRGSSDGFIVPPNKDGASPEGTSKGPAHLVTSL

Sequence of entity 2 (C), FASTA

>7WY8_2 Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 (chains C)
MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENP
FREKKFFCAIL

Sequence of entity 3 (A), FASTA

>7WY8_3 engineered mini G alpha s subunit (chains A)
MGCTLSAEDKAAVERSKMIEKQLQKDKQVYRATHRLLLLGADNSGKSTIVKQMRIYHVNG
YSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMT
AELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVK
TSGIFETKFQVDKVNFHMFDVGAQRDERRKWIQCFNDVTAIIFVVDSSDYNRLQEALNDF
KSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPR
VTRAKYFIRDEFLRISTASGDGRHYCYPHFTCSVDTENARRIFNDCRDIIQRMHLRQYEL
L

Sequence of entity 4 (D), FASTA

>7WY8_4 NB35 (chains D)
MKYLLPTAAAGLLLLAAQPAMAQVQLQESGGGLVQPGGSLRLSCAASGFTFSNYKMNWVR
QAPGKGLEWVSDISQSGASISYTGSVKGRFTISRDNAKNTLYLQMNSLKPEDTAVYYCAR
CPAPFTRDCFDVTSTTYAYRGQGTQVTVSSAAALEHHHHHH

Sequence of entity 5 (B), FASTA

>7WY8_5 Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 (chains B)
MGSLLQSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHL
AKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACG
GLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQ
QTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFF
PNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCN
VWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN

Primary citation

Structural insights into adhesion GPCR ADGRL3 activation and Gq, Gs, Gi, and G12 coupling. Qian, Y., Ma, Z., Liu, C. et al. Mol Cell (2022) 82:4340-4352.e6. DOI 10.1016/j.molcel.2022.10.009 · PubMed

Other PDB entries of the same protein (UniProt Q80TS3 (AlphaFold model), which also has an AlphaFold model), best resolution first:

4RML 1.6 Å, Crystal structure of the Olfactomedin domain of latrophilin 3 in C2221 crystal form
4RMK 1.61 Å, Crystal structure of the Olfactomedin domain of latrophilin 3 in P65 crystal form
6JBU 1.85 Å, High resolution crystal structure of human FLRT3 LRR domain in complex with mouse CIRL3…
7WY5 2.83 Å, ADGRL3/Gq complex
7X10 2.93 Å, ADGRL3/miniG12 complex
7WYB 2.97 Å, ADGRL3/Gi complex
4YEB 3.19 Å, Structural characterization of a synaptic adhesion complex
5FTT 3.4 Å, Octameric complex of Latrophilin 3 (Lec, Olf) , Unc5D (Ig, Ig2, TSP1) and FLRT2 (LRR)
5FTU 6.01 Å, Tetrameric complex of Latrophilin 3, Unc5D and FLRT2

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